Potassium in PDB 7x20: Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State

All present enzymatic activity of Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State:
7.2.2.13; 7.2.2.19;

The structure of Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State, PDB code: 7x20 was solved by H.Nakanishi, K.Abe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.06 / 3.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	75.97, 109.06, 267.67, 90, 90, 90
R / Rfree (%)	26.5 / 31.6

The structure of Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State also contains other interesting chemical elements:

Fluorine	(F)	4 atoms
Aluminium	(Al)	1 atom

The binding sites of Potassium atom in the Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State (pdb code 7x20). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State, PDB code: 7x20:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State within 5.0Å range: probe atom residue distance (Å) B Occ A:K1102 b:23.1 occ:1.00 OD2 A:ASP823 2.7 23.1 1.0 O A:VAL344 2.7 21.4 1.0 OE1 A:GLU346 2.7 23.0 1.0 O A:VAL341 2.8 23.4 1.0 O A:ALA342 2.8 20.1 1.0 OE1 A:GLU798 3.0 17.6 1.0 OD1 A:ASN795 3.1 17.0 1.0 CG A:ASP823 3.4 20.1 1.0 C A:ALA342 3.5 21.0 1.0 CD A:GLU346 3.5 23.8 1.0 OE2 A:GLU346 3.6 24.0 1.0 C A:VAL344 3.6 20.9 1.0 OD1 A:ASP823 3.7 17.2 1.0 CG A:ASN795 3.9 18.9 1.0 C A:VAL341 3.9 24.0 1.0 CA A:ALA342 4.0 21.9 1.0 CD A:GLU798 4.0 17.7 1.0 N A:VAL344 4.2 21.4 1.0 OE2 A:GLU798 4.3 19.3 1.0 C A:ASN343 4.3 20.3 1.0 N A:PRO345 4.4 20.0 1.0 CA A:PRO345 4.4 19.7 1.0 N A:GLU346 4.4 21.4 1.0 N A:ASN343 4.4 21.2 1.0 N A:ALA342 4.4 23.4 1.0 CA A:VAL344 4.5 21.5 1.0 ND2 A:ASN795 4.5 19.4 1.0 CB A:ASP823 4.5 17.9 1.0 CG2 A:ILE819 4.5 18.4 1.0 O A:ASN343 4.6 19.4 1.0 CB A:ASN795 4.7 15.1 1.0 CA A:ASN343 4.8 20.4 1.0 C A:PRO345 4.9 20.6 1.0 CG A:GLU346 5.0 24.5 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Non Gastric H,K-Atpase ALPHA2 in (K+)E2-Alf State within 5.0Å range: probe atom residue distance (Å) B Occ A:K1103 b:24.7 occ:1.00 OD1 A:ASP759 2.6 20.9 1.0 O A:LYS738 2.9 24.5 1.0 OG A:SER291 3.0 33.6 1.0 O A:LEU737 3.2 18.8 1.0 O A:ALA740 3.3 25.5 1.0 CG A:ASP759 3.6 20.9 1.0 C A:LYS738 3.7 23.2 1.0 OD2 A:ASP759 3.9 21.9 1.0 CA A:LYS738 3.9 23.8 1.0 N A:GLY743 4.1 18.8 1.0 CB A:SER291 4.2 38.4 1.0 O A:ALA757 4.2 24.8 1.0 C A:LEU737 4.3 20.8 1.0 C A:ALA740 4.3 20.5 1.0 CA A:ILE742 4.5 19.7 1.0 O A:ASP741 4.5 19.2 1.0 C A:ILE742 4.5 19.7 1.0 N A:LYS738 4.6 22.9 1.0 N A:ALA740 4.7 19.2 1.0 C A:ASP741 4.7 18.9 1.0 NZ A:LYS738 4.8 38.6 1.0 N A:ILE742 4.8 18.0 1.0 CA A:GLY743 4.8 17.5 1.0 CD A:LYS738 4.9 24.4 1.0 CB A:ASP759 4.9 20.6 1.0 N A:LYS739 4.9 21.4 1.0 CA A:ALA740 4.9 18.3 1.0 CE A:LYS738 4.9 33.9 1.0

V.C.Young, H.Nakanishi, D.J.Meyer, T.Nishizawa, A.Oshima, P.Artigas, K.Abe. Structure and Function of H + /K + Pump Mutants Reveal Na + /K + Pump Mechanisms. Nat Commun V. 13 5270 2022.
ISSN: ESSN 2041-1723
PubMed: 36085139
DOI: 10.1038/S41467-022-32793-0