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Potassium in PDB 1f3x: S402P Mutant of Rabbit Muscle Pyruvate Kinase

Enzymatic activity of S402P Mutant of Rabbit Muscle Pyruvate Kinase

All present enzymatic activity of S402P Mutant of Rabbit Muscle Pyruvate Kinase:
2.7.1.40;

Protein crystallography data

The structure of S402P Mutant of Rabbit Muscle Pyruvate Kinase, PDB code: 1f3x was solved by J.O.Wooll, R.H.E.Friesen, M.A.White, S.J.Watowich, R.O.Fox, J.C.Lee, E.W.Czerwinski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 36.00 / 2.80
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 82.138, 108.707, 144.387, 95.26, 93.47, 112.24
R / Rfree (%) 24.1 / 27

Other elements in 1f3x:

The structure of S402P Mutant of Rabbit Muscle Pyruvate Kinase also contains other interesting chemical elements:

Manganese (Mn) 8 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the S402P Mutant of Rabbit Muscle Pyruvate Kinase (pdb code 1f3x). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 8 binding sites of Potassium where determined in the S402P Mutant of Rabbit Muscle Pyruvate Kinase, PDB code: 1f3x:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Potassium binding site 1 out of 8 in 1f3x

Go back to Potassium Binding Sites List in 1f3x
Potassium binding site 1 out of 8 in the S402P Mutant of Rabbit Muscle Pyruvate Kinase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of S402P Mutant of Rabbit Muscle Pyruvate Kinase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K531

b:22.9
occ:1.00
 OD1 A:ASP112 2.5 19.2 1.0
OD1 A:ASN74 2.7 22.1 1.0
O A:THR113 2.8 25.4 1.0
OG A:SER76 3.0 16.1 1.0
CG A:ASP112 3.5 24.6 1.0
CG A:ASN74 3.7 19.6 1.0
C A:THR113 3.7 21.6 1.0
NZ A:LYS269 3.9 16.1 1.0
CB A:SER76 3.9 22.9 1.0
ND2 A:ASN74 4.1 19.9 1.0
O A:ASP112 4.2 18.2 1.0
CA A:LYS114 4.2 21.1 1.0
OD2 A:ASP112 4.3 26.1 1.0
OE1 A:GLU117 4.3 30.0 1.0
N A:SER76 4.3 25.4 1.0
N A:LYS114 4.3 21.9 1.0
C A:ASP112 4.4 18.7 1.0
OG A:SER242 4.4 39.9 1.0
NH2 A:ARG72 4.4 25.1 1.0
CB A:ASP112 4.5 22.1 1.0
CA A:SER76 4.5 25.8 1.0
CB A:SER242 4.6 28.7 1.0
N A:THR113 4.6 20.6 1.0
CA A:THR113 4.7 20.3 1.0
N A:PHE75 4.8 25.1 1.0
O A:LYS114 4.8 20.7 1.0
CB A:ASN74 4.9 21.7 1.0
O3 A:PYR541 4.9 32.3 1.0
C A:LYS114 4.9 22.2 1.0
CA A:ASN74 5.0 23.9 1.0

Potassium binding site 2 out of 8 in 1f3x

Go back to Potassium Binding Sites List in 1f3x
Potassium binding site 2 out of 8 in the S402P Mutant of Rabbit Muscle Pyruvate Kinase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of S402P Mutant of Rabbit Muscle Pyruvate Kinase within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K532

b:34.5
occ:1.00
 OD1 B:ASN74 2.7 25.8 1.0
O B:THR113 2.7 23.9 1.0
OD1 B:ASP112 2.8 31.3 1.0
O B:HOH1034 2.8 18.0 1.0
OG B:SER76 3.0 24.2 1.0
CG B:ASP112 3.6 28.0 1.0
OG B:SER242 3.6 30.2 1.0
CG B:ASN74 3.6 27.2 1.0
C B:THR113 3.7 25.3 1.0
NZ B:LYS269 3.8 29.3 1.0
O B:ASP112 3.8 27.6 1.0
CB B:SER76 3.9 26.7 1.0
ND2 B:ASN74 4.0 25.1 1.0
NH2 B:ARG72 4.2 16.1 1.0
C B:ASP112 4.2 24.0 1.0
CA B:LYS114 4.3 28.0 1.0
CB B:ASP112 4.3 22.3 1.0
OD2 B:ASP112 4.3 23.4 1.0
OE1 B:GLU117 4.3 36.6 1.0
N B:LYS114 4.4 28.5 1.0
N B:SER76 4.4 27.0 1.0
O B:LYS114 4.5 29.6 1.0
CA B:SER76 4.6 28.4 1.0
N B:THR113 4.6 23.9 1.0
CA B:THR113 4.7 26.0 1.0
C B:LYS114 4.8 28.4 1.0
O3 B:PYR542 4.8 20.0 1.0
CB B:SER242 4.8 27.0 1.0
CB B:ASN74 4.8 25.7 1.0
CA B:ASP112 4.9 22.7 1.0
N B:PHE75 5.0 27.1 1.0
CA B:ASN74 5.0 25.7 1.0

