Potassium in PDB 9c0e: Phosphorylated Human NKCC1_K289NA492E in Complex with Furosemide

The structure of Phosphorylated Human NKCC1_K289NA492E in Complex with Furosemide also contains other interesting chemical elements:

Sodium	(Na)	2 atoms
Chlorine	(Cl)	4 atoms
Magnesium	(Mg)	2 atoms

The binding sites of Potassium atom in the Phosphorylated Human NKCC1_K289NA492E in Complex with Furosemide (pdb code 9c0e). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Phosphorylated Human NKCC1_K289NA492E in Complex with Furosemide, PDB code: 9c0e:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Phosphorylated Human NKCC1_K289NA492E in Complex with Furosemide


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Phosphorylated Human NKCC1_K289NA492E in Complex with Furosemide within 5.0Å range: probe atom residue distance (Å) B Occ A:K1303 b:46.7 occ:1.00 O4 A:FUN1302 2.8 61.8 1.0 O A:PRO496 2.9 39.9 1.0 O A:ILE299 2.9 42.9 1.0 OH A:TYR383 3.0 41.5 1.0 O A:ASN298 3.2 35.2 1.0 HN2 A:FUN1302 3.5 56.9 1.0 C A:ILE299 3.6 36.4 1.0 C A:ALA497 3.7 44.4 1.0 O A:ALA497 3.8 51.0 1.0 N A:THR499 3.8 45.0 1.0 CA A:ILE299 3.8 35.6 1.0 C12 A:FUN1302 3.8 54.9 1.0 CA A:ALA497 4.0 41.0 1.0 OG1 A:THR499 4.0 43.4 1.0 C A:PRO496 4.0 42.7 1.0 C A:THR499 4.1 53.3 1.0 C A:ASN298 4.2 30.2 1.0 O5 A:FUN1302 4.2 51.6 1.0 N A:GLY500 4.2 41.8 1.0 N A:ALA498 4.2 41.5 1.0 CZ A:TYR383 4.2 44.7 1.0 CA A:THR499 4.4 44.1 1.0 N2 A:FUN1302 4.5 55.9 1.0 N A:ILE299 4.5 33.8 1.0 O A:THR499 4.5 61.3 1.0 N A:ALA497 4.5 44.5 1.0 CE1 A:TYR383 4.7 50.0 1.0 C A:ALA498 4.7 46.0 1.0 N A:TRP300 4.7 41.6 1.0 CA A:GLY500 4.7 39.7 1.0 CB A:THR499 4.8 41.5 1.0 CA A:ALA498 4.8 40.8 1.0 ND2 A:ASN298 4.8 36.5 1.0 OG A:SER614 4.9 50.8 1.0 N A:GLY301 4.9 46.1 1.0 CB A:ILE299 5.0 31.8 1.0

Potassium binding site 2 out of 2 in the Phosphorylated Human NKCC1_K289NA492E in Complex with Furosemide


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Phosphorylated Human NKCC1_K289NA492E in Complex with Furosemide within 5.0Å range: probe atom residue distance (Å) B Occ B:K1303 b:44.8 occ:1.00 O4 B:FUN1302 2.8 61.9 1.0 O B:ILE299 2.8 42.2 1.0 O B:THR499 2.9 42.5 1.0 O B:PRO496 2.9 37.1 1.0 OH B:TYR383 3.0 43.3 1.0 O B:ASN298 3.2 35.1 1.0 HN2 B:FUN1302 3.4 56.8 1.0 OG1 B:THR499 3.5 44.3 1.0 C B:ILE299 3.6 33.0 1.0 O B:ALA497 3.6 48.7 1.0 C B:THR499 3.7 39.5 1.0 CA B:ILE299 3.8 32.8 1.0 C12 B:FUN1302 3.8 55.6 1.0 C B:ALA497 3.9 43.2 1.0 C B:PRO496 4.0 42.2 1.0 CA B:ALA497 4.0 41.1 1.0 N B:THR499 4.0 41.7 1.0 C B:ASN298 4.2 29.5 1.0 O5 B:FUN1302 4.2 52.6 1.0 CZ B:TYR383 4.3 48.1 1.0 CA B:THR499 4.3 38.1 1.0 N2 B:FUN1302 4.4 55.5 1.0 N B:ILE299 4.5 33.2 1.0 N B:ALA497 4.5 46.6 1.0 CB B:THR499 4.5 38.5 1.0 N B:GLY500 4.6 38.2 1.0 N B:ALA498 4.6 39.3 1.0 N B:TRP300 4.7 40.5 1.0 CE1 B:TYR383 4.7 51.7 1.0 C B:ALA498 4.8 39.5 1.0 N B:GLY301 4.9 48.4 1.0 CA B:GLY500 5.0 34.0 1.0 CB B:ILE299 5.0 30.6 1.0 ND2 B:ASN298 5.0 35.8 1.0

Y.Zhao, P.Vidossich, B.Forbush, J.Ma, J.Rinehart, M.De Vivo, E.Cao. Structural Basis For Human NKCC1 Inhibition By Loop Diuretic Drugs Embo J. 2025.
ISSN: ESSN 1460-2075
DOI: 10.1038/S44318-025-00368-6