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Potassium in PDB 6dp0: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C, PDB code: 6dp0 was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.88 / 1.45
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.478, 37.774, 61.811, 90.00, 96.13, 90.00
R / Rfree (%) 19 / 23.6

Other elements in 6dp0:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Iodine (I) 4 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C (pdb code 6dp0). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C, PDB code: 6dp0:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 6dp0

Go back to Potassium Binding Sites List in 6dp0
Potassium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K203

b:47.5
occ:0.35
N A:ARG195 2.7 88.0 0.7
OP2 b:G6 2.8 28.3 1.0
O A:HOH322 2.9 35.0 0.9
O A:HOH335 3.1 26.3 1.0
O A:HOH310 3.2 37.6 0.6
CG A:ARG195 3.2 55.2 0.7
N A:GLY194 3.2 36.1 0.7
HB2 A:TYR193 3.3 42.4 1.0
O A:ARG195 3.3 75.7 0.7
CA A:ARG195 3.4 63.3 0.7
CB A:ARG195 3.5 74.7 0.7
OD1 A:ASP192 3.5 35.1 1.0
H A:TYR193 3.7 36.3 1.0
C A:GLY194 3.7 63.1 0.7
C A:ARG195 3.7 70.0 0.7
P b:G6 3.7 23.4 1.0
CA A:GLY194 3.8 60.2 0.7
C A:TYR193 3.9 39.8 1.0
OP1 b:G6 3.9 25.4 1.0
O b:HOH105 4.0 57.5 1.0
MG A:MG201 4.0 26.3 1.0
CB A:TYR193 4.0 35.3 1.0
OD2 A:ASP192 4.1 33.6 1.0
CG A:ASP192 4.1 31.3 1.0
HB3 A:TYR193 4.2 42.4 1.0
N A:TYR193 4.2 30.3 1.0
CA A:TYR193 4.2 35.1 1.0
O5' b:G6 4.2 29.3 1.0
CD A:ARG195 4.4 42.7 0.7
O A:TYR193 4.7 54.8 1.0
O A:GLY194 4.8 62.6 0.7

Potassium binding site 2 out of 2 in 6dp0

Go back to Potassium Binding Sites List in 6dp0
Potassium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2.5 Mm MG2+ and 75 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K101

b:59.2
occ:0.50
O C:HOH212 2.4 25.0 0.5
O6 C:DG5 2.7 30.4 1.0
O B:HOH113 2.8 62.4 1.0
O4 C:DT4 3.2 25.4 1.0
O C:HOH210 3.3 29.7 1.0
O B:HOH106 3.8 52.3 1.0
C6 C:DG5 3.8 27.1 1.0
C7 C:DT4 4.1 24.8 1.0
N4 B:C2 4.1 29.8 1.0
C4 C:DT4 4.1 22.7 1.0
N7 C:DG5 4.2 23.8 1.0
C5 C:DG5 4.4 23.4 1.0
O1 C:EDO102 4.5 42.4 1.0
N6 B:A3 4.5 23.3 1.0
C5 C:DT4 4.5 23.0 1.0
N6 B:A1 4.6 37.8 1.0
O C:HOH207 4.7 39.0 1.0
N1 C:DG5 5.0 25.3 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Aug 12 15:51:04 2024

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