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Potassium in PDB 8fol: The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form

Enzymatic activity of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form

All present enzymatic activity of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form:
1.97.1.4;

Protein crystallography data

The structure of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form, PDB code: 8fol was solved by J.D.Moody, A.J.Saxton, A.Galambas, C.M.Lawrence, J.B.Broderick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 35.37 / 2.65
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 49.653, 59.011, 96.792, 90, 90, 90
R / Rfree (%) 19.5 / 21.4

Other elements in 8fol:

The structure of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form also contains other interesting chemical elements:

Iron (Fe) 4 atoms
Chlorine (Cl) 3 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form (pdb code 8fol). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total only one binding site of Potassium was determined in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form, PDB code: 8fol:

Potassium binding site 1 out of 1 in 8fol

Go back to Potassium Binding Sites List in 8fol
Potassium binding site 1 out of 1 in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, Alternate Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K303

b:75.3
occ:1.00
 OD1 A:ASP104 2.7 75.9 1.0
OD1 A:ASP129 2.8 63.3 1.0
OXT A:SAM302 2.9 67.7 1.0
O A:MET127 3.1 39.6 1.0
CG A:ASP104 3.3 62.5 1.0
O A:THR105 3.4 36.2 1.0
N A:THR105 3.5 40.0 1.0
CG A:ASP129 3.6 53.8 1.0
OD2 A:ASP104 3.7 68.0 1.0
C A:SAM302 3.7 63.1 1.0
C A:ASP104 3.8 48.9 1.0
C A:THR105 3.9 44.2 1.0
CB A:ASP129 3.9 45.1 1.0
N A:ASP129 4.0 48.0 1.0
O3' A:SAM302 4.0 63.5 1.0
C A:MET127 4.0 42.0 1.0
CB A:SAM302 4.0 63.1 1.0
CA A:ASP104 4.1 47.9 1.0
C3' A:SAM302 4.2 68.6 1.0
CA A:SAM302 4.2 55.9 1.0
CA A:THR105 4.3 47.3 1.0
CB A:ASP104 4.3 49.1 1.0
C A:LEU128 4.4 36.1 1.0
CA A:ASP129 4.4 41.8 1.0
O A:ASP104 4.5 52.3 1.0
OD2 A:ASP129 4.6 55.0 1.0
CB A:MET127 4.6 31.1 1.0
CA A:LEU128 4.6 33.6 1.0
N A:LEU128 4.6 39.5 1.0
O A:SAM302 4.7 58.8 1.0
N A:ASN106 4.7 46.6 1.0
CA A:MET127 4.9 37.9 1.0
CG A:ARG166 5.0 51.7 1.0
C4' A:SAM302 5.0 63.8 1.0

Reference:

J.D.Moody, S.Hill, M.N.Lundahl, A.J.Saxton, A.Galambas, W.E.Broderick, C.M.Lawrence, J.B.Broderick. Computational Engineering of Previously Crystallized Pyruvate Formate-Lyase Activating Enzyme Reveals Insights Into Sam Binding and Reductive Cleavage. J.Biol.Chem. 04791 2023.
ISSN: ESSN 1083-351X
PubMed: 37156396
DOI: 10.1016/J.JBC.2023.104791
Page generated: Sat Aug 9 16:52:58 2025

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