Potassium in PDB 8cu4: Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences

The structure of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences, PDB code: 8cu4 was solved by B.Lee, K.I.White, M.A.Socolich, M.A.Klureza, R.Henning, V.Srajer, R.Ranganathan, D.Hekstra, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.59 / 2.01
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	68.816, 68.816, 90.365, 90, 90, 90
R / Rfree (%)	34.3 / 37.2

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Potassium atom in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences (pdb code 8cu4). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 12 binding sites of Potassium where determined in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences, PDB code: 8cu4:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Potassium binding site 1 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ A:K211 b:7.3 occ:0.19 O A:TYR66 2.6 14.5 1.0 O A:GLY65 3.2 15.1 1.0 C A:TYR66 3.4 7.7 1.0 K A:K212 3.6 12.9 0.1 HA A:TYR66 3.7 6.3 1.0 HA3 A:GLY67 3.8 22.3 1.0 CA A:TYR66 4.1 5.3 1.0 N A:GLY67 4.3 13.3 1.0 C A:GLY65 4.3 24.2 1.0 CA A:GLY67 4.5 18.6 1.0 N A:TYR66 4.7 6.6 1.0 K A:K215 4.8 35.0 0.2

Potassium binding site 2 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ A:K212 b:12.9 occ:0.12 O A:VAL64 2.8 10.9 1.0 O A:GLY65 2.8 15.1 1.0 K A:K213 2.9 14.7 0.2 C A:GLY65 3.5 24.2 1.0 HA3 A:GLY65 3.6 13.2 1.0 K A:K211 3.6 7.3 0.2 C A:VAL64 3.9 11.1 1.0 CA A:GLY65 4.0 11.0 1.0 HA A:TYR66 4.3 6.3 1.0 N A:GLY65 4.4 16.2 1.0 N A:TYR66 4.5 6.6 1.0 O A:THR63 4.8 15.7 1.0 CA A:TYR66 4.8 5.3 1.0 HA2 A:GLY65 4.9 13.2 1.0

Potassium binding site 3 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ A:K213 b:14.7 occ:0.25 O A:THR63 2.6 15.7 1.0 O A:VAL64 2.8 10.9 1.0 K A:K212 2.9 12.9 0.1 K A:K214 3.5 16.8 0.2 C A:VAL64 3.5 11.1 1.0 HA A:VAL64 3.7 4.9 1.0 C A:THR63 3.8 11.5 1.0 CA A:VAL64 4.1 4.1 1.0 HA3 A:GLY65 4.2 13.2 1.0 N A:GLY65 4.4 16.2 1.0 N A:VAL64 4.4 11.3 1.0 HB A:THR63 4.5 12.5 1.0 CA A:GLY65 4.7 11.0 1.0 O A:GLY65 5.0 15.1 1.0 CA A:THR63 5.0 10.5 1.0

Potassium binding site 4 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ A:K214 b:16.8 occ:0.20 OG1 A:THR63 2.8 10.3 1.0 HB A:THR63 3.1 12.5 1.0 O A:THR63 3.1 15.7 1.0 K A:K213 3.5 14.7 0.2 CB A:THR63 3.5 10.5 1.0 C A:THR63 3.9 11.5 1.0 CA A:THR63 4.3 10.5 1.0 HA A:VAL64 4.5 4.9 1.0 HG23 A:THR63 4.5 19.1 1.0 CG2 A:THR63 4.6 15.9 1.0 N A:VAL64 4.8 11.3 1.0 HA A:THR63 4.9 12.6 1.0 O A:THR62 4.9 13.2 1.0

Potassium binding site 5 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ A:K215 b:35.0 occ:0.25 O A:GLY67 4.6 22.6 1.0 O A:TYR66 4.6 14.5 1.0 HA3 A:GLY67 4.7 22.3 1.0 O A:HOH321 4.8 43.2 1.0 K A:K211 4.8 7.3 0.2 K A:K216 4.8 38.4 0.2

