Potassium in PDB 7z4l: SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State

The structure of SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Potassium atom in the SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State (pdb code 7z4l). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State, PDB code: 7z4l:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State within 5.0Å range: probe atom residue distance (Å) B Occ A:K202 b:114.3 occ:1.00 OG1 B:THR1102 3.1 73.5 1.0 N4 A:C67 3.2 60.3 1.0 O4 A:U66 3.3 61.0 1.0 O B:VAL1100 3.4 66.0 1.0 N B:THR1102 4.0 66.6 1.0 CB B:THR1102 4.2 71.6 1.0 CG2 B:THR1102 4.2 73.5 1.0 OE1 B:GLU1099 4.3 79.6 1.0 C4 A:U66 4.3 53.9 1.0 OP2 A:A65 4.4 47.8 1.0 C4 A:C67 4.4 55.5 1.0 C B:VAL1100 4.6 61.9 1.0 CA B:THR1102 4.7 69.8 1.0 CA B:GLN1101 4.7 60.8 1.0 C B:GLN1101 4.8 66.6 1.0 O B:THR1102 5.0 75.1 1.0 CB B:GLU1099 5.0 65.6 1.0

Potassium binding site 2 out of 2 in the SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of SPCAS9 Bound to 18-Nucleotide Complementary Dna Substrate in the Checkpoint State within 5.0Å range: probe atom residue distance (Å) B Occ A:K203 b:81.7 occ:1.00 O B:GLN1350 2.7 63.2 1.0 O4' A:A68 2.8 54.0 1.0 C1' A:A68 3.4 51.2 1.0 OG1 B:THR1098 3.7 63.6 1.0 C4' A:A68 3.9 48.6 1.0 C B:GLN1350 3.9 59.0 1.0 O5' A:A68 4.0 56.1 1.0 N9 A:A68 4.2 47.6 1.0 C8 A:A68 4.4 46.2 1.0 N B:THR1098 4.4 55.4 1.0 O B:SER1351 4.4 53.9 1.0 C5' A:A68 4.5 50.2 1.0 C B:SER1351 4.6 51.9 1.0 C2' A:A68 4.6 49.1 1.0 O2' A:A68 4.6 55.2 1.0 N B:GLN1350 4.6 57.9 1.0 CA B:GLN1350 4.7 51.4 1.0 CG2 B:THR1098 4.7 51.8 1.0 CB B:THR1098 4.7 47.6 1.0 CB B:GLN1350 4.8 53.9 1.0 P A:A68 4.8 65.7 1.0 N B:SER1351 4.8 51.4 1.0 CA B:SER1351 4.8 52.2 1.0 CG1 B:VAL1095 4.8 50.8 1.0 OP1 A:A68 4.9 53.1 1.0 OP2 A:A68 4.9 57.8 1.0 C3' A:A68 5.0 46.5 1.0

M.Pacesa, L.Loeff, I.Querques, L.M.Muckenfuss, M.Sawicka, M.Jinek. R-Loop Formation and Conformational Activation Mechanisms of CAS9. Nature V. 609 191 2022.
ISSN: ESSN 1476-4687
PubMed: 36002571
DOI: 10.1038/S41586-022-05114-0