Potassium binding site 3 out of 8 in 1f3x

Go back to Potassium Binding Sites List in 1f3x
Potassium binding site 3 out of 8 in the S402P Mutant of Rabbit Muscle Pyruvate Kinase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of S402P Mutant of Rabbit Muscle Pyruvate Kinase within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K533

b:30.9
occ:1.00
 O C:THR113 2.7 27.6 1.0
OD1 C:ASP112 2.7 25.2 1.0
OD1 C:ASN74 2.8 32.4 1.0
OG C:SER76 2.9 24.1 1.0
O C:HOH978 3.4 18.0 1.0
CG C:ASP112 3.6 27.0 1.0
OG C:SER242 3.6 18.8 1.0
CG C:ASN74 3.7 31.5 1.0
CB C:SER76 3.7 27.6 1.0
C C:THR113 3.7 29.2 1.0
O C:ASP112 3.8 28.8 1.0
NZ C:LYS269 3.9 23.1 1.0
ND2 C:ASN74 4.0 30.6 1.0
CA C:LYS114 4.1 28.1 1.0
N C:SER76 4.2 27.8 1.0
OE1 C:GLU117 4.2 34.0 1.0
C C:ASP112 4.2 30.0 1.0
CB C:ASP112 4.3 29.2 1.0
N C:LYS114 4.3 28.9 1.0
CA C:SER76 4.4 29.4 1.0
OD2 C:ASP112 4.4 29.4 1.0
NH2 C:ARG72 4.5 25.2 1.0
O C:LYS114 4.6 27.7 1.0
N C:THR113 4.7 30.4 1.0
C C:LYS114 4.7 28.1 1.0
CB C:SER242 4.8 23.0 1.0
CA C:THR113 4.8 30.9 1.0
CB C:ASN74 4.9 29.9 1.0
O3 C:PYR543 4.9 33.8 1.0
CA C:ASP112 4.9 28.4 1.0
N C:PHE75 4.9 29.1 1.0

Potassium binding site 4 out of 8 in 1f3x

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Potassium binding site 4 out of 8 in the S402P Mutant of Rabbit Muscle Pyruvate Kinase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of S402P Mutant of Rabbit Muscle Pyruvate Kinase within 5.0Å range:
probe atom residue distance (Å) B Occ
D:K534

b:58.4
occ:1.00
 OD1 D:ASN74 2.6 37.8 1.0
OG D:SER76 2.9 51.3 1.0
OD1 D:ASP112 2.9 43.6 1.0
O D:THR113 3.2 48.0 1.0
CG D:ASN74 3.5 39.1 1.0
CG D:ASP112 3.6 44.0 1.0
OG D:SER242 3.7 43.0 1.0
CB D:SER76 3.8 51.9 1.0
ND2 D:ASN74 3.8 37.2 1.0
NZ D:LYS269 3.9 47.0 1.0
OE1 D:GLU117 4.0 53.2 1.0
C D:THR113 4.0 48.8 1.0
NH2 D:ARG72 4.2 34.9 1.0
OD2 D:ASP112 4.3 42.5 1.0
CB D:ASP112 4.3 43.1 1.0
N D:SER76 4.4 50.0 1.0
CA D:LYS114 4.4 49.1 1.0
O D:ASP112 4.5 41.7 1.0
CA D:SER76 4.5 52.1 1.0
N D:LYS114 4.5 49.6 1.0
C D:ASP112 4.6 43.2 1.0
O3 D:PYR544 4.7 45.7 1.0
CB D:ASN74 4.7 41.3 1.0
O D:LYS114 4.7 49.9 1.0
N D:THR113 4.8 44.5 1.0
CB D:SER242 4.9 43.3 1.0
CA D:ASN74 4.9 44.6 1.0
C D:LYS114 4.9 48.6 1.0
N D:PHE75 5.0 45.4 1.0

