Potassium in PDB 8b2s: GH24 Family Muramidase From Trichophaea Saccata with An SH3-Like Cell Wall Binding Domain

The structure of GH24 Family Muramidase From Trichophaea Saccata with An SH3-Like Cell Wall Binding Domain, PDB code: 8b2s was solved by O.V.Moroz, E.Blagova, A.A.Lebedev, L.K.Skov, R.A.Pache, K.M.Schnorr, L.Kiemer, S.Nymand-Grarup, L.Ming, L.Ye, M.Klausen, M.T.Cohn, E.G.W.Schmidt, G.J.Davies, K.S.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.58 / 1.94
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	99.438, 99.438, 133.254, 90, 90, 120
R / Rfree (%)	16.8 / 20.1

The binding sites of Potassium atom in the GH24 Family Muramidase From Trichophaea Saccata with An SH3-Like Cell Wall Binding Domain (pdb code 8b2s). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the GH24 Family Muramidase From Trichophaea Saccata with An SH3-Like Cell Wall Binding Domain, PDB code: 8b2s:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the GH24 Family Muramidase From Trichophaea Saccata with An SH3-Like Cell Wall Binding Domain


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of GH24 Family Muramidase From Trichophaea Saccata with An SH3-Like Cell Wall Binding Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:K301 b:69.6 occ:1.00 O A:HOH540 2.4 44.7 1.0 O A:HOH551 2.5 49.7 1.0 O A:VAL158 2.7 45.5 1.0 O A:SER155 2.7 44.6 1.0 OD1 A:ASP160 2.8 54.5 1.0 O A:ASN156 2.8 45.9 1.0 O A:HOH402 3.1 53.1 1.0 C A:ASN156 3.5 40.6 1.0 C A:VAL158 3.7 43.7 1.0 C A:SER155 3.8 45.9 1.0 CG A:ASP160 3.8 54.9 1.0 CA A:ASN156 3.8 41.7 1.0 NE2 A:HIS241 4.2 41.3 1.0 N A:ASP160 4.2 47.0 1.0 O A:HOH520 4.2 49.5 1.0 N A:ASN156 4.2 38.9 1.0 CA A:LYS159 4.2 44.2 1.0 C A:LYS159 4.3 44.5 1.0 O A:HOH506 4.3 53.8 1.0 OD2 A:ASP160 4.3 66.2 1.0 N A:LYS159 4.3 42.4 1.0 N A:VAL158 4.4 41.8 1.0 N A:TYR157 4.5 39.0 1.0 CA A:VAL158 4.7 42.2 1.0 C A:TYR157 4.8 43.4 1.0 CA A:ASP160 4.8 49.1 1.0 CB A:ASP160 4.8 55.2 1.0 CE1 A:HIS241 4.8 39.9 1.0 O A:HOH523 4.9 56.2 1.0 OD1 A:ASN156 4.9 49.3 1.0 O A:LYS159 4.9 39.5 1.0

Potassium binding site 2 out of 2 in the GH24 Family Muramidase From Trichophaea Saccata with An SH3-Like Cell Wall Binding Domain


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of GH24 Family Muramidase From Trichophaea Saccata with An SH3-Like Cell Wall Binding Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:K301 b:60.5 occ:1.00 O B:HOH531 2.3 40.3 1.0 O B:HOH540 2.4 40.6 1.0 O B:SER155 2.7 39.1 1.0 O B:VAL158 2.7 39.5 1.0 OD1 B:ASP160 2.8 49.9 1.0 O B:ASN156 2.8 37.6 1.0 O B:HOH403 3.2 48.3 1.0 C B:ASN156 3.4 37.9 1.0 CG B:ASP160 3.5 52.1 1.0 C B:VAL158 3.6 40.9 1.0 C B:SER155 3.7 38.9 1.0 CA B:ASN156 3.7 37.1 1.0 O B:HOH436 3.8 49.4 1.0 OD2 B:ASP160 3.9 57.5 1.0 N B:ASP160 3.9 40.6 1.0 O B:HOH483 4.1 52.4 1.0 C B:LYS159 4.1 38.6 1.0 CA B:LYS159 4.2 42.4 1.0 N B:ASN156 4.2 36.6 1.0 O B:HOH516 4.2 49.9 1.0 NE2 B:HIS241 4.3 44.4 1.0 N B:LYS159 4.3 39.7 1.0 N B:TYR157 4.4 36.5 1.0 N B:VAL158 4.4 36.5 1.0 O B:HOH548 4.4 54.0 1.0 CA B:ASP160 4.5 45.3 1.0 CB B:ASP160 4.5 49.1 1.0 CA B:VAL158 4.7 37.8 1.0 C B:TYR157 4.7 40.1 1.0 OD1 B:ASN156 4.8 59.1 1.0 O B:LYS159 4.8 37.7 1.0 CE1 B:HIS241 4.9 38.1 1.0 O B:HOH533 4.9 60.4 1.0 CA B:SER155 5.0 36.0 0.5 CA B:SER155 5.0 36.8 0.5

O.V.Moroz, E.Blagova, A.A.Lebedev, L.K.Skov, R.A.Pache, K.M.Schnorr, L.Kiemer, E.P.Friis, S.Nymand-Grarup, L.Ming, L.Ye, M.Klausen, M.T.Cohn, E.G.W.Schmidt, G.J.Davies, K.S.Wilson. Module Walking Using An SH3-Like Cell-Wall-Binding Domain Leads to A New GH184 Family of Muramidases. Acta Crystallogr D Struct 2023BIOL.
ISSN: ISSN 2059-7983
PubMed: 37428847
DOI: 10.1107/S2059798323005004