Potassium in PDB 8azf: Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine

All present enzymatic activity of Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine:
3.3.1.1;

The structure of Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine, PDB code: 8azf was solved by A.Arning, P.Malecki, K.Wozniak, K.Brzezinski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	67.13 / 1.41
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	142.881, 85.916, 111.824, 90, 122.08, 90
R / Rfree (%)	16.7 / 22.5

The binding sites of Potassium atom in the Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine (pdb code 8azf). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine, PDB code: 8azf:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine within 5.0Å range: probe atom residue distance (Å) B Occ A:K503 b:11.5 occ:1.00 O A:HIS382 2.7 10.9 1.0 O A:THR380 2.7 11.0 1.0 O A:HOH897 2.8 14.0 1.0 O A:HOH822 2.8 12.7 1.0 OG1 A:THR380 2.8 12.9 1.0 O C:HOH897 3.0 12.8 1.0 OE1 A:GLN65 3.2 11.2 1.0 NE2 A:GLN65 3.3 10.7 1.0 CD A:GLN65 3.5 12.2 1.0 CB A:THR380 3.5 12.1 1.0 C A:THR380 3.6 12.0 1.0 C A:HIS382 3.6 10.9 1.0 O A:GLY381 3.7 12.4 1.0 CA A:PRO383 4.0 10.8 1.0 N6 A:ADN502 4.0 11.5 1.0 C A:GLY381 4.0 12.3 1.0 N A:PRO383 4.1 10.6 1.0 CA A:THR380 4.2 10.6 1.0 CB C:ASP216 4.3 14.2 1.0 N A:HIS382 4.4 10.2 1.0 N A:GLY381 4.5 9.6 1.0 O A:HOH1028 4.5 15.4 1.0 CG C:ASP216 4.6 15.8 1.0 CA A:HIS382 4.6 10.2 1.0 CG A:GLN65 4.6 11.8 1.0 OD1 C:ASP216 4.7 13.2 1.0 C A:PRO383 4.7 11.7 1.0 CA A:GLY381 4.7 12.1 1.0 N A:SER384 4.8 10.6 1.0 CG2 A:THR380 4.8 11.8 1.0 OE1 A:GLN91 4.8 11.6 1.0 O C:ASP216 5.0 13.1 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine within 5.0Å range: probe atom residue distance (Å) B Occ C:K503 b:11.6 occ:1.00 O C:THR380 2.7 11.0 1.0 O C:HIS382 2.7 10.7 1.0 O C:HOH907 2.8 13.6 1.0 O C:HOH851 2.8 13.0 1.0 OG1 C:THR380 2.8 12.6 1.0 O A:HOH855 3.0 12.3 1.0 OE1 C:GLN65 3.2 12.2 1.0 NE2 C:GLN65 3.3 11.7 1.0 CD C:GLN65 3.5 12.1 1.0 CB C:THR380 3.6 12.8 1.0 C C:THR380 3.6 10.9 1.0 C C:HIS382 3.6 11.8 1.0 O C:GLY381 3.7 12.2 1.0 CA C:PRO383 4.0 11.0 1.0 N6 C:ADN502 4.0 10.8 1.0 C C:GLY381 4.0 11.1 1.0 N C:PRO383 4.1 10.9 1.0 CA C:THR380 4.2 11.4 1.0 CB A:ASP216 4.3 11.9 1.0 N C:HIS382 4.4 9.6 1.0 O C:HOH1030 4.5 16.3 1.0 N C:GLY381 4.5 9.8 1.0 CG A:ASP216 4.6 14.0 1.0 OD1 A:ASP216 4.6 15.0 1.0 CG C:GLN65 4.6 11.9 1.0 CA C:HIS382 4.7 9.9 1.0 C C:PRO383 4.7 11.8 1.0 CA C:GLY381 4.7 10.4 1.0 N C:SER384 4.7 9.5 1.0 OE1 C:GLN91 4.8 11.4 1.0 CG2 C:THR380 4.9 11.3 1.0 O A:ASP216 5.0 13.1 1.0

A.Arning, P.Malecki, K.Wozniak, K.Brzezinski. Crystal Structure of K449E Variant of S-Adenosyl-L-Homocysteine Hydrolase From Pseudomonas Aeruginosa in Complex with Adenosine To Be Published.