Potassium binding site 5 out of 8 in 1f3x

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Potassium binding site 5 out of 8 in the S402P Mutant of Rabbit Muscle Pyruvate Kinase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of S402P Mutant of Rabbit Muscle Pyruvate Kinase within 5.0Å range:
probe atom residue distance (Å) B Occ
E:K535

b:22.9
occ:1.00
 OD1 E:ASP112 2.5 19.2 1.0
OD1 E:ASN74 2.7 22.1 1.0
O E:THR113 2.8 25.4 1.0
OG E:SER76 3.0 16.1 1.0
CG E:ASP112 3.6 24.6 1.0
CG E:ASN74 3.7 19.6 1.0
C E:THR113 3.7 21.6 1.0
CB E:SER76 3.8 22.9 1.0
NZ E:LYS269 3.9 16.1 1.0
ND2 E:ASN74 4.1 19.9 1.0
CA E:LYS114 4.2 21.1 1.0
O E:ASP112 4.2 18.2 1.0
OE1 E:GLU117 4.2 30.0 1.0
N E:SER76 4.3 25.4 1.0
OD2 E:ASP112 4.3 26.1 1.0
N E:LYS114 4.4 21.9 1.0
OG E:SER242 4.5 39.9 1.0
NH2 E:ARG72 4.5 25.1 1.0
C E:ASP112 4.5 18.7 1.0
CA E:SER76 4.5 25.8 1.0
CB E:ASP112 4.5 22.1 1.0
CB E:SER242 4.7 28.7 1.0
N E:THR113 4.7 20.6 1.0
CA E:THR113 4.8 20.3 1.0
N E:PHE75 4.8 25.1 1.0
O E:LYS114 4.9 20.7 1.0
CB E:ASN74 4.9 21.7 1.0
O3 E:PYR545 4.9 32.3 1.0
C E:LYS114 4.9 22.2 1.0

Potassium binding site 6 out of 8 in 1f3x

Go back to Potassium Binding Sites List in 1f3x
Potassium binding site 6 out of 8 in the S402P Mutant of Rabbit Muscle Pyruvate Kinase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of S402P Mutant of Rabbit Muscle Pyruvate Kinase within 5.0Å range:
probe atom residue distance (Å) B Occ
F:K536

b:34.5
occ:1.00
 OD1 F:ASN74 2.7 25.8 1.0
O F:THR113 2.7 23.9 1.0
OD1 F:ASP112 2.8 31.3 1.0
O F:HOH2034 2.9 18.0 1.0
OG F:SER76 3.0 24.2 1.0
OG F:SER242 3.6 30.2 1.0
CG F:ASP112 3.6 28.0 1.0
CG F:ASN74 3.6 27.2 1.0
C F:THR113 3.7 25.3 1.0
NZ F:LYS269 3.8 29.3 1.0
O F:ASP112 3.9 27.6 1.0
CB F:SER76 3.9 26.7 1.0
ND2 F:ASN74 4.1 25.1 1.0
NH2 F:ARG72 4.2 16.1 1.0
C F:ASP112 4.2 24.0 1.0
CA F:LYS114 4.3 28.0 1.0
OE1 F:GLU117 4.3 36.6 1.0
OD2 F:ASP112 4.3 23.4 1.0
CB F:ASP112 4.3 22.3 1.0
N F:LYS114 4.4 28.5 1.0
N F:SER76 4.4 27.0 1.0
O F:LYS114 4.4 29.6 1.0
CA F:SER76 4.6 28.4 1.0
N F:THR113 4.6 23.9 1.0
CA F:THR113 4.7 26.0 1.0
C F:LYS114 4.8 28.4 1.0
CB F:SER242 4.8 27.0 1.0
CB F:ASN74 4.8 25.7 1.0
O3 F:PYR546 4.9 20.0 1.0
CA F:ASP112 5.0 22.7 1.0
N F:PHE75 5.0 27.1 1.0

