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Potassium in PDB 8eh1: Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline

Enzymatic activity of Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline

All present enzymatic activity of Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline:
4.2.1.20;

Protein crystallography data

The structure of Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline, PDB code: 8eh1 was solved by N.J.Porter, P.J.Almhjell, F.H.Arnold, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 38.86 / 2.00
Space group I 4
Cell size a, b, c (Å), α, β, γ (°) 164.862, 164.862, 84.057, 90, 90, 90
R / Rfree (%) 18.8 / 21.9

Potassium Binding Sites:

The binding sites of Potassium atom in the Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline (pdb code 8eh1). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline, PDB code: 8eh1:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 8eh1

Go back to Potassium Binding Sites List in 8eh1
Potassium binding site 1 out of 2 in the Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K403

b:36.8
occ:1.00
 O A:TYR301 2.6 31.1 1.0
O A:GLY303 2.7 28.3 1.0
O A:HOH502 2.8 37.9 1.0
OG A:SER265 2.8 31.4 1.0
O A:ALA263 2.9 29.1 1.0
C A:GLY303 3.7 36.0 1.0
O A:SER228 3.7 35.9 1.0
C A:TYR301 3.7 37.4 1.0
CB A:SER265 3.8 28.1 1.0
O A:HOH552 3.8 34.9 1.0
OG A:SER228 3.8 67.7 1.0
C A:ALA263 4.0 33.6 1.0
N A:GLY303 4.0 31.5 1.0
C A:SER228 4.1 30.7 1.0
CA A:SER228 4.1 39.7 1.0
N A:SER265 4.1 33.6 1.0
C A:PRO302 4.2 33.5 1.0
CB A:ALA263 4.3 31.9 1.0
CA A:GLY303 4.4 29.3 1.0
CA A:PRO302 4.5 26.6 1.0
N A:PRO302 4.5 32.2 1.0
CB A:SER228 4.6 50.1 1.0
O A:LEU299 4.6 33.5 1.0
CB A:TYR301 4.6 28.6 1.0
CA A:SER265 4.6 31.7 1.0
CA A:ALA263 4.6 28.9 1.0
CA A:TYR301 4.6 31.1 1.0
O A:PRO302 4.6 32.5 1.0
N A:VAL304 4.7 30.9 1.0
CD2 A:TYR301 4.7 34.5 1.0
OE2 A:GLU251 4.7 38.6 1.0
N A:TYR301 4.9 33.9 1.0
CB A:VAL304 4.9 28.9 1.0
CA A:VAL304 4.9 29.1 1.0
N A:ALA264 5.0 28.1 1.0
N A:GLY229 5.0 31.6 1.0

Potassium binding site 2 out of 2 in 8eh1

Go back to Potassium Binding Sites List in 8eh1
Potassium binding site 2 out of 2 in the Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Engineered Tyrosine Synthase (TMTYRS1) Derived From T. Maritima Trpb with Ser Bound As the Amino-Acrylate Intermediate and Complexed with 4-Hydroxyquinoline within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K403

b:39.5
occ:1.00
 O B:HOH501 2.6 41.6 1.0
O B:GLY303 2.6 33.2 1.0
O B:TYR301 2.6 33.1 1.0
O B:ALA263 2.8 32.0 1.0
OG B:SER265 2.8 42.7 1.0
O B:SER228 3.7 42.6 1.0
OG B:SER228 3.7 62.7 1.0
C B:GLY303 3.7 34.6 1.0
C B:TYR301 3.8 36.3 1.0
CB B:SER265 3.8 35.8 1.0
O B:HOH524 3.8 37.7 1.0
C B:ALA263 3.9 40.7 1.0
N B:GLY303 4.0 32.5 1.0
C B:SER228 4.0 41.3 1.0
CA B:SER228 4.1 48.3 1.0
N B:SER265 4.1 40.7 1.0
CB B:ALA263 4.2 39.0 1.0
C B:PRO302 4.2 44.8 1.0
OE2 B:GLU251 4.4 54.1 1.0
CA B:GLY303 4.4 26.8 1.0
CB B:SER228 4.5 49.1 1.0
CA B:ALA263 4.5 37.1 1.0
N B:PRO302 4.6 38.2 1.0
CA B:PRO302 4.6 34.2 1.0
O B:LEU299 4.6 41.8 1.0
CA B:SER265 4.6 39.9 1.0
CD2 B:TYR301 4.6 35.2 1.0
CB B:TYR301 4.6 32.7 1.0
CA B:TYR301 4.7 32.5 1.0
N B:VAL304 4.7 34.3 1.0
O B:PRO302 4.7 35.4 1.0
N B:TYR301 4.9 40.6 1.0
CB B:VAL304 4.9 32.4 1.0
N B:ALA264 4.9 35.6 1.0
N B:GLY229 4.9 36.1 1.0
CA B:VAL304 5.0 29.1 1.0

Reference:

N.J.Porter, P.J.Almhjell, F.H.Arnold. Noncanonical L-Tyrosine Synthesis By Evolved Tryptophan Synthases To Be Published.
Page generated: Sat Aug 9 16:46:59 2025

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