Potassium in PDB 7sqw: Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation

The structure of Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation, PDB code: 7sqw was solved by L.G.Cuello, A.J.Labro, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.69 / 3.21
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	153.962, 153.962, 75.804, 90, 90, 90
R / Rfree (%)	23.2 / 27.3

The binding sites of Potassium atom in the Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation (pdb code 7sqw). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 5 binding sites of Potassium where determined in the Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation, PDB code: 7sqw:
Jump to Potassium binding site number: 1; 2; 3; 4; 5;

Potassium binding site 1 out of 5 in the Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation within 5.0Å range: probe atom residue distance (Å) B Occ C:K202 b:61.8 occ:0.25 O C:TYR78 4.1 50.0 1.0 O C:GLY79 4.5 65.6 1.0 C C:GLY79 4.8 57.5 1.0 CA C:GLY79 4.8 54.6 1.0 K C:K203 4.9 48.8 0.2

Potassium binding site 2 out of 5 in the Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation within 5.0Å range: probe atom residue distance (Å) B Occ C:K203 b:48.8 occ:0.25 O C:TYR78 2.7 50.0 1.0 O C:GLY77 2.9 51.5 1.0 C C:TYR78 3.4 45.1 1.0 C C:GLY77 3.8 47.9 1.0 CA C:TYR78 3.9 45.1 1.0 N C:GLY79 4.3 44.4 1.0 N C:TYR78 4.3 51.6 1.0 CA C:GLY79 4.6 54.6 1.0 K C:K202 4.9 61.8 0.2

Potassium binding site 3 out of 5 in the Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation within 5.0Å range: probe atom residue distance (Å) B Occ C:K204 b:55.2 occ:0.25 O C:VAL76 2.7 52.3 1.0 O C:THR75 2.8 56.1 1.0 C C:VAL76 3.5 51.2 1.0 K C:K205 3.8 38.6 0.2 C C:THR75 3.9 51.9 1.0 CA C:VAL76 3.9 46.6 1.0 N C:VAL76 4.4 48.9 1.0 N C:GLY77 4.6 54.0 1.0 O C:GLY77 4.9 51.5 1.0

Potassium binding site 4 out of 5 in the Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation within 5.0Å range: probe atom residue distance (Å) B Occ C:K205 b:38.6 occ:0.25 OG1 C:THR75 2.8 38.0 1.0 O C:THR75 3.2 56.1 1.0 CB C:THR75 3.6 43.0 1.0 K C:K204 3.8 55.2 0.2 C C:THR75 4.2 51.9 1.0 CA C:THR75 4.6 50.1 1.0 CG2 C:THR75 4.7 52.0 1.0

Potassium binding site 5 out of 5 in the Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Structure of the Kcsa-W67F Mutant with the Activation Gate in the Closed Conformation within 5.0Å range:

L.Coonen, E.Martinez-Morales, D.V.Van De Sande, D.J.Snyders, D.M.Cortes, L.G.Cuello, A.J.Labro. The Nonconducting W434F Mutant Adopts Upon Membrane Depolarization An Inactivated-Like State That Differs From Wild-Type Shaker-Ir Potassium Channels. Sci Adv V. 8 N1731 2022.
ISSN: ESSN 2375-2548
PubMed: 36112676
DOI: 10.1126/SCIADV.ABN1731