Potassium binding site 6 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ A:K216 b:38.4 occ:0.25 O A:HOH327 4.3 37.8 1.0 O A:HOH321 4.5 43.2 1.0 K A:K215 4.8 35.0 0.2

Potassium binding site 7 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 7 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ B:K212 b:13.3 occ:0.21 O B:TYR66 2.6 16.6 1.0 HA3 B:GLY67 3.5 9.9 1.0 C B:TYR66 3.5 18.6 1.0 O B:GLY65 3.8 10.4 1.0 HA B:TYR66 4.1 22.8 1.0 N B:GLY67 4.3 14.1 1.0 CA B:GLY67 4.3 8.3 1.0 CA B:TYR66 4.4 19.0 1.0 K B:K213 4.6 10.4 0.2 C B:GLY65 4.9 9.3 1.0 HA2 B:GLY67 4.9 9.9 1.0

Potassium binding site 8 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 8 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ B:K213 b:10.4 occ:0.25 O B:VAL64 2.7 18.5 1.0 O B:GLY65 2.8 10.4 1.0 K B:K214 3.0 17.2 0.2 C B:GLY65 3.5 9.3 1.0 HA3 B:GLY65 3.5 13.1 1.0 C B:VAL64 3.8 17.4 1.0 CA B:GLY65 3.9 10.9 1.0 N B:GLY65 4.3 15.2 1.0 HA B:TYR66 4.4 22.8 1.0 N B:TYR66 4.4 21.9 1.0 K B:K212 4.6 13.3 0.2 HA2 B:GLY65 4.8 13.1 1.0 CA B:TYR66 4.8 19.0 1.0 HA B:VAL64 5.0 16.3 1.0

Potassium binding site 9 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 9 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ B:K214 b:17.2 occ:0.25 O B:THR63 2.7 29.9 1.0 O B:VAL64 2.7 18.5 1.0 K B:K213 3.0 10.4 0.2 HA B:VAL64 3.4 16.3 1.0 C B:VAL64 3.4 17.4 1.0 C B:THR63 3.8 25.5 1.0 K B:K215 3.8 25.1 0.2 CA B:VAL64 3.9 13.6 1.0 HA3 B:GLY65 4.3 13.1 1.0 N B:VAL64 4.4 11.8 1.0 N B:GLY65 4.4 15.2 1.0 HB B:THR63 4.5 23.5 1.0 CA B:GLY65 4.8 10.9 1.0 CA B:THR63 5.0 10.4 1.0

Potassium binding site 10 out of 12 in the Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 10 of Structure of A K+ Selective Nak Mutant (NAK2K, Laue Diffraction) in the Presence of An Electric Field of ~0.8MV/Cm Along the Crystallographic Z Axis, 1US, with Eightfold Extrapolation of Structure Factor Differences within 5.0Å range: probe atom residue distance (Å) B Occ B:K215 b:25.1 occ:0.25 OG1 B:THR63 2.8 16.6 1.0 HB B:THR63 3.0 23.5 1.0 O B:THR63 3.2 29.9 1.0 CB B:THR63 3.4 19.6 1.0 K B:K214 3.8 17.2 0.2 C B:THR63 4.0 25.5 1.0 HG21 B:THR63 4.4 45.9 1.0 CA B:THR63 4.4 10.4 1.0 CG2 B:THR63 4.5 38.3 1.0 HA B:VAL64 4.6 16.3 1.0 O B:THR62 4.9 15.3 1.0 HA B:THR63 4.9 12.4 1.0 N B:VAL64 5.0 11.8 1.0

B.Lee, K.I.White, M.A.Socolich, M.A.Klureza, R.Henning, V.Srajer, R.Ranganathan, D.Hekstra. Direct Visualization of Electric Field-Stimulated Ion Conduction in A Potassium Channel To Be Published.