Potassium in PDB 3wrj: Crystal Structure of P450CAM

All present enzymatic activity of Crystal Structure of P450CAM:
1.14.15.1;

The structure of Crystal Structure of P450CAM, PDB code: 3wrj was solved by A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, T.Mizushima, H.Shimada, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.92 / 1.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	66.885, 62.835, 95.731, 90.00, 90.83, 90.00
R / Rfree (%)	17.3 / 22.1

The structure of Crystal Structure of P450CAM also contains other interesting chemical elements:

Iron

(Fe)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of P450CAM (pdb code 3wrj). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of P450CAM, PDB code: 3wrj:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:K502 b:9.0 occ:1.00 O A:GLU84 2.6 9.8 1.0 O A:TYR96 2.7 10.8 1.0 O A:GLY93 2.7 11.4 1.0 O A:GLU94 2.8 9.5 1.0 O A:HOH754 2.8 18.6 1.0 C A:GLU94 3.4 10.7 1.0 CA A:GLU94 3.7 10.1 1.0 C A:TYR96 3.8 9.0 1.0 C A:GLU84 3.8 10.9 1.0 C A:GLY93 3.8 9.2 1.0 N A:TYR96 4.1 9.3 1.0 N A:GLU94 4.2 10.3 1.0 N A:ALA95 4.4 8.6 1.0 CA A:CYS85 4.4 11.6 1.0 CA A:TYR96 4.4 9.4 1.0 C A:ALA95 4.4 10.6 1.0 N A:CYS85 4.5 9.7 1.0 CB A:TYR96 4.6 8.9 1.0 CG A:GLU84 4.6 21.0 1.0 N A:ASP97 4.8 8.4 1.0 CA A:GLU84 4.9 9.9 1.0 CA A:ALA95 4.9 9.6 1.0 CB A:CYS85 4.9 11.9 1.0 O A:ALA95 5.0 10.9 1.0 CB A:GLU94 5.0 10.9 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ E:K502 b:13.4 occ:1.00 O E:GLU84 2.5 16.0 1.0 O E:TYR96 2.7 13.2 1.0 O E:GLY93 2.8 13.9 1.0 O E:GLU94 2.9 14.5 1.0 C E:GLU94 3.5 15.3 1.0 C E:GLU84 3.7 15.1 1.0 CA E:GLU94 3.7 15.6 1.0 C E:TYR96 3.8 13.2 1.0 C E:GLY93 3.9 14.1 1.0 OE2 E:GLU84 4.0 31.1 0.5 N E:TYR96 4.2 14.8 1.0 N E:GLU94 4.3 14.7 1.0 CA E:CYS85 4.4 15.2 1.0 CG E:GLU84 4.4 20.6 0.5 CA E:TYR96 4.5 14.5 1.0 N E:ALA95 4.5 14.6 1.0 N E:CYS85 4.5 15.8 1.0 C E:ALA95 4.5 16.6 1.0 CD E:GLU84 4.6 24.3 0.5 CB E:TYR96 4.7 12.1 1.0 CA E:GLU84 4.8 15.3 0.5 CA E:GLU84 4.8 15.5 0.5 N E:ASP97 4.8 12.4 1.0 CB E:CYS85 4.9 16.5 1.0 O E:ALA95 4.9 16.0 1.0

A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, Y.Katayama, R.Aminaka, M.Ito, T.Mizushima, H.Shimada. Structure of P450CAM Intermedite To Be Published.