Potassium in PDB 2frz: Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A)

All present enzymatic activity of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A):
1.14.15.1;

The structure of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A), PDB code: 2frz was solved by Z.Rao, L.L.Wong, F.Xu, S.G.Bell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	66.816, 62.114, 94.940, 90.00, 90.46, 90.00
R / Rfree (%)	19 / 24.3

The structure of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A) also contains other interesting chemical elements:

Iron

(Fe)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A) (pdb code 2frz). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A), PDB code: 2frz:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A) within 5.0Å range: probe atom residue distance (Å) B Occ A:K1418 b:13.2 occ:1.00 O A:PHE96 2.8 14.3 1.0 O A:GLU84 2.9 15.8 1.0 O A:GLU94 3.0 13.6 1.0 O A:GLY93 3.0 16.6 1.0 OE2 A:GLU84 3.5 35.5 1.0 C A:GLU94 3.5 15.2 1.0 CA A:GLU94 3.8 17.0 1.0 C A:PHE96 3.9 13.0 1.0 O A:HOH1651 3.9 35.1 1.0 C A:GLU84 4.0 16.9 1.0 C A:GLY93 4.0 16.2 1.0 CD A:GLU84 4.2 33.9 1.0 N A:PHE96 4.2 14.1 1.0 N A:GLU94 4.4 16.2 1.0 CA A:PHE96 4.5 13.2 1.0 N A:ALA95 4.5 15.1 1.0 OE1 A:GLU84 4.5 35.7 1.0 C A:ALA95 4.6 14.6 1.0 CB A:PHE96 4.6 12.5 1.0 CA A:CYS85 4.7 14.3 1.0 N A:CYS85 4.8 14.2 1.0 O A:HOH1608 4.8 29.2 1.0 N A:ASP97 4.9 13.1 1.0 O A:ALA95 5.0 15.9 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Cytochrome P450CAM Mutant (F87W/Y96F/V247L/C334A) within 5.0Å range: probe atom residue distance (Å) B Occ B:K2418 b:17.9 occ:1.00 O B:GLU84 2.8 25.6 1.0 O B:PHE96 2.9 20.7 1.0 O B:GLY93 3.0 24.1 1.0 O B:GLU94 3.1 22.9 1.0 C B:GLU94 3.6 23.1 1.0 CA B:GLU94 3.8 25.5 1.0 C B:PHE96 3.8 19.4 1.0 C B:GLU84 3.9 25.3 1.0 C B:GLY93 4.1 24.7 1.0 N B:PHE96 4.2 21.2 1.0 CA B:PHE96 4.4 19.7 1.0 N B:GLU94 4.4 24.5 1.0 N B:ALA95 4.5 22.0 1.0 CA B:CYS85 4.5 23.4 1.0 C B:ALA95 4.6 20.3 1.0 CG B:GLU84 4.6 38.4 1.0 CB B:PHE96 4.6 20.0 1.0 N B:CYS85 4.6 24.5 1.0 N B:ASP97 4.8 18.7 1.0 CB B:CYS85 4.9 24.4 1.0 CA B:GLU84 4.9 25.7 1.0

F.Xu, S.G.Bell, G.Taylor, W.Ji, N.Bishop, J.C.Green, Z.Rao, L.L.Wong. Pentachlorobenzene Oxidation By Engineered Cytochrome P450CAM: Substrate Binding and the Mechanism of Aromatic C-H Bond Oxidation To Be Published.