Potassium in PDB 7y46: Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp

The binding sites of Potassium atom in the Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp (pdb code 7y46). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 6 binding sites of Potassium where determined in the Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp, PDB code: 7y46:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6;

Potassium binding site 1 out of 6 in the Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp within 5.0Å range: probe atom residue distance (Å) B Occ C:K2102 b:413.6 occ:1.00 O C:ALA728 2.6 408.9 1.0 OD2 C:ASP747 2.8 434.0 1.0 O C:LYS726 2.9 358.9 1.0 O C:LEU725 3.0 368.6 1.0 C C:LYS726 3.5 358.9 1.0 C C:ALA728 3.8 408.9 1.0 CA C:LYS726 3.9 358.9 1.0 CG C:ASP747 3.9 434.0 1.0 C C:LEU725 4.1 368.6 1.0 N C:ALA728 4.2 408.9 1.0 O C:ASP729 4.2 427.9 1.0 OD1 C:ASP747 4.3 434.0 1.0 C C:ASP729 4.4 427.9 1.0 N C:LYS726 4.5 358.9 1.0 N C:LYS727 4.5 397.9 1.0 CA C:ALA728 4.6 408.9 1.0 C C:LYS727 4.7 397.9 1.0 N C:ILE730 4.8 412.5 1.0 N C:ASP729 4.8 427.9 1.0 N C:GLY731 4.9 379.6 1.0 CA C:ASP729 4.9 427.9 1.0 CA C:LYS727 4.9 397.9 1.0 CA C:ILE730 5.0 412.5 1.0

Potassium binding site 2 out of 6 in the Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp within 5.0Å range: probe atom residue distance (Å) B Occ C:K2103 b:360.2 occ:1.00 OG C:SER782 2.7 365.8 1.0 OD2 C:ASP811 2.8 348.5 1.0 OD1 C:ASN783 2.8 304.5 1.0 O C:THR779 2.8 277.5 1.0 O C:HOH2202 2.8 353.6 1.0 CG C:ASP811 3.3 348.5 1.0 OD1 C:ASP811 3.4 348.5 1.0 CG C:ASN783 3.8 304.5 1.0 CB C:SER782 3.9 365.8 1.0 C C:THR779 3.9 277.5 1.0 N C:ASN783 4.0 304.5 1.0 C C:SER782 4.1 365.8 1.0 OE1 C:GLU786 4.2 348.9 1.0 CG2 C:THR779 4.2 277.5 1.0 OE2 C:GLU786 4.2 348.9 1.0 CA C:SER782 4.4 365.8 1.0 CA C:ASN783 4.4 304.5 1.0 O C:SER782 4.4 365.8 1.0 OD2 C:ASP815 4.5 370.6 1.0 CD C:GLU786 4.5 348.9 1.0 CB C:ASP811 4.5 348.5 1.0 CA C:THR779 4.6 277.5 1.0 N C:SER782 4.6 365.8 1.0 ND2 C:ASN783 4.6 304.5 1.0 CB C:ASN783 4.7 304.5 1.0 K C:K2104 4.8 421.2 1.0 O C:LEU780 4.9 283.1 1.0 N C:LEU780 5.0 283.1 1.0 CA C:ASP811 5.0 348.5 1.0

Potassium binding site 3 out of 6 in the Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp within 5.0Å range: probe atom residue distance (Å) B Occ C:K2104 b:421.2 occ:1.00 O C:ALA330 2.8 376.6 1.0 OD2 C:ASP811 3.0 348.5 1.0 O C:VAL332 3.1 371.0 1.0 O C:VAL329 3.2 401.1 1.0 OE1 C:GLU786 3.2 348.9 1.0 CG C:ASP811 3.5 348.5 1.0 C C:ALA330 3.5 376.6 1.0 OE1 C:GLU334 3.7 394.7 1.0 OD1 C:ASP811 3.8 348.5 1.0 OD1 C:ASN783 3.8 304.5 1.0 C C:VAL332 3.9 371.0 1.0 CA C:ALA330 4.0 376.6 1.0 CG2 C:ILE807 4.0 382.6 1.0 C C:VAL329 4.2 401.1 1.0 CD C:GLU786 4.3 348.9 1.0 CA C:PRO333 4.4 331.2 1.0 N C:PRO333 4.5 331.2 1.0 N C:VAL332 4.5 371.0 1.0 C C:ASN331 4.5 382.6 1.0 CB C:ASP811 4.5 348.5 1.0 N C:ASN331 4.5 382.6 1.0 CD C:GLU334 4.6 394.7 1.0 CG C:ASN783 4.6 304.5 1.0 N C:ALA330 4.6 376.6 1.0 OE2 C:GLU334 4.6 394.7 1.0 O C:ASN331 4.7 382.6 1.0 CA C:VAL332 4.8 371.0 1.0 K C:K2103 4.8 360.2 1.0 OE2 C:GLU786 4.8 348.9 1.0 CA C:ASN331 4.9 382.6 1.0

