Potassium in PDB 3ccs: Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation G2482A

The structure of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation G2482A, PDB code: 3ccs was solved by G.Blaha, G.Gurel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.95
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	212.241, 299.185, 575.159, 90.00, 90.00, 90.00
R / Rfree (%)	17.9 / 23.8

The structure of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation G2482A also contains other interesting chemical elements:

Strontium	(Sr)	108 atoms
Magnesium	(Mg)	93 atoms
Cadmium	(Cd)	5 atoms
Chlorine	(Cl)	22 atoms
Sodium	(Na)	75 atoms

The binding sites of Potassium atom in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation G2482A (pdb code 3ccs). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation G2482A, PDB code: 3ccs:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation G2482A


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation G2482A within 5.0Å range: probe atom residue distance (Å) B Occ M:K8402 b:87.0 occ:1.00 O4 0:U172 2.9 48.4 1.0 O M:HOH8823 2.9 39.1 1.0 NH1 M:ARG82 3.0 0.2 1.0 CZ M:ARG82 3.0 0.8 1.0 O 0:HOH7824 3.2 38.2 1.0 NE M:ARG82 3.2 0.6 1.0 O 0:HOH9119 3.3 75.3 1.0 O 0:HOH9022 3.4 49.0 1.0 OP2 0:C162 3.4 36.5 1.0 CD M:ARG82 3.5 0.9 1.0 O4 0:U163 3.6 42.5 1.0 NH2 M:ARG82 3.7 0.1 1.0 O 0:HOH9336 3.8 44.4 1.0 C4 0:U172 3.9 44.8 1.0 N3 0:U172 4.3 43.2 1.0 O M:HOH8890 4.4 64.6 1.0 C4 0:U163 4.6 42.0 1.0 N4 0:C171 4.7 48.1 1.0 P 0:C162 4.7 36.9 1.0 CG M:ARG82 4.7 0.4 1.0 OP2 0:A169 4.9 43.8 1.0 O5' 0:A161 4.9 37.0 1.0 OP1 0:A169 4.9 41.4 1.0

Potassium binding site 2 out of 2 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation G2482A


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation G2482A within 5.0Å range: probe atom residue distance (Å) B Occ 0:K8401 b:0.9 occ:1.00 OP1 0:U2539 2.7 64.3 1.0 O6 0:G2102 2.9 0.5 1.0 O2 0:U2535 3.1 62.5 1.0 C2' 0:U2535 3.1 61.0 1.0 O2' 0:U2535 3.3 61.0 1.0 N6 0:A2482 3.3 59.5 1.0 N7 0:A2482 3.3 58.9 1.0 O4' 0:C2536 3.6 62.5 1.0 C2 0:U2535 3.8 60.1 1.0 C5' 0:C2536 3.8 62.8 1.0 C6 0:G2102 3.8 0.2 1.0 C4' 0:C2536 3.9 63.3 1.0 N7 0:G2102 4.0 0.1 1.0 C5 0:A2482 4.0 58.2 1.0 C6 0:A2482 4.1 58.4 1.0 C1' 0:U2535 4.1 60.0 1.0 P 0:U2539 4.2 66.4 1.0 N1 0:U2535 4.2 59.6 1.0 C5 0:G2102 4.3 0.6 1.0 C3' 0:U2535 4.3 61.2 1.0 C8 0:A2482 4.4 58.4 1.0 C1' 0:C2536 4.6 61.1 1.0 N3 0:U2535 4.6 57.4 1.0 O3' 0:U2535 4.6 63.5 1.0 O3' 0:A2538 4.7 72.2 1.0 O5' 0:C2536 4.7 64.1 1.0 O2 0:C2536 5.0 53.1 1.0

G.Blaha, G.Gurel, S.J.Schroeder, P.B.Moore, T.A.Steitz. Mutations Outside the Anisomycin-Binding Site Can Make Ribosomes Drug-Resistant. J.Mol.Biol. V. 379 505 2008.
ISSN: ISSN 0022-2836
PubMed: 18455733
DOI: 10.1016/J.JMB.2008.03.075