Potassium in PDB 9c0h: Phosphorylated Human NKCC1 in Complex with Bumetanide

The structure of Phosphorylated Human NKCC1 in Complex with Bumetanide also contains other interesting chemical elements:

Sodium	(Na)	2 atoms
Chlorine	(Cl)	2 atoms

The binding sites of Potassium atom in the Phosphorylated Human NKCC1 in Complex with Bumetanide (pdb code 9c0h). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Phosphorylated Human NKCC1 in Complex with Bumetanide, PDB code: 9c0h:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Phosphorylated Human NKCC1 in Complex with Bumetanide


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Phosphorylated Human NKCC1 in Complex with Bumetanide within 5.0Å range: probe atom residue distance (Å) B Occ A:K1301 b:11.6 occ:1.00 O A:ILE299 2.6 37.3 1.0 O4 A:82U1304 2.7 49.7 1.0 O A:PRO496 3.1 36.2 1.0 OH A:TYR383 3.1 33.9 1.0 O A:ASN298 3.1 37.7 1.0 O A:ALA497 3.3 36.4 1.0 C A:ILE299 3.4 29.3 1.0 O A:THR499 3.4 32.3 1.0 C A:ALA497 3.6 32.3 1.0 CA A:ILE299 3.7 25.6 1.0 CA A:ALA497 3.7 32.7 1.0 C17 A:82U1304 3.7 38.0 1.0 OG1 A:THR499 3.9 35.0 1.0 C A:THR499 4.1 31.9 1.0 C A:PRO496 4.1 33.6 1.0 C A:ASN298 4.1 27.0 1.0 O5 A:82U1304 4.2 35.3 1.0 N A:THR499 4.3 32.7 1.0 CZ A:TYR383 4.3 38.8 1.0 N A:ALA497 4.4 33.9 1.0 N A:ILE299 4.4 23.4 1.0 N A:TRP300 4.5 33.3 1.0 N A:GLY301 4.5 43.7 1.0 N A:ALA498 4.6 32.3 1.0 CE1 A:TYR383 4.6 34.1 1.0 CA A:THR499 4.6 31.2 1.0 CG A:82U1304 4.7 37.7 1.0 N A:GLY500 4.8 33.9 1.0 CB A:ILE299 4.9 27.1 1.0 CB A:82U1304 4.9 41.1 1.0 C A:TRP300 4.9 32.6 1.0 C A:ALA498 4.9 31.9 1.0 CB A:ALA497 4.9 33.5 1.0 CA A:GLY301 4.9 41.7 1.0 CG2 A:ILE299 4.9 31.2 1.0 CB A:THR499 4.9 33.4 1.0 OG A:SER614 4.9 23.6 1.0

Potassium binding site 2 out of 2 in the Phosphorylated Human NKCC1 in Complex with Bumetanide


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Phosphorylated Human NKCC1 in Complex with Bumetanide within 5.0Å range: probe atom residue distance (Å) B Occ B:K1301 b:11.0 occ:1.00 O B:ILE299 2.6 35.1 1.0 O4 B:82U1304 2.7 49.7 1.0 O B:ALA497 3.0 35.5 1.0 OH B:TYR383 3.0 34.1 1.0 O B:PRO496 3.1 33.8 1.0 O B:ASN298 3.1 39.3 1.0 O B:THR499 3.3 35.3 1.0 C B:ILE299 3.4 29.1 1.0 C B:ALA497 3.5 30.2 1.0 CA B:ILE299 3.7 26.7 1.0 C17 B:82U1304 3.7 39.0 1.0 CA B:ALA497 3.7 33.8 1.0 OG1 B:THR499 3.8 39.4 1.0 C B:THR499 4.0 32.8 1.0 C B:PRO496 4.1 32.9 1.0 C B:ASN298 4.1 27.8 1.0 O5 B:82U1304 4.2 34.5 1.0 CZ B:TYR383 4.2 39.3 1.0 N B:THR499 4.2 36.1 1.0 N B:ALA497 4.4 37.1 1.0 N B:ILE299 4.4 25.8 1.0 N B:GLY301 4.5 43.7 1.0 N B:TRP300 4.5 33.4 1.0 CA B:THR499 4.6 32.2 1.0 N B:ALA498 4.6 29.9 1.0 CE1 B:TYR383 4.6 32.9 1.0 CG B:82U1304 4.7 37.4 1.0 OG B:SER614 4.7 28.5 1.0 CB B:82U1304 4.8 41.0 1.0 CB B:THR499 4.8 34.1 1.0 CA B:GLY301 4.8 40.8 1.0 N B:GLY500 4.8 34.6 1.0 C B:ALA498 4.8 31.2 1.0 C B:TRP300 4.9 31.0 1.0 CB B:ILE299 4.9 28.4 1.0 CB B:ALA497 4.9 35.3 1.0 CG2 B:ILE299 4.9 29.2 1.0

Y.Zhao, P.Vidossich, B.Forbush, J.Ma, J.Rinehart, M.De Vivo, E.Cao. Structural Basis For Human NKCC1 Inhibition By Loop Diuretic Drugs Embo J. 2025.
ISSN: ESSN 1460-2075
DOI: 10.1038/S44318-025-00368-6