Atomistry » Potassium » PDB 3f2y-3gvf » 3fwf
Atomistry »
  Potassium »
    PDB 3f2y-3gvf »
      3fwf »

Potassium in PDB 3fwf: Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form

Enzymatic activity of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form

All present enzymatic activity of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form:
1.14.15.1;

Protein crystallography data

The structure of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form, PDB code: 3fwf was solved by I.Schlichting, K.Von Koenig, C.Aldag, D.Hilvert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 45.64 / 1.83
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 67.520, 61.930, 94.460, 90.00, 90.80, 90.00
R / Rfree (%) 16.2 / 20.7

Other elements in 3fwf:

The structure of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form also contains other interesting chemical elements:

Iron (Fe) 2 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form (pdb code 3fwf). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form, PDB code: 3fwf:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in 3fwf

Go back to Potassium Binding Sites List in 3fwf
Potassium binding site 1 out of 4 in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K515

b:16.5
occ:1.00
 O A:TYR96 2.6 15.3 1.0
O A:GLU84 2.7 17.4 1.0
O A:GLY93 2.8 16.0 1.0
O A:HOH430 2.8 25.9 1.0
O A:HOH467 2.9 35.8 1.0
O A:GLU94 2.9 18.4 1.0
C A:GLU94 3.5 17.5 1.0
C A:TYR96 3.7 15.4 1.0
C A:GLU84 3.8 17.4 1.0
CA A:GLU94 3.8 18.2 1.0
O A:HOH468 3.8 35.2 1.0
C A:GLY93 3.9 17.0 1.0
SG A:CYS85 3.9 27.0 0.5
N A:TYR96 4.1 16.1 1.0
CG A:GLU84 4.2 21.2 1.0
N A:GLU94 4.3 17.4 1.0
CA A:TYR96 4.4 14.8 1.0
CA A:CYS85 4.4 17.1 0.5
CA A:CYS85 4.4 19.5 0.5
N A:CYS85 4.5 18.2 0.5
N A:ALA95 4.5 16.8 1.0
N A:CYS85 4.5 17.1 0.5
C A:ALA95 4.5 16.0 1.0
CB A:TYR96 4.6 15.4 1.0
O A:HOH698 4.7 34.8 1.0
OE2 A:GLU84 4.7 24.9 1.0
N A:ASP97 4.8 13.5 1.0
CA A:GLU84 4.8 17.2 1.0
CB A:CYS85 4.8 19.6 0.5
CB A:CYS85 4.8 16.6 0.5
O A:HOH466 4.9 47.3 1.0
CA A:ASP97 5.0 14.8 1.0

Potassium binding site 2 out of 4 in 3fwf

Go back to Potassium Binding Sites List in 3fwf
Potassium binding site 2 out of 4 in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K515

b:11.8
occ:1.00
 O B:GLU84 2.6 13.2 1.0
O B:TYR96 2.6 11.5 1.0
O B:HOH519 2.8 24.0 1.0
O B:GLY93 2.8 14.0 1.0
O B:GLU94 2.8 11.8 1.0
O B:HOH467 3.2 34.5 1.0
C B:GLU94 3.5 12.2 1.0
C B:TYR96 3.7 10.9 1.0
CA B:GLU94 3.8 12.4 1.0
C B:GLU84 3.8 13.3 1.0
C B:GLY93 3.9 12.9 1.0
SG B:CYS85 3.9 25.6 0.5
N B:TYR96 4.1 11.8 1.0
O B:HOH530 4.3 37.4 1.0
N B:GLU94 4.3 12.2 1.0
O B:HOH537 4.4 37.2 1.0
CA B:TYR96 4.4 10.5 1.0
CA B:CYS85 4.4 11.4 0.5
CG B:GLU84 4.4 24.2 1.0
CA B:CYS85 4.5 15.1 0.5
C B:ALA95 4.5 13.0 1.0
N B:ALA95 4.5 12.0 1.0
N B:CYS85 4.5 13.7 0.5
N B:CYS85 4.5 12.0 0.5
CB B:TYR96 4.6 10.4 1.0
N B:ASP97 4.8 9.9 1.0
CA B:GLU84 4.8 13.6 1.0
CB B:CYS85 4.9 15.0 0.5
CB B:CYS85 4.9 10.4 0.5
O B:ALA95 4.9 12.1 1.0
CA B:ALA95 5.0 12.5 1.0
CA B:ASP97 5.0 11.1 1.0

