Potassium in PDB 7s2x: Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis

The structure of Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis, PDB code: 7s2x was solved by P.Y.-T.Chen, D.B.B.Trivella, K.D.Bauman, B.S.Moore, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.85 / 2.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	84.33, 218.29, 87.87, 90, 111.01, 90
R / Rfree (%)	19.7 / 23

The binding sites of Potassium atom in the Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis (pdb code 7s2x). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis, PDB code: 7s2x:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis within 5.0Å range: probe atom residue distance (Å) B Occ A:K601 b:62.3 occ:1.00 O B:ILE274 2.9 38.2 1.0 OE1 A:GLU172 2.9 44.2 1.0 O A:GLN198 3.6 49.1 1.0 C B:ILE274 4.1 37.8 1.0 CD A:GLU172 4.1 43.1 1.0 CB A:GLN198 4.4 48.5 1.0 C A:GLN198 4.5 49.5 1.0 N B:ILE274 4.6 36.7 1.0 CB B:ASN275 4.6 41.2 1.0 CA A:GLN198 4.7 48.7 1.0 OE2 A:GLU172 4.8 42.8 1.0 CA B:ILE274 5.0 36.2 1.0 N B:ASN275 5.0 38.5 1.0

Potassium binding site 2 out of 4 in the Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis within 5.0Å range: probe atom residue distance (Å) B Occ B:K601 b:52.4 occ:1.00 O B:PHE532 2.8 52.9 1.0 O B:ALA530 2.9 57.5 1.0 C B:PHE532 3.8 52.8 1.0 C B:ALA530 3.9 56.6 1.0 CA B:ALA530 4.4 57.4 1.0 N B:PHE532 4.5 53.1 1.0 N B:ARG533 4.6 52.7 1.0 CA B:ARG533 4.6 51.5 1.0 C B:ARG531 4.6 54.4 1.0 CA B:PHE532 4.8 53.0 1.0 O B:ARG531 4.8 55.0 1.0 N B:ARG531 4.9 55.5 1.0 O B:LEU529 4.9 53.7 1.0

Potassium binding site 3 out of 4 in the Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis within 5.0Å range: probe atom residue distance (Å) B Occ B:K602 b:48.4 occ:1.00 OH D:TYR73 3.1 39.2 1.0 OD1 B:ASP596 3.1 52.5 1.0 N D:GLN213 3.8 34.6 1.0 CZ D:TYR73 3.8 38.3 1.0 CE2 D:TYR73 3.8 37.3 1.0 CG B:ASP596 4.1 51.0 1.0 CZ2 D:TRP593 4.1 40.4 1.0 CB D:GLN213 4.2 34.9 1.0 OD2 B:ASP596 4.2 50.5 1.0 CH2 D:TRP593 4.3 39.6 1.0 CD2 D:LEU211 4.3 33.7 1.0 CA D:PRO212 4.4 32.8 1.0 CA D:GLN213 4.6 34.2 1.0 C D:PRO212 4.6 32.7 1.0 CB D:PRO212 4.8 33.4 1.0 CE2 D:TRP593 4.8 40.1 1.0 CE1 D:TYR73 5.0 37.2 1.0

Potassium binding site 4 out of 4 in the Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Structure of Salc, A Gamma-Lactam-Beta-Lactone Bicyclase For Salinosporamide Biosynthesis within 5.0Å range: probe atom residue distance (Å) B Occ C:K601 b:64.1 occ:1.00 O D:ILE274 2.9 45.0 1.0 OE1 C:GLU172 3.2 49.7 1.0 O C:GLN198 4.0 59.0 1.0 C D:ILE274 4.1 43.5 1.0 CB C:GLN198 4.3 57.5 1.0 CD C:GLU172 4.4 49.6 1.0 N D:ILE274 4.4 44.3 1.0 CB D:ASN275 4.6 46.0 1.0 CA C:GLN198 4.7 56.9 1.0 C C:GLN198 4.7 58.3 1.0 CG C:GLN198 4.8 56.3 1.0 OE1 C:GLN198 4.9 57.1 1.0 CA D:ILE274 4.9 43.6 1.0 N D:ASN275 5.0 44.8 1.0

K.D.Bauman, V.V.Shende, P.Y.Chen, D.B.B.Trivella, T.A.M.Gulder, S.Vellalath, D.Romo, B.S.Moore. Enzymatic Assembly of the Salinosporamide Gamma-Lactam-Beta-Lactone Anticancer Warhead. Nat.Chem.Biol. V. 18 538 2022.
ISSN: ESSN 1552-4469
PubMed: 35314816
DOI: 10.1038/S41589-022-00993-W