Potassium in PDB 7z4i: SPCAS9 Bound to 16-Nucleotide Complementary Dna Substrate

The binding sites of Potassium atom in the SPCAS9 Bound to 16-Nucleotide Complementary Dna Substrate (pdb code 7z4i). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the SPCAS9 Bound to 16-Nucleotide Complementary Dna Substrate, PDB code: 7z4i:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the SPCAS9 Bound to 16-Nucleotide Complementary Dna Substrate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of SPCAS9 Bound to 16-Nucleotide Complementary Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:K201 b:137.6 occ:1.00 OP2 A:A65 2.7 108.2 1.0 O4 A:U66 2.8 110.7 1.0 OG1 B:THR1102 2.8 140.5 1.0 C4 A:U66 3.6 110.3 1.0 C5 A:U66 3.8 108.6 1.0 P A:A65 4.0 113.6 1.0 CB B:THR1102 4.2 144.2 1.0 OP1 A:U64 4.3 111.6 1.0 O5' A:A65 4.4 107.2 1.0 N7 A:A65 4.4 105.0 1.0 OP2 A:U64 4.5 110.9 1.0 N4 A:C67 4.6 114.4 1.0 CG2 B:THR1102 4.7 144.3 1.0 C8 A:A65 4.8 104.6 1.0 P A:U64 4.8 116.2 1.0 OP1 A:A65 4.8 106.5 1.0 N3 A:U66 4.9 113.0 1.0

Potassium binding site 2 out of 2 in the SPCAS9 Bound to 16-Nucleotide Complementary Dna Substrate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of SPCAS9 Bound to 16-Nucleotide Complementary Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:K202 b:161.4 occ:1.00 O B:GLN1350 2.7 146.5 1.0 O4' A:A68 3.3 94.4 1.0 C B:GLN1350 3.9 144.2 1.0 C1' A:A68 3.9 99.9 1.0 O B:SER1351 4.1 121.4 1.0 OG1 B:THR1098 4.1 126.8 1.0 C B:SER1351 4.2 122.9 1.0 O5' A:A68 4.3 106.6 1.0 C4' A:A68 4.4 96.9 1.0 CG1 B:VAL1095 4.4 125.9 1.0 N B:THR1098 4.4 119.9 1.0 N B:ILE1352 4.5 117.1 1.0 CA B:SER1351 4.6 120.3 1.0 N9 A:A68 4.6 98.9 1.0 CA B:ILE1352 4.7 116.9 1.0 N B:SER1351 4.7 124.0 1.0 CA B:LYS1097 4.7 122.8 1.0 C8 A:A68 4.8 104.7 1.0 CA B:GLN1350 4.8 140.8 1.0 CG2 B:THR1098 4.9 123.2 1.0 O B:LYS1096 4.9 118.8 1.0 CB B:GLN1350 4.9 138.0 1.0 C5' A:A68 4.9 100.0 1.0 OP2 A:A68 4.9 112.0 1.0 P A:A68 5.0 123.1 1.0 N B:GLN1350 5.0 138.1 1.0 OP1 A:A68 5.0 108.4 1.0

M.Pacesa, L.Loeff, I.Querques, L.M.Muckenfuss, M.Sawicka, M.Jinek. R-Loop Formation and Conformational Activation Mechanisms of CAS9. Nature V. 609 191 2022.
ISSN: ESSN 1476-4687
PubMed: 36002571
DOI: 10.1038/S41586-022-05114-0