Potassium binding site 7 out of 8 in 1f3x

Go back to Potassium Binding Sites List in 1f3x
Potassium binding site 7 out of 8 in the S402P Mutant of Rabbit Muscle Pyruvate Kinase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 7 of S402P Mutant of Rabbit Muscle Pyruvate Kinase within 5.0Å range:
probe atom residue distance (Å) B Occ
G:K537

b:30.9
occ:1.00
 O G:THR113 2.7 27.6 1.0
OD1 G:ASP112 2.8 25.2 1.0
OG G:SER76 2.9 24.1 1.0
OD1 G:ASN74 2.9 32.4 1.0
O G:HOH1978 3.4 18.0 1.0
CB G:SER76 3.7 27.6 1.0
OG G:SER242 3.7 18.8 1.0
CG G:ASP112 3.7 27.0 1.0
CG G:ASN74 3.7 31.5 1.0
C G:THR113 3.7 29.2 1.0
O G:ASP112 3.8 28.8 1.0
NZ G:LYS269 3.9 23.1 1.0
ND2 G:ASN74 4.0 30.6 1.0
CA G:LYS114 4.1 28.1 1.0
OE1 G:GLU117 4.2 34.0 1.0
N G:SER76 4.2 27.8 1.0
C G:ASP112 4.3 30.0 1.0
N G:LYS114 4.3 28.9 1.0
CB G:ASP112 4.3 29.2 1.0
CA G:SER76 4.4 29.4 1.0
OD2 G:ASP112 4.5 29.4 1.0
O G:LYS114 4.6 27.7 1.0
NH2 G:ARG72 4.6 25.2 1.0
C G:LYS114 4.7 28.1 1.0
N G:THR113 4.7 30.4 1.0
CB G:SER242 4.8 23.0 1.0
CA G:THR113 4.8 30.9 1.0
CB G:ASN74 4.9 29.9 1.0
N G:PHE75 4.9 29.1 1.0
O3 G:PYR547 4.9 33.8 1.0
CA G:ASP112 5.0 28.4 1.0

Potassium binding site 8 out of 8 in 1f3x

Go back to Potassium Binding Sites List in 1f3x
Potassium binding site 8 out of 8 in the S402P Mutant of Rabbit Muscle Pyruvate Kinase


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 8 of S402P Mutant of Rabbit Muscle Pyruvate Kinase within 5.0Å range:
probe atom residue distance (Å) B Occ
H:K538

b:58.4
occ:1.00
 OD1 H:ASN74 2.6 37.8 1.0
OG H:SER76 2.9 51.3 1.0
OD1 H:ASP112 3.0 43.6 1.0
O H:THR113 3.2 48.0 1.0
CG H:ASN74 3.4 39.1 1.0
CG H:ASP112 3.6 44.0 1.0
CB H:SER76 3.7 51.9 1.0
ND2 H:ASN74 3.7 37.2 1.0
OG H:SER242 3.8 43.0 1.0
NZ H:LYS269 4.0 47.0 1.0
OE1 H:GLU117 4.0 53.2 1.0
C H:THR113 4.1 48.8 1.0
NH2 H:ARG72 4.2 34.9 1.0
OD2 H:ASP112 4.2 42.5 1.0
CB H:ASP112 4.3 43.1 1.0
N H:SER76 4.3 50.0 1.0
CA H:LYS114 4.4 49.1 1.0
CA H:SER76 4.5 52.1 1.0
O H:ASP112 4.5 41.7 1.0
N H:LYS114 4.6 49.6 1.0
C H:ASP112 4.6 43.2 1.0
CB H:ASN74 4.6 41.3 1.0
O3 H:PYR548 4.7 45.7 1.0
O H:LYS114 4.7 49.9 1.0
N H:THR113 4.8 44.5 1.0
CA H:ASN74 4.9 44.6 1.0
N H:PHE75 4.9 45.4 1.0
C H:LYS114 5.0 48.6 1.0
CB H:SER242 5.0 43.3 1.0

Reference:

J.O.Wooll, R.H.Friesen, M.A.White, S.J.Watowich, R.O.Fox, J.C.Lee, E.W.Czerwinski. Structural and Functional Linkages Between Subunit Interfaces in Mammalian Pyruvate Kinase. J.Mol.Biol. V. 312 525 2001.
ISSN: ISSN 0022-2836
PubMed: 11563914
DOI: 10.1006/JMBI.2001.4978
Page generated: Mon Aug 12 04:28:11 2024

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