Potassium in PDB 8rsn: Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1

All present enzymatic activity of Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1:
3.1.3.84;

The structure of Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1, PDB code: 8rsn was solved by A.Ariza, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	99.86 / 2.22
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	61.739, 135.089, 147.625, 90, 90, 90
R / Rfree (%)	20.3 / 25.3

The structure of Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1 also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Potassium atom in the Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1 (pdb code 8rsn). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1, PDB code: 8rsn:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:K611 b:54.3 occ:1.00 O A:HIS185 2.8 39.3 1.0 O A:LEU187 2.8 28.2 1.0 O A:HOH773 2.8 52.5 1.0 O A:ALA189 2.8 36.8 1.0 N A:HIS185 3.7 35.7 1.0 C A:HIS185 3.7 37.2 1.0 C A:ALA189 3.8 35.0 1.0 C A:LEU187 3.8 30.3 1.0 C A:GLY184 3.9 36.0 1.0 CA A:GLY184 4.2 37.8 1.0 CA A:HIS185 4.2 36.6 1.0 CA A:SER190 4.3 32.0 1.0 N A:LEU187 4.4 36.9 1.0 C A:PHE188 4.4 35.7 1.0 O A:GLY184 4.4 31.6 1.0 N A:SER190 4.4 33.3 1.0 N A:ALA189 4.5 33.7 1.0 O A:PHE188 4.5 36.3 1.0 CA A:LEU187 4.6 35.0 1.0 O A:SER190 4.6 33.9 1.0 N A:GLY184 4.7 34.9 1.0 N A:PHE188 4.7 33.1 1.0 N A:ALA186 4.8 35.7 1.0 CA A:ALA189 4.8 34.0 1.0 C A:ALA186 4.8 42.9 1.0 C A:SER190 4.8 29.5 1.0 CA A:PHE188 4.8 34.0 1.0 CB A:LEU187 4.9 35.9 1.0

Potassium binding site 2 out of 2 in the Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Macrodomain-Fused Sirtm (MFS1) From Fusarium Oxysporum F. Sp. Cubense Race 1 within 5.0Å range: probe atom residue distance (Å) B Occ B:K610 b:75.7 occ:1.00 O B:ALA189 2.8 53.9 1.0 O B:HIS185 2.8 61.9 1.0 O B:LEU187 2.9 52.0 1.0 O B:HOH778 2.9 67.6 1.0 N B:HIS185 3.6 56.6 1.0 C B:ALA189 3.7 49.4 1.0 C B:HIS185 3.7 59.4 1.0 C B:GLY184 3.8 56.9 1.0 C B:LEU187 3.9 50.0 1.0 CA B:GLY184 4.1 57.3 1.0 CA B:HIS185 4.1 54.9 1.0 CA B:SER190 4.1 51.5 1.0 N B:SER190 4.3 50.8 1.0 O B:GLY184 4.3 57.4 1.0 N B:LEU187 4.3 52.4 1.0 O B:SER190 4.3 52.6 1.0 C B:PHE188 4.4 53.0 1.0 O B:PHE188 4.5 55.6 1.0 N B:GLY184 4.5 62.9 1.0 N B:ALA189 4.5 51.6 1.0 C B:SER190 4.5 53.4 1.0 CA B:LEU187 4.6 50.5 1.0 CA B:ALA189 4.8 51.9 1.0 CB B:LEU187 4.8 51.7 1.0 N B:ALA186 4.8 53.5 1.0 N B:PHE188 4.8 52.0 1.0 C B:ALA186 4.8 51.6 1.0 CA B:PHE188 4.9 51.2 1.0

A.Ariza, Q.Liu, N.Cowleson, I.Ahel, D.V.Filippov, J.G.M.Rack. Evolutionary and Molecular Basis of Adp-Ribosylation Reversal By Zinc-Dependent Macrodomains To Be Published.