Potassium in PDB 5iuk: Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm)

All present enzymatic activity of Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm):
2.7.13.3;

The structure of Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm), PDB code: 5iuk was solved by F.Trajtenberg, J.A.Imelio, N.Larrieux, A.Buschiazzo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.27 / 2.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	87.930, 115.610, 91.590, 90.00, 116.71, 90.00
R / Rfree (%)	19.5 / 23.9

The structure of Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm) also contains other interesting chemical elements:

Magnesium

(Mg)

5 atoms

The binding sites of Potassium atom in the Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm) (pdb code 5iuk). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm), PDB code: 5iuk:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm) within 5.0Å range: probe atom residue distance (Å) B Occ C:K202 b:0.5 occ:1.00 O C:MET27 2.8 95.4 1.0 O C:ASN22 2.9 90.6 1.0 O C:GLU24 3.1 84.5 1.0 C C:ASN22 3.8 86.0 1.0 C C:MET27 4.0 93.8 1.0 CA C:ASN22 4.1 83.3 1.0 C C:GLU24 4.3 86.2 1.0 OD1 C:ASN22 4.4 0.6 1.0 CE C:MET1 4.6 0.3 1.0 CB C:ASN22 4.6 87.2 1.0 CA C:GLU28 4.7 92.1 1.0 N C:GLU28 4.8 90.3 1.0 SD C:MET1 4.8 0.6 1.0 O C:ASP25 4.9 96.2 1.0 N C:LEU23 5.0 77.8 1.0 CG C:ASN22 5.0 0.6 1.0 N C:MET27 5.0 89.5 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of the Desk-Desr Complex in the Phosphotransfer State with High MG2+ (150 Mm) within 5.0Å range: probe atom residue distance (Å) B Occ F:K201 b:0.2 occ:1.00 O F:MET27 2.7 1.0 1.0 O F:ASN22 2.9 0.9 1.0 O F:GLU24 3.0 0.4 1.0 C F:ASN22 3.7 0.1 1.0 C F:MET27 3.9 0.4 1.0 CA F:ASN22 3.9 96.8 1.0 C F:GLU24 4.2 0.9 1.0 OD1 F:ASN22 4.3 0.6 1.0 CB F:ASN22 4.5 1.0 1.0 CA F:GLU28 4.6 0.1 1.0 CE F:MET1 4.6 0.6 1.0 N F:GLU28 4.6 0.1 1.0 N F:LEU23 4.8 99.9 1.0 O F:LEU21 4.8 92.7 1.0 SD F:MET1 4.8 1.0 1.0 N F:GLU24 4.8 0.5 1.0 CG F:ASN22 4.9 0.7 1.0 N F:MET27 4.9 0.4 1.0 CA F:MET27 4.9 0.9 1.0 CB F:GLU28 5.0 0.6 1.0

F.Trajtenberg, J.A.Imelio, M.R.Machado, N.Larrieux, M.A.Marti, G.Obal, A.E.Mechaly, A.Buschiazzo. Regulation of Signaling Directionality Revealed By 3D Snapshots of A Kinase:Regulator Complex in Action. Elife V. 5 2016.
ISSN: ESSN 2050-084X
PubMed: 27938660
DOI: 10.7554/ELIFE.21422