Potassium in PDB 7mdj: The Structure of Kcsa in Complex with A Synthetic Fab

The structure of The Structure of Kcsa in Complex with A Synthetic Fab, PDB code: 7mdj was solved by A.Rohaim, T.Slezak, L.Blackowicz, A.Kossiakoff, B.Roux, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.01 / 2.75
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	138.409, 138.409, 71.16, 90, 90, 90
R / Rfree (%)	20.1 / 26.5

The binding sites of Potassium atom in the The Structure of Kcsa in Complex with A Synthetic Fab (pdb code 7mdj). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the The Structure of Kcsa in Complex with A Synthetic Fab, PDB code: 7mdj:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the The Structure of Kcsa in Complex with A Synthetic Fab


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of The Structure of Kcsa in Complex with A Synthetic Fab within 5.0Å range: probe atom residue distance (Å) B Occ C:K201 b:109.2 occ:0.25 K C:K203 2.3 137.6 0.2 OG1 C:THR75 2.7 68.2 1.0 O C:THR75 3.0 85.8 1.0 CB C:THR75 3.8 77.1 1.0 C C:THR75 4.1 80.6 1.0 O C:HOH302 4.4 198.6 1.0 CA C:THR75 4.6 79.8 1.0 O C:HOH307 4.8 134.1 1.0 O C:THR74 4.8 84.2 1.0 CG2 C:THR75 4.8 84.3 1.0

Potassium binding site 2 out of 4 in the The Structure of Kcsa in Complex with A Synthetic Fab


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of The Structure of Kcsa in Complex with A Synthetic Fab within 5.0Å range: probe atom residue distance (Å) B Occ C:K202 b:143.9 occ:0.25 O C:HOH302 2.7 198.6 1.0 O C:VAL76 3.0 104.6 1.0 O C:GLY77 3.3 87.2 1.0 K C:K203 3.3 137.6 0.2 C C:VAL76 3.8 90.4 1.0 C C:GLY77 3.9 86.4 1.0 K C:K204 4.1 127.6 0.2 CA C:GLY77 4.1 88.8 1.0 N C:GLY77 4.3 93.2 1.0 O C:THR75 4.4 85.8 1.0 O C:HOH304 4.7 232.2 1.0 CA C:VAL76 4.9 81.3 1.0

Potassium binding site 3 out of 4 in the The Structure of Kcsa in Complex with A Synthetic Fab


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of The Structure of Kcsa in Complex with A Synthetic Fab within 5.0Å range: probe atom residue distance (Å) B Occ C:K203 b:137.6 occ:0.25 K C:K201 2.3 109.2 0.2 O C:THR75 2.5 85.8 1.0 O C:HOH302 2.7 198.6 1.0 K C:K202 3.3 143.9 0.2 C C:THR75 3.7 80.6 1.0 OG1 C:THR75 3.8 68.2 1.0 CB C:THR75 4.2 77.1 1.0 O C:VAL76 4.3 104.6 1.0 C C:VAL76 4.5 90.4 1.0 N C:VAL76 4.6 79.6 1.0 CA C:THR75 4.6 79.8 1.0 CA C:VAL76 4.6 81.3 1.0

Potassium binding site 4 out of 4 in the The Structure of Kcsa in Complex with A Synthetic Fab


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of The Structure of Kcsa in Complex with A Synthetic Fab within 5.0Å range: probe atom residue distance (Å) B Occ C:K204 b:127.6 occ:0.25 O C:GLY77 2.7 87.2 1.0 C C:GLY77 3.9 86.4 1.0 C C:TYR78 4.1 85.5 1.0 K C:K202 4.1 143.9 0.2 O C:TYR78 4.2 98.9 1.0 N C:GLY79 4.3 78.5 1.0 CA C:TYR78 4.4 87.8 1.0 CA C:GLY79 4.6 79.7 1.0 N C:TYR78 4.6 88.0 1.0

A.Rohaim, T.Slezak, Y.H.Koh, L.Blachowicz, A.A.Kossiakoff, B.Roux. Engineering of A Synthetic Antibody Fragment For Structural and Functional Studies of K+ Channels. J.Gen.Physiol. V. 154 2022.
ISSN: ESSN 1540-7748
PubMed: 35234830
DOI: 10.1085/JGP.202112965