Potassium in PDB 3a3y: Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain

The structure of Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain, PDB code: 3a3y was solved by H.Ogawa, T.Shinoda, F.Cornelius, C.Toyoshima, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.80
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	222.884, 50.720, 163.342, 90.00, 104.63, 90.00
R / Rfree (%)	24.7 / 29.3

The structure of Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain also contains other interesting chemical elements:

Fluorine	(F)	4 atoms
Magnesium	(Mg)	2 atoms

The binding sites of Potassium atom in the Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain (pdb code 3a3y). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain, PDB code: 3a3y:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in the Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain within 5.0Å range: probe atom residue distance (Å) B Occ A:K2003 b:50.0 occ:1.00 O A:HOH5006 2.7 48.4 1.0 O A:THR779 2.9 38.4 1.0 OD2 A:ASP811 3.0 49.6 1.0 OD1 A:ASN783 3.2 47.3 1.0 OG A:SER782 3.3 42.9 1.0 OE2 A:GLU786 3.5 50.1 1.0 N A:ASN783 3.6 44.8 1.0 CG A:ASP811 3.8 47.7 1.0 OD1 A:ASP811 3.8 48.8 1.0 K A:K2004 3.9 72.5 1.0 CG2 A:THR779 4.0 37.8 1.0 C A:SER782 4.0 44.1 1.0 C A:THR779 4.0 38.5 1.0 CG A:ASN783 4.1 46.2 1.0 CB A:SER782 4.1 43.4 1.0 OE1 A:GLU786 4.1 49.4 1.0 CA A:ASN783 4.1 45.5 1.0 O A:HOH5007 4.1 42.6 1.0 CD A:GLU786 4.2 49.8 1.0 CA A:SER782 4.4 43.5 1.0 O A:SER782 4.5 44.2 1.0 CB A:ASN783 4.6 45.6 1.0 OD2 A:ASP815 4.6 40.5 1.0 N A:SER782 4.7 42.9 1.0 CA A:THR779 4.7 38.2 1.0 N A:LEU780 4.9 39.2 1.0 CA A:LEU780 4.9 40.1 1.0 CB A:THR779 5.0 38.1 1.0

Potassium binding site 2 out of 3 in the Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain within 5.0Å range: probe atom residue distance (Å) B Occ A:K2004 b:72.5 occ:1.00 OE2 A:GLU786 3.1 50.1 1.0 OD1 A:ASN783 3.1 47.3 1.0 O A:VAL329 3.3 77.2 1.0 OD2 A:ASP811 3.3 49.6 1.0 OD1 A:ASP811 3.5 48.8 1.0 O A:VAL332 3.5 60.6 1.0 O A:ALA330 3.6 71.3 1.0 CG A:ASP811 3.7 47.7 1.0 OE1 A:GLU334 3.7 56.3 1.0 K A:K2003 3.9 50.0 1.0 C A:ALA330 4.0 71.5 1.0 OE2 A:GLU334 4.0 57.6 1.0 CD A:GLU786 4.1 49.8 1.0 C A:VAL329 4.1 77.2 1.0 CA A:ALA330 4.1 73.2 1.0 CG2 A:ILE807 4.2 46.3 1.0 C A:VAL332 4.2 60.8 1.0 CD A:GLU334 4.3 56.8 1.0 CG A:ASN783 4.3 46.2 1.0 CA A:PRO333 4.4 58.4 1.0 OE1 A:GLU786 4.5 49.4 1.0 N A:ALA330 4.5 75.3 1.0 N A:PRO333 4.6 59.5 1.0 CG1 A:VAL329 4.8 78.2 1.0 CB A:ASP811 4.9 46.4 1.0 N A:ASN331 4.9 69.3 1.0 N A:VAL332 4.9 63.9 1.0

Potassium binding site 3 out of 3 in the Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of the Sodium-Potassium Pump with Bound Potassium and Ouabain within 5.0Å range: probe atom residue distance (Å) B Occ A:K2005 b:76.5 occ:1.00 O A:ALA728 2.9 33.3 1.0 OD1 A:ASP747 3.4 38.2 1.0 O A:LYS726 3.7 35.0 1.0 O A:LEU725 3.8 33.5 1.0 OD2 A:ASP747 3.8 38.9 1.0 CG A:ASP747 4.0 37.4 1.0 C A:LYS726 4.0 34.5 1.0 C A:ALA728 4.0 33.1 1.0 O A:ASP729 4.1 33.2 1.0 C A:ASP729 4.4 32.7 1.0 CA A:LYS726 4.4 34.3 1.0 N A:ALA728 4.6 33.3 1.0 C A:LYS727 4.6 33.8 1.0 N A:LYS727 4.6 34.6 1.0 N A:GLY731 4.6 31.2 1.0 CG1 A:VAL279 4.7 71.1 1.0 C A:LEU725 4.8 33.0 1.0 CA A:ALA728 4.9 33.2 1.0 N A:ILE730 4.9 32.1 1.0 O A:LYS727 4.9 33.6 1.0 CA A:ILE730 4.9 31.3 1.0 CA A:ASP729 4.9 33.0 1.0 N A:ASP729 5.0 33.1 1.0 CA A:LYS727 5.0 34.3 1.0

H.Ogawa, T.Shinoda, F.Cornelius, C.Toyoshima. Crystal Structure of the Sodium-Potassium Pump (Na+,K+-Atpase) with Bound Potassium and Ouabain. Proc.Natl.Acad.Sci.Usa V. 106 13742 2009.
ISSN: ISSN 0027-8424
PubMed: 19666591
DOI: 10.1073/PNAS.0907054106