Potassium in PDB 5ze2: Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C

All present enzymatic activity of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C:
2.3.2.27;

The structure of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C, PDB code: 5ze2 was solved by M.S.Kim, W.Chuenchor, X.Chen, M.Gellert, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.63 / 3.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	132.770, 108.120, 155.740, 90.00, 114.45, 90.00
R / Rfree (%)	20 / 23.7

The structure of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C also contains other interesting chemical elements:

Manganese	(Mn)	4 atoms
Zinc	(Zn)	2 atoms

The binding sites of Potassium atom in the Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C (pdb code 5ze2). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C, PDB code: 5ze2:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C within 5.0Å range: probe atom residue distance (Å) B Occ A:K2006 b:96.0 occ:1.00 OE1 A:GLU649 2.7 76.6 1.0 O2 L:DC19 2.8 90.5 1.0 C2 L:DA18 3.4 95.2 1.0 N3 L:DA18 3.4 95.8 1.0 O4' L:DC19 3.5 95.2 1.0 CD A:GLU649 3.5 77.9 1.0 C2 L:DC19 3.6 90.6 1.0 OE2 A:GLU649 3.7 77.0 1.0 C1' L:DC19 3.7 91.7 1.0 N2 F:DG28 3.7 0.6 1.0 OG A:SER963 3.7 94.4 1.0 CB A:SER963 4.0 82.2 1.0 N1 L:DC19 4.0 89.8 1.0 C4' L:DC19 4.1 86.0 1.0 N1 L:DA18 4.5 93.8 1.0 C4 L:DA18 4.5 93.8 1.0 N3 L:DC19 4.6 91.5 1.0 C2 F:DG28 4.8 90.7 1.0 O3' L:DC19 4.9 83.9 1.0 CG A:GLU649 4.9 68.6 1.0 O4' L:DA20 4.9 84.9 1.0

Potassium binding site 2 out of 2 in the Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C within 5.0Å range: probe atom residue distance (Å) B Occ C:K2005 b:92.1 occ:1.00 OE1 C:GLU649 2.8 0.1 1.0 O4' M:DC19 2.8 98.9 1.0 O2 M:DC19 2.9 0.2 1.0 OG C:SER963 2.9 82.5 1.0 O C:HOH2101 3.0 90.2 1.0 N3 M:DA18 3.4 93.8 1.0 C2 M:DA18 3.5 93.8 1.0 C1' M:DC19 3.6 94.8 1.0 C4' M:DC19 3.6 98.5 1.0 C2 M:DC19 3.6 98.0 1.0 CB C:SER963 3.8 88.3 1.0 N2 G:DG39 3.8 98.8 1.0 CD C:GLU649 3.8 95.1 1.0 N1 M:DC19 4.0 94.6 1.0 OE2 C:GLU649 4.2 95.9 1.0 C4 M:DA18 4.3 89.6 1.0 C5' M:DC19 4.4 95.1 1.0 O C:ASN647 4.4 95.1 1.0 N1 M:DA18 4.5 88.0 1.0 N3 M:DC19 4.7 94.1 1.0 C3' M:DC19 4.8 0.6 1.0 O2 G:DT40 4.9 86.9 1.0 O3' M:DC19 4.9 0.4 1.0 N C:GLU649 4.9 86.3 1.0 C2' M:DC19 4.9 97.8 1.0 C2 G:DG39 5.0 93.7 1.0 N9 M:DA18 5.0 87.8 1.0

M.S.Kim, W.Chuenchor, X.Chen, Y.Cui, X.Zhang, Z.H.Zhou, M.Gellert, W.Yang. Cracking the Dna Code For V(D)J Recombination Mol. Cell V. 70 358 2018.
ISSN: ISSN 1097-4164
PubMed: 29628308
DOI: 10.1016/J.MOLCEL.2018.03.008