Potassium in PDB 4qgc: Crystal Structure of PKM2 Mutant 4

All present enzymatic activity of Crystal Structure of PKM2 Mutant 4:
2.7.1.40;

The structure of Crystal Structure of PKM2 Mutant 4, PDB code: 4qgc was solved by P.Wang, C.Sun, T.Zhu, Y.Xu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.91 / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	97.095, 70.916, 169.544, 90.00, 100.21, 90.00
R / Rfree (%)	19.7 / 24

The binding sites of Potassium atom in the Crystal Structure of PKM2 Mutant 4 (pdb code 4qgc). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Crystal Structure of PKM2 Mutant 4, PDB code: 4qgc:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Crystal Structure of PKM2 Mutant 4


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of PKM2 Mutant 4 within 5.0Å range: probe atom residue distance (Å) B Occ A:K1001 b:34.0 occ:1.00 OD1 A:ASN75 2.8 23.4 1.0 O A:THR114 2.8 26.0 1.0 OG A:SER77 2.8 26.3 1.0 O A:HOH1102 2.9 19.3 1.0 OD1 A:ASP113 2.9 31.6 1.0 O A:HOH1132 3.0 36.2 1.0 CG A:ASP113 3.8 35.2 1.0 C A:THR114 3.8 20.7 1.0 OG A:SER243 3.8 30.2 1.0 CB A:SER77 3.8 28.4 1.0 O A:ASP113 3.9 22.3 1.0 CG A:ASN75 3.9 30.7 1.0 NZ A:LYS270 4.1 29.1 1.0 CA A:LYS115 4.2 25.2 1.0 N A:SER77 4.3 24.7 1.0 C A:ASP113 4.3 24.1 1.0 OE2 A:GLU118 4.3 37.2 1.0 N A:LYS115 4.4 27.7 1.0 CB A:ASP113 4.4 21.5 1.0 O A:LYS115 4.5 17.9 1.0 NH1 A:ARG73 4.5 25.5 1.0 ND2 A:ASN75 4.5 31.1 1.0 OD2 A:ASP113 4.6 34.8 1.0 CA A:SER77 4.6 30.3 1.0 O3 A:SO41004 4.6 54.4 1.0 N A:THR114 4.7 13.5 1.0 C A:LYS115 4.8 24.9 1.0 N A:PHE76 4.8 25.2 1.0 CA A:THR114 4.8 22.2 1.0 CB A:SER243 4.9 23.7 1.0 CA A:ASP113 5.0 20.7 1.0

Potassium binding site 2 out of 4 in the Crystal Structure of PKM2 Mutant 4


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of PKM2 Mutant 4 within 5.0Å range: probe atom residue distance (Å) B Occ B:K1001 b:37.6 occ:1.00 OD1 B:ASN75 2.8 22.0 1.0 O B:THR114 2.8 25.8 1.0 OG B:SER77 2.8 28.7 1.0 OD1 B:ASP113 3.0 39.5 1.0 CG B:ASP113 3.7 31.8 1.0 C B:THR114 3.8 24.9 1.0 O B:ASP113 3.8 15.4 1.0 CG B:ASN75 3.8 32.1 1.0 OG B:SER243 3.9 42.1 1.0 CB B:SER77 4.0 23.7 1.0 NZ B:LYS270 4.1 40.8 1.0 CA B:LYS115 4.1 34.3 1.0 C B:ASP113 4.2 24.2 1.0 N B:LYS115 4.3 31.4 1.0 CB B:ASP113 4.3 19.7 1.0 N B:SER77 4.4 24.7 1.0 ND2 B:ASN75 4.4 32.4 1.0 OD2 B:ASP113 4.5 37.3 1.0 OE2 B:GLU118 4.6 52.4 1.0 O B:LYS115 4.7 32.5 1.0 N B:THR114 4.7 18.4 1.0 CA B:SER77 4.7 34.9 1.0 NH1 B:ARG73 4.8 20.2 1.0 C B:LYS115 4.8 26.1 1.0 CA B:THR114 4.8 21.2 1.0 N B:PHE76 4.8 20.2 1.0 CA B:ASP113 4.9 20.1 1.0 CB B:SER243 5.0 29.0 1.0 CB B:ASN75 5.0 17.3 1.0

