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Potassium in PDB 6dph: Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C, PDB code: 6dph was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 23.20 / 1.34
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 80.867, 37.063, 61.789, 90.00, 96.33, 90.00
R / Rfree (%) 16 / 19.2

Other elements in 6dph:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Iodine (I) 4 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C (pdb code 6dph). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C, PDB code: 6dph:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in 6dph

Go back to Potassium Binding Sites List in 6dph
Potassium binding site 1 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K203

b:17.2
occ:0.40
MG A:MG201 1.8 16.2 0.6
OD2 A:ASP71 2.4 25.6 0.6
O A:VAL72 2.4 19.9 1.0
OP1 b:C5 2.5 19.2 1.0
OD1 A:ASP71 2.5 18.8 0.4
OD2 A:ASP71 2.7 14.1 0.4
OP2 b:G6 2.8 28.0 1.0
CG A:ASP71 3.0 13.6 0.4
O A:HOH331 3.0 23.1 0.6
CG A:ASP71 3.0 22.1 0.6
OD1 A:ASP71 3.3 22.0 0.6
H A:TYR193 3.3 45.9 1.0
OD1 A:ASP192 3.3 34.2 1.0
HD1 A:TYR193 3.4 37.7 1.0
HB2 A:TYR193 3.6 83.1 1.0
C A:VAL72 3.6 19.2 1.0
O b:HOH103 3.7 34.1 1.0
P b:C5 3.7 18.6 1.0
HA2 A:GLY73 3.7 23.4 1.0
O A:HOH424 3.9 31.4 0.4
C5' b:C5 3.9 18.1 1.0
P b:G6 4.0 21.3 1.0
HA A:ASP192 4.0 30.6 1.0
H A:VAL72 4.0 18.7 1.0
O5' b:C5 4.1 17.9 1.0
N A:TYR193 4.1 38.3 1.0
HG13 A:VAL72 4.1 24.1 1.0
K A:K204 4.2 15.6 0.4
MG A:MG202 4.2 23.8 0.6
HB3 A:ASP71 4.2 27.1 0.6
HG12 A:VAL72 4.3 24.1 1.0
OP1 b:G6 4.3 21.6 1.0
CB A:ASP71 4.3 22.6 0.6
CD1 A:TYR193 4.3 31.4 1.0
N A:VAL72 4.4 15.6 1.0
CB A:ASP71 4.5 12.5 0.4
CB A:TYR193 4.5 69.3 1.0
CA A:GLY73 4.5 19.5 1.0
N A:GLY73 4.5 18.4 1.0
OP2 b:C5 4.5 20.4 1.0
CG A:ASP192 4.5 66.5 1.0
O3' b:C5 4.6 17.2 1.0
CA A:VAL72 4.6 17.6 1.0
CG1 A:VAL72 4.7 20.1 1.0
HA3 A:GLY73 4.8 23.4 1.0
HB3 A:ASP71 4.8 15.0 0.4
CA A:ASP192 4.8 25.5 1.0
CA A:TYR193 4.9 41.7 1.0
CG A:TYR193 4.9 41.9 1.0
HB2 A:ASP71 4.9 27.1 0.6
O3' B:U4 4.9 18.7 1.0
O b:HOH104 4.9 38.4 1.0
HB2 A:ASP71 5.0 15.0 0.4
C4' b:C5 5.0 18.0 1.0

Potassium binding site 2 out of 3 in 6dph

Go back to Potassium Binding Sites List in 6dph
Potassium binding site 2 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K204

b:15.6
occ:0.40
MG A:MG202 0.6 23.8 0.6
OD1 A:ASP71 2.0 22.0 0.6
OD1 A:ASP132 2.5 17.6 1.0
OD2 A:ASP71 2.5 14.1 0.4
OP1 b:C5 2.7 19.2 1.0
OE1 A:GLU109 2.7 28.2 1.0
OD2 A:ASP132 2.8 22.2 1.0
CG A:ASP132 2.9 17.5 1.0
CG A:ASP71 2.9 22.1 0.6
O A:HOH358 2.9 19.7 1.0
O3' B:U4 3.1 18.7 1.0
OE2 A:GLU109 3.3 22.0 1.0
CD A:GLU109 3.4 42.6 1.0
OD2 A:ASP71 3.4 25.6 0.6
O b:HOH103 3.4 34.1 1.0
P b:C5 3.5 18.6 1.0
H A:VAL72 3.5 18.7 1.0
HA A:ASP71 3.6 19.7 1.0
CG A:ASP71 3.7 13.6 0.4
HG12 A:VAL72 3.7 24.1 1.0
HG21 A:ILE189 3.8 28.0 1.0
MG A:MG201 3.9 16.2 0.6
H A:ASP132 4.1 17.2 1.0
CB A:ASP71 4.1 22.6 0.6
K A:K203 4.2 17.2 0.4
HB2 A:ASP71 4.3 27.1 0.6
CB A:ASP132 4.3 17.8 1.0
CA A:ASP71 4.3 17.1 0.6
CA A:ASP71 4.3 16.4 0.4
N A:VAL72 4.4 15.6 1.0
C3' B:U4 4.4 20.8 1.0
CB A:ASP71 4.4 12.5 0.4
HB2 A:ASP71 4.4 15.0 0.4
HG22 A:ILE189 4.4 28.0 1.0
C5' b:C5 4.5 18.1 1.0
O5' b:C5 4.5 17.9 1.0
HB2 A:SER133 4.6 17.5 1.0
O2' B:U4 4.6 19.7 1.0
CG2 A:ILE189 4.6 23.4 1.0
C4' B:U4 4.6 19.5 1.0
HB3 A:ASP132 4.6 21.3 1.0
OD1 A:ASP71 4.7 18.8 0.4
OP2 b:C5 4.7 20.4 1.0
CG1 A:VAL72 4.7 20.1 1.0
N A:ASP132 4.8 14.4 1.0
O A:VAL72 4.9 19.9 1.0
CG A:GLU109 4.9 23.3 1.0
HB3 A:ASP71 4.9 27.1 0.6
HD13 A:ILE189 4.9 23.7 1.0
HB2 A:ASP132 4.9 21.3 1.0
O A:VAL70 4.9 16.1 1.0
CA A:ASP132 4.9 15.5 1.0
C A:ASP71 4.9 14.9 1.0
HG13 A:VAL72 5.0 24.1 1.0

Potassium binding site 3 out of 3 in 6dph

Go back to Potassium Binding Sites List in 6dph
Potassium binding site 3 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K101

b:34.5
occ:0.50
O C:HOH214 2.5 24.1 0.5
O6 C:DG5 2.6 24.4 1.0
O C:HOH213 3.2 27.4 1.0
O4 C:DT4 3.2 21.8 1.0
C6 C:DG5 3.7 23.6 1.0
N4 B:C2 3.9 26.8 1.0
N7 C:DG5 4.1 22.1 1.0
C4 C:DT4 4.1 17.9 1.0
C7 C:DT4 4.2 21.4 1.0
C5 C:DG5 4.2 22.5 1.0
N6 B:A1 4.3 28.0 1.0
O B:HOH107 4.3 44.9 1.0
N6 B:A3 4.4 19.7 1.0
O C:HOH202 4.4 29.8 1.0
C5 C:DT4 4.6 20.5 1.0
O B:HOH103 4.7 53.6 1.0
N1 C:DG5 4.8 24.6 1.0
O4 C:DT6 5.0 29.6 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Sat Aug 9 10:48:33 2025

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