Potassium in PDB 7x22: Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State

All present enzymatic activity of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State:
7.2.2.13; 7.2.2.19;

The structure of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Fluorine	(F)	4 atoms
Aluminium	(Al)	1 atom

The binding sites of Potassium atom in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State (pdb code 7x22). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State, PDB code: 7x22:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State within 5.0Å range: probe atom residue distance (Å) B Occ A:K1103 b:84.8 occ:1.00 OD2 A:ASP823 2.6 82.1 1.0 O A:ALA342 2.6 76.5 1.0 OD1 A:ASN795 2.7 75.1 1.0 O A:VAL341 2.7 74.4 1.0 O A:VAL344 2.9 76.4 1.0 OE1 A:GLU798 3.2 75.5 1.0 C A:ALA342 3.2 76.5 1.0 CG A:ASP823 3.4 82.1 1.0 OD1 A:ASP823 3.5 82.1 1.0 CA A:ALA342 3.6 76.5 1.0 C A:VAL344 3.6 76.4 1.0 CG A:ASN795 3.7 75.1 1.0 C A:VAL341 3.8 74.4 1.0 N A:VAL344 3.9 76.4 1.0 OE1 A:GLU346 3.9 80.8 1.0 OE2 A:GLU346 4.1 80.8 1.0 N A:ASN343 4.1 78.3 1.0 CD A:GLU798 4.2 75.5 1.0 N A:ALA342 4.2 76.5 1.0 N A:PRO345 4.2 77.9 1.0 CA A:PRO345 4.4 77.9 1.0 ND2 A:ASN795 4.4 75.1 1.0 CA A:VAL344 4.4 76.4 1.0 CD A:GLU346 4.4 80.8 1.0 C A:ASN343 4.4 78.3 1.0 OE2 A:GLU798 4.4 75.5 1.0 K A:K1104 4.5 85.1 1.0 CA A:ASN795 4.6 75.1 1.0 CB A:ASN795 4.6 75.1 1.0 CA A:ASN343 4.7 78.3 1.0 CB A:ASP823 4.7 82.1 1.0 CB A:ALA342 4.9 76.5 1.0

Potassium binding site 2 out of 3 in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State within 5.0Å range: probe atom residue distance (Å) B Occ A:K1104 b:85.1 occ:1.00 OD1 A:ASN795 2.6 75.1 1.0 OG A:SER794 2.7 72.5 1.0 O A:HOH1201 2.7 81.7 1.0 OD1 A:ASP823 2.8 82.1 1.0 O A:THR791 2.8 69.7 1.0 CG A:ASN795 3.2 75.1 1.0 N A:ASN795 3.5 75.1 1.0 C A:THR791 3.7 69.7 1.0 ND2 A:ASN795 3.9 75.1 1.0 CG A:ASP823 3.9 82.1 1.0 CB A:SER794 4.0 72.5 1.0 CA A:ASN795 4.0 75.1 1.0 CB A:ASN795 4.0 75.1 1.0 CA A:THR791 4.0 69.7 1.0 OE2 A:GLU798 4.1 75.5 1.0 C A:SER794 4.1 72.5 1.0 OD1 A:ASP827 4.2 78.5 1.0 CB A:THR791 4.2 69.7 1.0 CB A:PRO345 4.3 77.9 1.0 K A:K1103 4.5 84.8 1.0 CA A:PRO345 4.5 77.9 1.0 CA A:SER794 4.5 72.5 1.0 OD2 A:ASP823 4.6 82.1 1.0 O A:SER794 4.8 72.5 1.0 N A:LEU792 4.8 69.2 1.0 CD A:GLU798 4.9 75.5 1.0 N A:SER794 4.9 72.5 1.0 CB A:ASP823 4.9 82.1 1.0

Potassium binding site 3 out of 3 in the Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Cryo-Em Structure of Non Gastric H,K-Atpase ALPHA2 K794S in (2K+)E2- Alf State within 5.0Å range: probe atom residue distance (Å) B Occ A:K1105 b:87.2 occ:1.00 OD2 A:ASP759 2.8 81.4 1.0 O A:ALA740 3.0 77.1 1.0 O A:LYS738 3.0 82.3 1.0 O A:LEU737 3.0 78.0 1.0 CG A:ASP759 3.7 81.4 1.0 C A:LYS738 3.7 82.3 1.0 OD1 A:ASP759 3.8 81.4 1.0 CA A:LYS738 3.9 82.3 1.0 C A:LEU737 4.1 78.0 1.0 O A:ASP741 4.1 76.9 1.0 C A:ALA740 4.2 77.1 1.0 N A:LYS738 4.5 82.3 1.0 N A:GLY743 4.5 74.8 1.0 C A:ASP741 4.5 76.9 1.0 N A:ALA740 4.8 77.1 1.0 O A:ALA757 4.8 85.5 1.0 N A:LYS739 4.8 80.4 1.0 CA A:ILE742 5.0 74.9 1.0 N A:ILE742 5.0 74.9 1.0 C A:LYS739 5.0 80.4 1.0 C A:ILE742 5.0 74.9 1.0

V.C.Young, H.Nakanishi, D.J.Meyer, T.Nishizawa, A.Oshima, P.Artigas, K.Abe. Structure and Function of H + /K + Pump Mutants Reveal Na + /K + Pump Mechanisms. Nat Commun V. 13 5270 2022.
ISSN: ESSN 2041-1723
PubMed: 36085139
DOI: 10.1038/S41467-022-32793-0