Potassium in PDB 6by2: Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant

The structure of Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant, PDB code: 6by2 was solved by A.J.Labro, D.M.Cortes, C.Tilegenova, L.G.Cuello, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.83 / 2.35
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	157.178, 157.178, 75.459, 90.00, 90.00, 90.00
R / Rfree (%)	22.1 / 24.5

The binding sites of Potassium atom in the Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant (pdb code 6by2). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant, PDB code: 6by2:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in the Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:K1002 b:57.6 occ:0.25 O C:GLY77 2.7 30.7 1.0 O C:HOH1124 3.0 58.2 0.2 C C:GLY77 3.4 36.5 1.0 CA C:GLY77 3.7 29.6 1.0 N C:GLY79 4.4 35.6 1.0 N C:TYR78 4.5 34.7 1.0 C C:TYR78 4.5 36.4 1.0 CA C:GLY79 4.7 34.1 1.0 O C:TYR78 4.8 32.9 1.0 CA C:TYR78 4.9 37.9 1.0

Potassium binding site 2 out of 3 in the Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:K1003 b:33.5 occ:0.25 O C:ALA75 2.7 31.7 1.0 O C:HOH1126 3.4 63.8 0.2 C C:ALA75 3.9 36.3 1.0 CB C:ALA75 4.0 32.3 1.0 CA C:ALA75 4.5 38.3 1.0 C C:VAL76 4.9 33.5 1.0 N C:VAL76 5.0 32.7 1.0

Potassium binding site 3 out of 3 in the Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Closed and Deep-Inactivated Conformation of Kcsa-T75A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:K1004 b:87.6 occ:0.25 O C:HOH1126 3.5 63.8 0.2 O C:HOH1125 3.6 60.5 1.0 O C:HOH1121 3.9 55.4 1.0

A.J.Labro, D.M.Cortes, C.Tilegenova, L.G.Cuello. Inverted Allosteric Coupling Between Activation and Inactivation Gates in K+Channels. Proc. Natl. Acad. Sci. V. 115 5426 2018U.S.A..
ISSN: ESSN 1091-6490
PubMed: 29735651
DOI: 10.1073/PNAS.1800559115