Potassium binding site 4 out of 6 in the Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp within 5.0Å range: probe atom residue distance (Å) B Occ A:K2102 b:404.6 occ:1.00 O A:ALA728 2.6 417.2 1.0 OD2 A:ASP747 2.8 436.8 1.0 O A:LYS726 2.9 371.9 1.0 O A:LEU725 3.0 379.3 1.0 C A:LYS726 3.5 371.9 1.0 C A:ALA728 3.8 417.2 1.0 CA A:LYS726 3.9 371.9 1.0 CG A:ASP747 3.9 436.8 1.0 C A:LEU725 4.1 379.3 1.0 N A:ALA728 4.2 417.2 1.0 O A:ASP729 4.2 427.9 1.0 OD1 A:ASP747 4.3 436.8 1.0 C A:ASP729 4.4 427.9 1.0 N A:LYS726 4.5 371.9 1.0 N A:LYS727 4.5 405.8 1.0 CA A:ALA728 4.6 417.2 1.0 C A:LYS727 4.7 405.8 1.0 N A:ILE730 4.8 411.5 1.0 N A:ASP729 4.8 427.9 1.0 N A:GLY731 4.9 381.3 1.0 CA A:ASP729 4.9 427.9 1.0 CA A:LYS727 4.9 405.8 1.0 CA A:ILE730 5.0 411.5 1.0

Potassium binding site 5 out of 6 in the Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp within 5.0Å range: probe atom residue distance (Å) B Occ A:K2103 b:366.7 occ:1.00 OG A:SER782 2.7 366.2 1.0 OD2 A:ASP811 2.8 357.2 1.0 OD1 A:ASN783 2.8 298.2 1.0 O A:THR779 2.8 278.4 1.0 O A:HOH2202 2.8 357.1 1.0 CG A:ASP811 3.3 357.2 1.0 OD1 A:ASP811 3.4 357.2 1.0 CG A:ASN783 3.8 298.2 1.0 CB A:SER782 3.9 366.2 1.0 C A:THR779 3.9 278.4 1.0 N A:ASN783 4.0 298.2 1.0 C A:SER782 4.1 366.2 1.0 OE1 A:GLU786 4.2 349.6 1.0 CG2 A:THR779 4.2 278.4 1.0 OE2 A:GLU786 4.2 349.6 1.0 CA A:SER782 4.4 366.2 1.0 CA A:ASN783 4.4 298.2 1.0 O A:SER782 4.4 366.2 1.0 OD2 A:ASP815 4.5 371.7 1.0 CD A:GLU786 4.5 349.6 1.0 CB A:ASP811 4.5 357.2 1.0 CA A:THR779 4.6 278.4 1.0 N A:SER782 4.6 366.2 1.0 ND2 A:ASN783 4.6 298.2 1.0 CB A:ASN783 4.7 298.2 1.0 K A:K2104 4.8 425.9 1.0 O A:LEU780 4.9 288.9 1.0 N A:LEU780 5.0 288.9 1.0 CA A:ASP811 5.0 357.2 1.0

Potassium binding site 6 out of 6 in the Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Cryo-Em Structure of the Na+,K+-Atpase in the E2.2K+ State After Addition of Atp within 5.0Å range: probe atom residue distance (Å) B Occ A:K2104 b:425.9 occ:1.00 O A:ALA330 2.8 377.9 1.0 OD2 A:ASP811 3.0 357.2 1.0 O A:VAL332 3.1 380.7 1.0 O A:VAL329 3.2 392.8 1.0 OE1 A:GLU786 3.2 349.6 1.0 CG A:ASP811 3.5 357.2 1.0 C A:ALA330 3.5 377.9 1.0 OE1 A:GLU334 3.7 391.2 1.0 OD1 A:ASP811 3.8 357.2 1.0 OD1 A:ASN783 3.8 298.2 1.0 C A:VAL332 3.9 380.7 1.0 CA A:ALA330 4.0 377.9 1.0 CG2 A:ILE807 4.0 383.5 1.0 C A:VAL329 4.2 392.8 1.0 CD A:GLU786 4.3 349.6 1.0 CA A:PRO333 4.4 341.1 1.0 N A:PRO333 4.5 341.1 1.0 N A:VAL332 4.5 380.7 1.0 C A:ASN331 4.5 394.9 1.0 CB A:ASP811 4.5 357.2 1.0 N A:ASN331 4.5 394.9 1.0 CD A:GLU334 4.6 391.2 1.0 CG A:ASN783 4.6 298.2 1.0 N A:ALA330 4.6 377.9 1.0 OE2 A:GLU334 4.6 391.2 1.0 O A:ASN331 4.7 394.9 1.0 CA A:VAL332 4.8 380.7 1.0 K A:K2103 4.8 366.7 1.0 OE2 A:GLU786 4.8 349.6 1.0 CA A:ASN331 4.9 394.9 1.0

R.Kanai, F.Cornelius, B.Vilsen, C.Toyoshima. Cryo-Electron Microscopy of Na + ,K + -Atpase Reveals How the Extracellular Gate Locks in the E2·2K + State. Febs Lett. V. 596 2513 2022.
ISSN: ISSN 0014-5793
PubMed: 35747985
DOI: 10.1002/1873-3468.14437