Potassium in PDB 2x7x: Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754

All present enzymatic activity of Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754:
2.7.13.3;

The structure of Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754, PDB code: 2x7x was solved by E.D.Sonnenburg, H.Zheng, P.Joglekar, S.Higginbottom, S.J.Firbank, D.N.Bolam, J.L.Sonnenburg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.69 / 2.64
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	111.840, 111.840, 115.171, 90.00, 90.00, 120.00
R / Rfree (%)	18.6 / 23.2

The binding sites of Potassium atom in the Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754 (pdb code 2x7x). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754, PDB code: 2x7x:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in the Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754 within 5.0Å range: probe atom residue distance (Å) B Occ A:K1336 b:48.6 occ:1.00 O A:GLU239 2.7 29.2 1.0 O A:MET242 2.8 23.1 1.0 O A:HOH2040 3.4 25.6 1.0 C A:GLU239 3.9 29.0 1.0 C A:MET242 4.0 23.6 1.0 CD2 A:PHE244 4.1 16.7 1.0 CE2 A:PHE244 4.1 15.3 1.0 OD2 A:ASP266 4.1 25.7 1.0 CA A:LYS240 4.3 29.8 1.0 CA A:ILE243 4.6 22.1 1.0 N A:LYS240 4.6 29.4 1.0 OD1 A:ASP266 4.7 25.9 1.0 N A:ILE243 4.8 22.9 1.0 CE1 A:TYR230 4.8 24.6 1.0 C A:LYS240 4.8 28.9 1.0 CG A:ASP266 4.9 25.1 1.0 N A:MET242 4.9 25.6 1.0

Potassium binding site 2 out of 3 in the Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754 within 5.0Å range: probe atom residue distance (Å) B Occ A:K1337 b:42.5 occ:1.00 O A:HOH2007 2.7 12.8 1.0 O A:ILE186 2.7 30.5 1.0 O A:ILE180 2.7 27.6 1.0 O A:PHE183 2.8 31.2 1.0 O A:HOH2021 3.0 31.5 1.0 C A:ILE180 3.8 27.3 1.0 C A:PHE183 3.8 30.7 1.0 C A:ILE186 3.9 30.5 1.0 CA A:PRO184 4.2 31.2 1.0 CA A:SER181 4.2 29.5 1.0 O A:HOH2023 4.4 24.4 1.0 N A:SER181 4.4 28.5 1.0 C A:PRO184 4.4 31.7 1.0 N A:PRO184 4.4 31.0 1.0 CD1 A:ILE180 4.5 24.4 1.0 O A:SER181 4.5 30.4 1.0 O A:HOH2019 4.6 26.5 1.0 O A:PRO184 4.6 32.0 1.0 C A:SER181 4.6 30.1 1.0 N A:ILE186 4.6 30.9 1.0 CG1 A:ILE180 4.6 25.4 1.0 N A:PHE183 4.7 30.5 1.0 CA A:ILE186 4.7 30.6 1.0 CB A:ILE180 4.7 25.5 1.0 CA A:PHE183 4.8 30.2 1.0 CB A:ILE186 4.8 30.6 1.0 CA A:ILE180 4.8 26.1 1.0 N A:LYS187 4.9 30.0 1.0 N A:ASP185 4.9 31.9 1.0 CA A:LYS187 4.9 30.0 1.0

Potassium binding site 3 out of 3 in the Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Fructose Binding Periplasmic Domain of Hybrid Two Component System BT1754 within 5.0Å range: probe atom residue distance (Å) B Occ B:K1335 b:65.7 occ:1.00 O B:MET242 2.9 28.6 1.0 O B:GLU239 3.0 34.5 1.0 O B:HOH2044 3.3 23.1 1.0 OD2 B:ASP266 3.8 29.1 1.0 C B:GLU239 4.1 34.1 1.0 C B:MET242 4.1 27.9 1.0 CD2 B:PHE244 4.2 19.7 1.0 OD1 B:ASP266 4.3 30.6 1.0 CE2 B:PHE244 4.4 21.2 1.0 CA B:LYS240 4.5 33.8 1.0 CG B:ASP266 4.5 29.6 1.0 CA B:ILE243 4.6 25.6 1.0 N B:LYS240 4.7 34.0 1.0 CE1 B:TYR230 4.8 29.3 1.0 N B:ILE243 4.8 26.8 1.0 C B:LYS240 4.9 33.1 1.0 O B:LYS240 5.0 33.2 1.0

E.D.Sonnenburg, H.Zheng, P.Joglekar, S.K.Higginbottom, S.J.Firbank, D.N.Bolam, J.L.Sonnenburg. Specificity of Polysaccharide Use in Intestinal Bacteroides Species Determines Diet-Induced Microbiota Alterations. Cell(Cambridge,Mass.) V. 141 1241 2010.
ISSN: ISSN 0092-8674
PubMed: 20603004
DOI: 10.1016/J.CELL.2010.05.005