Potassium binding site 3 out of 4 in the Crystal Structure of PKM2 Mutant 4


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of PKM2 Mutant 4 within 5.0Å range: probe atom residue distance (Å) B Occ C:K1001 b:27.7 occ:1.00 OD1 C:ASN75 2.8 18.8 1.0 O C:THR114 2.8 20.7 1.0 OG C:SER77 2.8 32.0 1.0 OD1 C:ASP113 3.0 34.1 1.0 O C:HOH1101 3.0 21.6 1.0 OG C:SER243 3.7 30.4 1.0 CG C:ASP113 3.7 26.0 1.0 C C:THR114 3.8 22.7 1.0 CG C:ASN75 3.9 21.9 1.0 O C:ASP113 3.9 21.6 1.0 CB C:SER77 3.9 29.3 1.0 NZ C:LYS270 4.0 38.3 1.0 CA C:LYS115 4.2 26.2 1.0 C C:ASP113 4.3 19.9 1.0 N C:SER77 4.3 29.8 1.0 CB C:ASP113 4.3 20.0 1.0 N C:LYS115 4.3 25.4 1.0 ND2 C:ASN75 4.4 16.1 1.0 O C:LYS115 4.5 25.5 1.0 OD2 C:ASP113 4.5 27.3 1.0 O C:HOH1163 4.6 39.6 1.0 CA C:SER77 4.6 34.1 1.0 N C:THR114 4.7 17.8 1.0 NH1 C:ARG73 4.7 20.5 1.0 C C:LYS115 4.7 23.4 1.0 CA C:THR114 4.8 22.7 1.0 N C:PHE76 4.8 26.1 1.0 CB C:SER243 4.9 22.5 1.0 CA C:ASP113 4.9 15.6 1.0 OE2 C:GLU118 5.0 38.8 1.0

Potassium binding site 4 out of 4 in the Crystal Structure of PKM2 Mutant 4


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Crystal Structure of PKM2 Mutant 4 within 5.0Å range: probe atom residue distance (Å) B Occ D:K1001 b:39.4 occ:1.00 O D:THR114 2.8 19.3 1.0 OG D:SER77 2.8 34.0 1.0 OD1 D:ASN75 2.8 30.4 1.0 OD1 D:ASP113 2.9 31.3 1.0 O D:HOH1107 2.9 23.3 1.0 CG D:ASP113 3.6 30.7 1.0 C D:THR114 3.8 25.1 1.0 OG D:SER243 3.8 31.5 1.0 CG D:ASN75 3.8 36.0 1.0 NZ D:LYS270 3.9 41.1 1.0 O D:ASP113 4.0 24.1 1.0 CB D:SER77 4.1 35.3 1.0 OD2 D:ASP113 4.2 37.5 1.0 CA D:LYS115 4.3 33.2 1.0 CB D:ASP113 4.3 24.0 1.0 C D:ASP113 4.3 24.1 1.0 ND2 D:ASN75 4.3 30.1 1.0 N D:LYS115 4.4 26.8 1.0 O D:LYS115 4.4 29.4 1.0 N D:SER77 4.5 33.2 1.0 NH1 D:ARG73 4.6 26.3 1.0 N D:THR114 4.7 18.8 1.0 C D:LYS115 4.7 31.3 1.0 CA D:SER77 4.8 33.7 1.0 CA D:THR114 4.8 21.8 1.0 CA D:ASP113 4.9 20.1 1.0 N D:PHE76 5.0 27.3 1.0

P.Wang, C.Sun, T.Zhu, Y.Xu. Structural Insight Into Mechanisms For Dynamic Regulation of PKM2 Protein Cell 2015.
ISSN: ESSN 1674-8018
PubMed: 25645022
DOI: 10.1007/S13238-015-0132-X