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Potassium in PDB 6dpn: Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C, PDB code: 6dpn was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.89 / 1.49
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.501, 37.581, 61.951, 90.00, 96.24, 90.00
R / Rfree (%) 15.9 / 17.9

Other elements in 6dpn:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Iodine (I) 4 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C (pdb code 6dpn). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C, PDB code: 6dpn:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 6dpn

Go back to Potassium Binding Sites List in 6dpn
Potassium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K203

b:35.2
occ:0.35
N A:ARG195 2.6 53.2 0.6
O A:HOH319 2.7 31.8 1.0
O A:ARG195 2.7 58.6 0.6
O A:HOH313 2.8 23.6 0.3
OP2 b:G6 2.9 28.0 1.0
CA A:ARG195 3.2 56.0 0.6
O A:HOH339 3.3 26.1 1.0
OD1 A:ASP192 3.3 34.2 1.0
CB A:ARG195 3.3 56.9 0.6
C A:ARG195 3.4 58.0 0.6
N A:GLY194 3.5 39.3 0.7
O b:HOH101 3.7 52.6 1.0
C A:GLY194 3.7 50.8 0.7
HB2 A:TYR193 3.7 44.4 1.0
H A:TYR193 3.8 33.8 1.0
CA A:GLY194 3.9 45.0 0.7
MG A:MG201 3.9 25.1 1.0
P b:G6 4.0 27.0 1.0
CG A:ARG195 4.0 56.2 0.6
OD2 A:ASP192 4.1 34.0 1.0
CG A:ASP192 4.1 31.4 1.0
C A:TYR193 4.1 43.2 1.0
OP1 b:G6 4.3 29.3 1.0
O5' b:G6 4.3 27.9 1.0
N A:TYR193 4.4 28.1 1.0
CB A:TYR193 4.4 37.0 1.0
CA A:TYR193 4.5 32.5 1.0
HB3 A:TYR193 4.5 44.4 1.0
O A:GLY194 4.8 53.9 0.7
O A:TYR193 4.9 68.1 1.0
CD A:ARG195 4.9 55.1 0.6
OP1 b:C5 5.0 25.7 1.0

Potassium binding site 2 out of 2 in 6dpn

Go back to Potassium Binding Sites List in 6dpn
Potassium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 200 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K101

b:44.9
occ:0.50
O C:HOH211 2.2 22.4 0.5
O6 C:DG5 2.6 29.7 1.0
O B:HOH110 3.0 54.7 1.0
O C:HOH209 3.3 28.6 1.0
O4 C:DT4 3.4 25.6 1.0
C6 C:DG5 3.7 27.1 1.0
N4 B:C2 4.0 29.0 1.0
O C:HOH217 4.0 47.0 1.0
N7 C:DG5 4.1 24.3 1.0
C4 C:DT4 4.2 21.6 1.0
C7 C:DT4 4.3 26.8 1.0
C5 C:DG5 4.3 26.6 1.0
O B:HOH107 4.3 47.3 1.0
O C:HOH202 4.3 35.4 1.0
N6 B:A1 4.3 33.4 1.0
N6 B:A3 4.5 24.9 1.0
C5 C:DT4 4.7 25.1 1.0
N1 C:DG5 4.8 29.6 1.0
O4 C:DT6 5.0 33.5 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Sat Aug 9 10:48:55 2025

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