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Potassium in PDB 6dpg: Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C, PDB code: 6dpg was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.95 / 1.38
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.645, 37.644, 61.962, 90.00, 96.64, 90.00
R / Rfree (%) 15.9 / 17.5

Other elements in 6dpg:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C also contains other interesting chemical elements:

Manganese (Mn) 4 atoms
Iodine (I) 3 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C (pdb code 6dpg). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C, PDB code: 6dpg:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 6dpg

Go back to Potassium Binding Sites List in 6dpg
Potassium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K101

b:30.8
occ:0.40
OP2 b:C5 2.5 20.8 0.4
OP2 b:C5 2.6 23.7 0.4
OP2 b:C5 2.6 23.6 0.2
O b:HOH208 2.8 38.1 1.0
C5' B:U4 2.8 19.4 0.4
OP1 B:U4 3.2 26.7 0.4
O5' B:U4 3.3 26.8 0.4
OP1 B:U4 3.4 25.9 0.2
O5' B:U4 3.4 25.4 0.2
HG1 A:THR183 3.4 28.8 1.0
HG21 A:THR183 3.5 31.2 1.0
OP1 B:U4 3.5 23.4 0.4
O A:HOH340 3.6 24.7 1.0
OD2 A:ASP132 3.6 30.2 1.0
C5' B:U4 3.6 19.4 0.2
O A:HOH321 3.6 23.4 1.0
O b:HOH201 3.6 33.7 1.0
P b:C5 3.6 21.7 0.2
P b:C5 3.7 23.2 0.4
P B:U4 3.7 27.0 0.2
P b:C5 3.7 17.7 0.4
P B:U4 3.8 21.6 0.4
O5' B:U4 3.9 24.4 0.4
C5' B:U4 3.9 27.7 0.4
OP2 B:U4 3.9 23.1 0.2
OP3 b:C5 4.0 20.8 0.4
HB2 A:ASP132 4.0 23.9 1.0
CG A:ASP132 4.1 22.8 1.0
O3' B:U4 4.1 23.0 0.2
OP1 b:C5 4.1 21.9 0.4
OP1 b:C5 4.1 20.1 0.2
C4' B:U4 4.1 21.9 0.4
P B:U4 4.1 23.5 0.4
C3' B:U4 4.1 21.7 0.2
O4' B:U4 4.2 22.9 0.4
OG1 A:THR183 4.2 24.0 1.0
OP3 b:C5 4.3 21.2 0.4
OP1 b:C5 4.3 16.2 0.4
CG2 A:THR183 4.3 26.0 1.0
OP2 B:U4 4.4 27.5 0.4
C4' B:U4 4.4 22.6 0.2
O b:HOH203 4.4 28.4 0.6
C3' B:U4 4.5 19.1 0.4
HG23 A:THR183 4.6 31.2 1.0
OD1 A:ASP132 4.6 21.6 1.0
CB A:ASP132 4.6 19.9 1.0
C6 B:U4 4.6 20.3 0.4
O b:HOH206 4.7 30.4 0.4
O A:HOH425 4.7 78.3 1.0
C4' B:U4 4.7 25.9 0.4
CB A:THR183 4.8 26.1 1.0
HB A:THR183 4.8 31.3 1.0
HB3 A:ASP132 4.9 23.9 1.0
C2' B:U4 4.9 19.3 0.4
O4' B:U4 4.9 23.2 0.4
MN A:MN212 4.9 20.3 1.0
MN A:MN211 5.0 19.8 0.9
O5' b:C5 5.0 22.5 0.4

Potassium binding site 2 out of 2 in 6dpg

Go back to Potassium Binding Sites List in 6dpg
Potassium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 240 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K101

b:51.7
occ:0.50
O C:HOH209 2.6 30.5 0.5
O6 C:DG5 2.6 28.6 1.0
O C:HOH207 3.0 38.8 1.0
O4 C:DT4 3.5 25.7 1.0
C6 C:DG5 3.7 25.1 1.0
O C:HOH218 3.9 52.2 1.0
O C:HOH205 4.1 46.8 1.0
N4 B:C2 4.1 37.2 1.0
O B:HOH305 4.1 59.0 1.0
N7 C:DG5 4.2 26.8 1.0
N6 B:A1 4.2 33.7 1.0
C5 C:DG5 4.3 26.9 1.0
C4 C:DT4 4.3 21.9 1.0
C7 C:DT4 4.4 27.1 1.0
N6 B:A3 4.7 25.6 1.0
C5 C:DT4 4.8 25.7 1.0
O4 C:DT6 4.9 30.3 1.0
N1 C:DG5 4.9 29.1 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Aug 12 15:53:35 2024

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