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Potassium in PDB 6dp9: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C, PDB code: 6dp9 was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.77 / 1.40
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.091, 37.976, 61.408, 90.00, 95.96, 90.00
R / Rfree (%) 15.2 / 18.4

Other elements in 6dp9:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C also contains other interesting chemical elements:

Manganese (Mn) 2 atoms
Iodine (I) 4 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C (pdb code 6dp9). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C, PDB code: 6dp9:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in 6dp9

Go back to Potassium Binding Sites List in 6dp9
Potassium binding site 1 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K203

b:22.4
occ:0.35
MN A:MN202 0.3 23.8 0.3
OD1 A:ASP71 2.1 26.4 0.7
OD1 A:ASP132 2.4 25.3 1.0
OE1 A:GLU109 2.6 37.3 1.0
OP1 b:C5 2.7 28.7 1.0
OD2 A:ASP132 2.8 31.2 1.0
OD1 A:ASP71 2.8 16.3 0.3
CG A:ASP132 2.9 24.6 1.0
O A:HOH349 2.9 22.6 1.0
O3' B:U4 3.1 23.6 1.0
CG A:ASP71 3.3 29.0 0.7
OE2 A:GLU109 3.3 24.9 1.0
CD A:GLU109 3.3 28.5 1.0
O A:HOH339 3.5 34.0 1.0
P b:C5 3.5 24.6 1.0
H A:VAL72 3.6 25.4 1.0
HG12 A:VAL72 3.6 30.9 1.0
HA A:ASP71 3.6 24.9 1.0
MN A:MN201 3.8 28.7 0.7
OD2 A:ASP71 4.0 39.4 0.7
CG A:ASP71 4.0 21.8 0.3
H A:ASP132 4.1 22.1 1.0
HG21 A:ILE189 4.1 36.6 1.0
C3' B:U4 4.3 23.8 1.0
CB A:ASP132 4.3 23.3 1.0
CB A:ASP71 4.4 26.2 0.7
C5' b:C5 4.4 20.3 1.0
N A:VAL72 4.4 21.1 1.0
CA A:ASP71 4.4 20.7 0.7
CA A:ASP71 4.4 21.2 0.3
O2' B:U4 4.5 25.5 1.0
O5' b:C5 4.5 20.7 1.0
HB2 A:SER133 4.5 25.6 1.0
CG1 A:VAL72 4.5 25.7 1.0
K A:K204 4.6 20.4 0.3
CB A:ASP71 4.6 19.9 0.3
HB2 A:ASP71 4.6 23.9 0.3
C4' B:U4 4.6 25.9 1.0
HB2 A:ASP71 4.6 31.4 0.7
HB3 A:ASP132 4.6 28.0 1.0
OP2 b:C5 4.7 25.7 1.0
HG22 A:ILE189 4.8 36.6 1.0
CG A:GLU109 4.8 21.8 1.0
N A:ASP132 4.8 18.4 1.0
HG13 A:VAL72 4.8 30.9 1.0
CG2 A:ILE189 4.9 30.5 1.0
O A:VAL72 4.9 23.8 1.0
HB2 A:ASP132 4.9 28.0 1.0
HG11 A:VAL72 5.0 30.9 1.0
CA A:ASP132 5.0 17.3 1.0
OD2 A:ASP71 5.0 28.7 0.3
C A:ASP71 5.0 20.4 1.0

Potassium binding site 2 out of 3 in 6dp9

Go back to Potassium Binding Sites List in 6dp9
Potassium binding site 2 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K204

b:20.4
occ:0.35
MN A:MN201 1.9 28.7 0.7
O A:VAL72 2.5 23.8 1.0
OP2 b:G6 2.7 28.1 1.0
OD2 A:ASP71 2.7 39.4 0.7
OP1 b:C5 2.7 28.7 1.0
O A:HOH355 2.8 55.4 1.0
OD1 A:ASP71 2.9 16.3 0.3
OD2 A:ASP71 2.9 28.7 0.3
CG A:ASP71 3.3 21.8 0.3
CG A:ASP71 3.4 29.0 0.7
H A:TYR193 3.4 49.2 1.0
HB2 A:TYR193 3.4 47.8 1.0
OD1 A:ASP192 3.5 46.3 1.0
OD1 A:ASP71 3.6 26.4 0.7
HD1 A:TYR193 3.6 38.6 1.0
HA2 A:GLY73 3.7 32.0 1.0
C A:VAL72 3.8 21.3 1.0
P b:G6 3.9 24.3 1.0
P b:C5 3.9 24.6 1.0
C5' b:C5 3.9 20.3 1.0
O5' b:C5 4.0 20.7 1.0
O A:HOH339 4.2 34.0 1.0
OP1 b:G6 4.2 22.6 1.0
H A:VAL72 4.2 25.4 1.0
HG13 A:VAL72 4.2 30.9 1.0
HA A:ASP192 4.3 49.0 1.0
N A:TYR193 4.3 41.0 1.0
CB A:TYR193 4.4 39.8 1.0
O3' b:C5 4.4 20.2 1.0
CD1 A:TYR193 4.4 32.2 1.0
CA A:GLY73 4.5 26.7 1.0
HG12 A:VAL72 4.5 30.9 1.0
O b:HOH104 4.5 50.3 1.0
MN A:MN202 4.5 23.8 0.3
N A:GLY73 4.5 23.8 1.0
K A:K203 4.6 22.4 0.3
N A:VAL72 4.6 21.1 1.0
OP2 b:C5 4.6 25.7 1.0
HB3 A:ASP71 4.7 31.4 0.7
CG A:ASP192 4.7 45.0 1.0
CB A:ASP71 4.7 26.2 0.7
HA3 A:GLY73 4.7 32.0 1.0
CA A:VAL72 4.8 20.6 1.0
CG1 A:VAL72 4.8 25.7 1.0
CB A:ASP71 4.8 19.9 0.3
CG A:TYR193 4.9 33.8 1.0
C4' b:C5 4.9 20.0 1.0
C3' b:C5 4.9 20.1 1.0
CA A:TYR193 4.9 43.2 1.0
HB3 A:TYR193 5.0 47.8 1.0

Potassium binding site 3 out of 3 in 6dp9

Go back to Potassium Binding Sites List in 6dp9
Potassium binding site 3 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K101

b:49.5
occ:0.58
O C:HOH210 2.1 28.5 0.6
O6 C:DG5 2.7 28.7 1.0
O4 C:DT4 3.0 25.4 1.0
O B:HOH106 3.2 60.8 1.0
O C:HOH209 3.2 33.1 1.0
C6 C:DG5 3.8 30.9 1.0
C4 C:DT4 3.9 22.2 1.0
C7 C:DT4 4.0 26.2 1.0
O B:HOH108 4.1 56.9 1.0
N7 C:DG5 4.1 25.0 1.0
N4 B:C2 4.2 33.2 1.0
N6 B:A3 4.2 24.4 1.0
C5 C:DG5 4.3 26.6 1.0
C5 C:DT4 4.3 22.3 1.0
N6 B:A1 4.7 32.2 1.0
O C:HOH201 4.7 45.1 1.0
O B:HOH107 4.8 53.2 1.0
N3 C:DT4 4.9 20.9 1.0
N1 C:DG5 5.0 27.5 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Sat Aug 9 10:47:41 2025

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