Potassium in PDB 7nf3: Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn

All present enzymatic activity of Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn:
4.1.1.102;

The structure of Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn, PDB code: 7nf3 was solved by A.Saaret, D.Leys, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.09 / 1.10
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	95.91, 63.85, 87.82, 90, 90, 90
R / Rfree (%)	15.8 / 17.2

The structure of Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn also contains other interesting chemical elements:

Manganese

(Mn)

1 atom

The binding sites of Potassium atom in the Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn (pdb code 7nf3). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn, PDB code: 7nf3:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn within 5.0Å range: probe atom residue distance (Å) B Occ A:K601 b:7.5 occ:1.00 OE2 A:GLU233 2.8 7.2 1.0 O A:MET225 2.8 8.0 1.0 O A:ALA222 2.8 7.9 1.0 O2P A:4LU603 2.9 6.5 1.0 O5' A:4LU603 3.0 6.7 1.0 O A:TRP169 3.1 6.9 1.0 O A:SER223 3.1 8.3 1.0 O A:HOH726 3.3 7.3 1.0 P A:4LU603 3.4 6.4 1.0 C A:SER223 3.5 7.2 1.0 CD A:GLU233 3.6 7.0 1.0 O1P A:4LU603 3.7 8.0 1.0 MN A:MN604 3.7 6.8 0.8 CA A:SER223 3.7 7.1 1.0 CG A:GLU233 3.8 7.6 1.0 C A:ALA222 3.9 7.2 1.0 C A:MET225 3.9 7.2 1.0 N A:MET225 4.1 7.2 1.0 C5' A:4LU603 4.2 6.8 1.0 C A:TRP169 4.2 6.6 1.0 O4' A:4LU603 4.3 8.0 1.0 N A:SER223 4.3 6.7 1.0 C4' A:4LU603 4.3 6.2 1.0 CB A:SER170 4.3 7.2 1.0 N A:SER224 4.3 6.8 0.5 N A:SER224 4.4 7.0 0.5 CA A:SER170 4.4 6.6 1.0 CA A:MET225 4.6 8.0 1.0 OE1 A:GLU233 4.7 7.5 1.0 C A:SER224 4.8 7.7 0.5 C A:SER224 4.8 7.5 0.5 O3P A:4LU603 4.8 7.1 1.0 ND2 A:ASN168 4.8 7.0 1.0 N A:SER170 4.9 6.2 1.0 CG1 A:ILE227 4.9 9.6 1.0 N A:ILE227 4.9 7.8 1.0 N A:PRO226 4.9 7.1 1.0 CB A:SER223 5.0 7.2 1.0 CA A:SER224 5.0 6.8 0.5 CA A:SER224 5.0 7.2 0.5

Potassium binding site 2 out of 2 in the Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of A. Niger Fdc T395M Variant (Anfdci) in Complex with Prfmn within 5.0Å range: probe atom residue distance (Å) B Occ A:K602 b:8.2 occ:1.00 O A:LEU461 2.7 10.0 1.0 O A:ARG421 2.7 8.2 1.0 O A:ASP459 2.7 8.2 1.0 O A:HOH864 2.8 13.7 1.0 O A:HOH1069 2.8 11.7 1.0 OD2 A:ASP427 2.8 8.3 1.0 CG A:ASP427 3.4 9.1 1.0 O A:HOH967 3.5 15.1 1.0 C A:LEU461 3.7 8.0 1.0 C A:ASP459 3.8 7.5 1.0 C A:ARG421 3.8 7.4 1.0 CB A:ASP427 3.9 8.3 1.0 OD1 A:ASP427 4.1 10.1 1.0 N A:LEU461 4.1 8.2 1.0 CA A:CYS422 4.1 7.5 1.0 O A:HOH759 4.3 10.1 1.0 CA A:ALA460 4.3 7.5 1.0 C A:ALA460 4.4 7.4 1.0 N A:ALA460 4.4 7.1 1.0 N A:CYS422 4.4 7.2 1.0 CG A:ASP459 4.5 10.4 1.0 CB A:ASP459 4.5 8.7 1.0 CA A:LEU461 4.6 8.3 1.0 N A:MET462 4.6 9.0 1.0 CA A:MET462 4.7 8.8 1.0 OD1 A:ASP459 4.7 11.3 1.0 N A:ARG423 4.7 6.9 1.0 CA A:ASP459 4.8 7.8 1.0 OD2 A:ASP459 4.8 14.0 1.0 CA A:ARG421 4.9 7.1 1.0 CB A:CYS422 5.0 8.0 1.0

A.Saaret, B.Villiers, F.Stricher, M.Anissimova, M.Cadillon, R.Spiess, S.Hay, D.Leys. Directed Evolution of Prenylated Fmn-Dependent Fdc Supports Efficient in Vivo Isobutene Production Nat Commun 2021.
ISSN: ESSN 2041-1723