Potassium binding site 3 out of 4 in 3fwf

Go back to Potassium Binding Sites List in 3fwf
Potassium binding site 3 out of 4 in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K715

b:16.3
occ:1.00
 O B:VAL18 2.6 13.8 1.0
O B:PRO16 2.8 15.8 1.0
O B:HOH8 2.8 17.2 1.0
OE2 B:GLU20 2.9 17.2 1.0
O B:PRO15 2.9 13.9 1.0
C B:PRO16 3.4 14.6 1.0
C B:VAL18 3.6 13.0 1.0
CD B:GLU20 3.6 18.8 1.0
CA B:PRO16 3.7 14.6 1.0
CD2 B:LEU14 3.7 16.9 1.0
C B:PRO15 3.9 14.7 1.0
N B:VAL18 3.9 12.0 1.0
OE1 B:GLU20 3.9 18.5 1.0
CG B:LEU14 4.0 15.6 1.0
O B:HOH425 4.1 25.2 1.0
N B:PRO16 4.2 15.0 1.0
CA B:VAL18 4.3 12.2 1.0
N B:HIS17 4.3 13.2 1.0
O B:HOH721 4.6 39.0 1.0
C B:HIS17 4.6 12.1 1.0
N B:PRO19 4.6 14.0 1.0
N B:GLU20 4.7 14.1 1.0
O B:LEU14 4.8 14.0 1.0
CA B:PRO19 4.8 14.1 1.0
CB B:VAL18 4.8 11.7 1.0
CG B:GLU20 4.8 16.4 1.0
CA B:HIS17 4.9 12.8 1.0
CB B:LEU14 4.9 14.3 1.0
C B:PRO19 5.0 14.3 1.0

Potassium binding site 4 out of 4 in 3fwf

Go back to Potassium Binding Sites List in 3fwf
Potassium binding site 4 out of 4 in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K716

b:24.7
occ:1.00
 O B:HOH541 2.8 20.8 1.0
N B:ARG109 3.1 14.4 1.0
NH1 B:ARG109 3.2 18.8 1.0
C B:PRO106 3.4 17.8 1.0
O B:HOH540 3.4 19.9 1.0
CG B:ARG109 3.5 17.8 1.0
CA B:PRO106 3.5 17.6 1.0
CB B:ARG109 3.6 16.9 1.0
CA B:PRO105 3.6 15.3 1.0
O B:PRO106 3.6 18.4 1.0
N B:GLN108 3.7 14.3 1.0
N B:GLU107 3.9 16.8 0.5
CD B:ARG109 3.9 19.2 1.0
CA B:ARG109 3.9 15.5 1.0
CB B:PRO105 3.9 15.9 1.0
N B:GLU107 3.9 16.7 0.5
C B:GLN108 4.1 13.4 1.0
CZ B:ARG109 4.2 18.1 1.0
CA B:GLN108 4.2 13.3 1.0
CG B:PRO105 4.3 14.7 1.0
C B:GLU107 4.3 15.3 0.5
NE B:ARG109 4.4 16.6 1.0
C B:GLU107 4.4 15.5 0.5
CB B:GLN108 4.4 12.8 1.0
N B:PRO106 4.4 17.8 1.0
N B:PRO105 4.5 14.5 1.0
CA B:GLU107 4.5 16.3 0.5
C B:PRO105 4.6 16.8 1.0
CA B:GLU107 4.6 16.5 0.5
CB B:PRO106 4.6 17.0 1.0
OE1 B:GLN108 4.7 15.7 1.0
N B:GLN110 4.8 14.1 1.0
C B:ARG109 4.9 14.9 1.0
CD B:PRO105 5.0 14.4 1.0

Reference:

C.Aldag, I.A.Gromov, I.Garcia-Rubio, K.Von Koenig, I.Schlichting, B.Jaun, D.Hilvert. Probing the Role of the Proximal Heme Ligand in Cytochrome P450CAM By Recombinant Incorporation of Selenocysteine. Proc.Natl.Acad.Sci.Usa V. 106 5481 2009.
ISSN: ISSN 0027-8424
PubMed: 19293375
DOI: 10.1073/PNAS.0810503106
Page generated: Sat Aug 9 04:57:00 2025

Last articles

Na in 8BFD
Na in 8BB0
Na in 8BBS
Na in 8BBY
Na in 8BAZ
Na in 8BBU
Na in 8B8E
Na in 8BAI
Na in 8B9Z
Na in 8B6E
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy