Potassium in PDB 9ewd: Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex

All present enzymatic activity of Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex:
2.7.7.7;

The structure of Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex, PDB code: 9ewd was solved by A.Nourisson, A.Haouz, S.Missoury, M.Delarue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.89 / 2.12
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.4, 62.47, 139.57, 90, 90, 90
R / Rfree (%)	20.2 / 24.5

The structure of Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex also contains other interesting chemical elements:

Sodium	(Na)	2 atoms
Calcium	(Ca)	1 atom

The binding sites of Potassium atom in the Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex (pdb code 9ewd). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex, PDB code: 9ewd:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:K607 b:77.0 occ:1.00 O A:ILE302 2.3 78.2 1.0 O D:HOH103 2.5 61.1 1.0 O A:CYS300 2.7 86.6 1.0 O A:HOH768 2.8 77.3 1.0 O A:ILE305 3.0 74.2 1.0 OP1 D:DC3 3.1 63.5 1.0 C A:ILE302 3.1 80.5 1.0 C A:CYS300 3.6 86.1 1.0 CA A:PRO303 3.7 78.7 1.0 N A:PRO303 3.7 79.9 1.0 N A:ILE302 3.8 86.7 1.0 N A:ILE305 3.9 70.2 1.0 N A:GLY304 4.0 69.3 1.0 C A:ILE305 4.0 66.0 1.0 C A:SER301 4.0 86.6 1.0 P D:DC3 4.1 67.7 1.0 CA A:ILE302 4.1 83.6 1.0 OP2 D:DC3 4.1 60.5 1.0 C A:PRO303 4.2 73.6 1.0 CA A:CYS300 4.3 83.2 1.0 O A:SER301 4.4 90.1 1.0 CA A:ILE305 4.5 66.8 1.0 N A:SER301 4.5 84.8 1.0 CA A:SER301 4.6 84.4 1.0 O A:ALA299 4.7 80.9 1.0 CB A:ILE305 4.9 69.1 1.0 C A:GLY304 4.9 66.4 1.0

Potassium binding site 2 out of 2 in the Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Dna Polymerase Lambda I493K E529D, Tmp:at CA2+ Ground State Ternary Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:K608 b:72.7 occ:1.00 O A:HOH834 2.7 58.3 1.0 O A:ALA367 2.8 39.4 1.0 O A:ARG364 2.8 39.1 1.0 O A:ILE363 3.3 34.6 1.0 C A:ARG364 3.6 40.1 1.0 N A:ALA367 3.8 36.1 1.0 C A:ALA367 3.8 36.9 1.0 C A:SER365 4.1 38.4 1.0 N A:GLN366 4.1 39.1 1.0 O A:HOH799 4.1 50.9 1.0 CD1 A:LEU369 4.2 44.6 1.0 C A:GLN366 4.3 43.2 1.0 O A:SER365 4.3 44.0 1.0 CA A:ALA367 4.3 38.2 1.0 N A:SER365 4.4 36.6 1.0 CA A:ARG364 4.4 35.8 1.0 C A:ILE363 4.4 36.5 1.0 CG A:LEU369 4.4 38.5 1.0 CA A:SER365 4.6 43.5 1.0 CA A:GLN366 4.6 38.1 1.0 O A:HOH828 4.8 53.6 1.0 O A:HOH850 4.8 62.0 1.0 CB A:ALA367 4.8 34.4 1.0 O A:HOH848 4.9 45.0 1.0 N A:ARG364 4.9 30.4 1.0 O A:GLN366 5.0 45.2 1.0

A.Nourisson, A.Haouz, S.Missoury, M.Delarue. Dna Polymerase Lambda I493K E529D, Ttp:at CA2+ Ground State Ternary Complex To Be Published.