Potassium in PDB 8cts: Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)

The structure of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K), PDB code: 8cts was solved by B.Lee, K.I.White, M.A.Socolich, M.A.Klureza, R.Henning, V.Srajer, R.Ranganathan, D.Hekstra, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.41 / 1.60
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	68.816, 68.816, 90.365, 90, 90, 90
R / Rfree (%)	14.1 / 15.4

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>>> Page 1 <<< Page 2, Binding sites: 11 - 11;

Binding sites:

The binding sites of Potassium atom in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) (pdb code 8cts). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 11 binding sites of Potassium where determined in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K), PDB code: 8cts:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Potassium binding site 1 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ A:K210 b:6.8 occ:0.25 OG1 A:THR63 2.7 6.0 1.0 HB A:THR63 3.1 6.4 1.0 O A:THR63 3.1 5.0 1.0 CB A:THR63 3.5 5.3 1.0 K A:K211 3.5 3.6 0.2 C A:THR63 3.9 3.8 1.0 CA A:THR63 4.3 4.3 1.0 HG23 A:THR63 4.6 9.9 1.0 CG2 A:THR63 4.7 8.2 1.0 HA A:VAL64 4.7 4.4 1.0 HA A:THR63 4.8 5.1 1.0 K A:K215 4.9 72.8 0.2 N A:VAL64 4.9 3.7 1.0

Potassium binding site 2 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ A:K211 b:3.6 occ:0.25 O A:THR63 2.6 5.0 1.0 O A:VAL64 2.9 3.8 1.0 K A:K212 3.2 3.3 0.2 K A:K210 3.5 6.8 0.2 HA A:VAL64 3.6 4.4 1.0 C A:VAL64 3.6 3.3 1.0 C A:THR63 3.8 3.8 1.0 CA A:VAL64 4.1 3.6 1.0 HA3 A:GLY65 4.3 3.7 1.0 N A:VAL64 4.4 3.7 1.0 HB A:THR63 4.5 6.4 1.0 N A:GLY65 4.6 2.9 1.0 CA A:GLY65 4.9 3.0 1.0 CA A:THR63 5.0 4.3 1.0

Potassium binding site 3 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ A:K212 b:3.3 occ:0.25 O A:GLY65 2.8 2.7 1.0 O A:VAL64 2.8 3.8 1.0 K A:K211 3.2 3.6 0.2 HA3 A:GLY65 3.4 3.7 1.0 K A:K213 3.4 5.4 0.2 C A:GLY65 3.5 2.6 1.0 CA A:GLY65 3.9 3.0 1.0 C A:VAL64 4.0 3.3 1.0 HA A:TYR66 4.2 3.7 1.0 N A:GLY65 4.4 2.9 1.0 N A:TYR66 4.4 3.1 1.0 CA A:TYR66 4.8 3.1 1.0 HA2 A:GLY65 4.8 3.7 1.0

Potassium binding site 4 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ A:K213 b:5.4 occ:0.25 K A:K214 2.6 74.3 0.2 O A:TYR66 2.7 3.5 1.0 O A:GLY65 3.1 2.7 1.0 C A:TYR66 3.4 3.3 1.0 K A:K212 3.4 3.3 0.2 HA A:TYR66 3.6 3.7 1.0 HA3 A:GLY67 3.9 4.6 1.0 CA A:TYR66 4.0 3.1 1.0 C A:GLY65 4.2 2.6 1.0 N A:GLY67 4.3 3.7 1.0 CA A:GLY67 4.6 3.8 1.0 N A:TYR66 4.6 3.1 1.0 O A:HOH312 4.7 40.1 1.0 H A:GLY67 4.9 4.5 1.0

Potassium binding site 5 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ A:K214 b:74.3 occ:0.25 O A:HOH312 2.5 40.1 1.0 K A:K213 2.6 5.4 0.2 O A:TYR66 2.9 3.5 1.0 HA3 A:GLY67 3.5 4.6 1.0 C A:TYR66 4.1 3.3 1.0 CA A:GLY67 4.4 3.8 1.0 O A:HOH323 4.5 51.9 0.2 N A:GLY67 4.7 3.7 1.0 C A:GLY67 4.8 6.1 1.0 O A:GLY67 5.0 7.4 1.0

Potassium binding site 6 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ A:K215 b:72.8 occ:0.25 HE2 A:PHE92 3.6 22.4 0.4 HZ A:PHE92 4.0 21.3 0.4 CE2 A:PHE92 4.2 18.7 0.4 CZ A:PHE92 4.4 17.7 0.4 OG1 A:THR63 4.8 6.0 1.0 K A:K210 4.9 6.8 0.2

Potassium binding site 7 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 7 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ B:K211 b:12.8 occ:0.25 O B:TYR66 2.7 12.3 1.0 O B:GLY65 3.1 8.9 1.0 C B:TYR66 3.4 12.6 1.0 K B:K215 3.5 8.0 0.2 HA B:TYR66 3.6 12.6 1.0 HA3 B:GLY67 3.9 14.7 1.0 CA B:TYR66 4.0 10.5 1.0 C B:GLY65 4.2 8.3 1.0 N B:GLY67 4.2 11.2 1.0 CA B:GLY67 4.6 12.3 1.0 N B:TYR66 4.6 9.7 1.0 H B:GLY67 4.9 13.4 1.0

Potassium binding site 8 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 8 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ B:K212 b:75.8 occ:0.25 HE2 B:PHE92 3.4 16.3 0.3 HZ B:PHE92 4.1 22.0 0.3 CE2 B:PHE92 4.1 13.6 0.3 CZ B:PHE92 4.5 18.3 0.3

Potassium binding site 9 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 9 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ B:K213 b:7.2 occ:0.25 OG1 B:THR63 2.7 6.0 1.0 HB B:THR63 3.0 7.5 1.0 O B:THR63 3.0 6.9 1.0 CB B:THR63 3.4 6.2 1.0 K B:K214 3.5 6.4 0.2 C B:THR63 3.9 6.0 1.0 CA B:THR63 4.3 5.4 1.0 HG21 B:THR63 4.6 10.7 1.0 CG2 B:THR63 4.7 8.9 1.0 HA B:VAL64 4.7 6.7 1.0 HA B:THR63 4.8 6.5 1.0 N B:VAL64 4.9 5.2 1.0

Potassium binding site 10 out of 11 in the Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 10 of Room Temperature Crystal Structure of A K+ Selective Nak Mutant (NAK2K) within 5.0Å range: probe atom residue distance (Å) B Occ B:K214 b:6.4 occ:0.25 O B:THR63 2.6 6.9 1.0 O B:VAL64 2.9 7.0 1.0 K B:K215 3.2 8.0 0.2 K B:K213 3.5 7.2 0.2 HA B:VAL64 3.6 6.7 1.0 C B:VAL64 3.6 6.4 1.0 C B:THR63 3.8 6.0 1.0 CA B:VAL64 4.0 5.6 1.0 HA3 B:GLY65 4.2 9.1 1.0 N B:VAL64 4.4 5.2 1.0 HB B:THR63 4.5 7.5 1.0 N B:GLY65 4.6 6.8 1.0 CA B:GLY65 4.8 7.6 1.0 CA B:THR63 4.9 5.4 1.0

B.Lee, K.I.White, M.A.Socolich, M.A.Klureza, R.Henning, V.Srajer, R.Ranganathan, D.Hekstra. Direct Visualization of Electric Field-Stimulated Ion Conduction in A Potassium Channel To Be Published.