Potassium in PDB 3wrm: Crystal Structure of P450CAM

All present enzymatic activity of Crystal Structure of P450CAM:
1.14.15.1;

The structure of Crystal Structure of P450CAM, PDB code: 3wrm was solved by A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, T.Mizushima, H.Shimada, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.18 / 1.95
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	67.086, 62.543, 95.490, 90.00, 90.88, 90.00
R / Rfree (%)	17.7 / 23.3

The structure of Crystal Structure of P450CAM also contains other interesting chemical elements:

Iron

(Fe)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of P450CAM (pdb code 3wrm). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of P450CAM, PDB code: 3wrm:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:K502 b:10.5 occ:1.00 O A:GLU84 2.6 9.1 1.0 O A:TYR96 2.7 10.9 1.0 O A:GLU94 2.7 7.7 1.0 O A:GLY93 2.8 11.7 1.0 O A:HOH691 2.8 17.1 1.0 C A:GLU94 3.4 9.7 1.0 CA A:GLU94 3.7 10.8 1.0 C A:TYR96 3.8 9.4 1.0 C A:GLU84 3.8 10.1 1.0 C A:GLY93 3.8 10.0 1.0 N A:TYR96 4.1 9.1 1.0 N A:GLU94 4.2 10.7 1.0 CA A:TYR96 4.4 9.5 1.0 N A:ALA95 4.4 10.5 1.0 CA A:CYS85 4.4 10.7 1.0 C A:ALA95 4.5 10.9 1.0 N A:CYS85 4.5 8.2 1.0 CG A:GLU84 4.6 22.5 1.0 CB A:TYR96 4.6 9.1 1.0 OE2 A:GLU84 4.6 42.7 1.0 N A:ASP97 4.8 9.7 1.0 CD A:GLU84 4.8 37.0 1.0 CB A:CYS85 4.9 12.3 1.0 CA A:GLU84 4.9 9.8 1.0 CA A:ALA95 4.9 10.9 1.0 O A:ALA95 5.0 10.5 1.0 CB A:GLU94 5.0 11.3 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ F:K502 b:16.6 occ:1.00 O F:GLU84 2.5 17.9 1.0 O F:TYR96 2.7 15.3 1.0 O F:HOH723 2.9 29.4 1.0 O F:GLY93 2.9 15.0 1.0 O F:GLU94 3.0 14.8 1.0 C F:GLU94 3.6 16.4 1.0 C F:GLU84 3.7 18.3 1.0 C F:TYR96 3.8 15.0 1.0 CA F:GLU94 3.8 17.1 1.0 C F:GLY93 4.0 15.5 1.0 N F:TYR96 4.2 17.5 1.0 CA F:CYS85 4.3 18.5 1.0 N F:GLU94 4.4 17.1 1.0 CG F:GLU84 4.4 32.7 1.0 CA F:TYR96 4.5 16.0 1.0 N F:CYS85 4.5 18.0 1.0 C F:ALA95 4.6 17.2 1.0 N F:ALA95 4.6 15.2 1.0 CB F:TYR96 4.7 15.7 1.0 OE1 F:GLU84 4.8 48.4 1.0 CA F:GLU84 4.8 19.4 1.0 CB F:CYS85 4.8 18.2 1.0 N F:ASP97 4.8 14.2 1.0

A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, Y.Katayama, R.Aminaka, M.Ito, T.Mizushima, H.Shimada. Structure of P450CAM Intermedite To Be Published.