Potassium in PDB 6vax: Crystal Structure of Human Sdha-ADHAF2 Assembly Intermediate

All present enzymatic activity of Crystal Structure of Human Sdha-ADHAF2 Assembly Intermediate:
1.3.5.1;

The structure of Crystal Structure of Human Sdha-ADHAF2 Assembly Intermediate, PDB code: 6vax was solved by P.Sharma, E.Maklashina, G.Cecchini, T.M.Iverson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.05 / 2.59
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	210.558, 68.782, 126.578, 90.00, 93.16, 90.00
R / Rfree (%)	19.9 / 24.9

The binding sites of Potassium atom in the Crystal Structure of Human Sdha-ADHAF2 Assembly Intermediate (pdb code 6vax). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Human Sdha-ADHAF2 Assembly Intermediate, PDB code: 6vax:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Human Sdha-ADHAF2 Assembly Intermediate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Human Sdha-ADHAF2 Assembly Intermediate within 5.0Å range: probe atom residue distance (Å) B Occ A:K705 b:77.7 occ:1.00 O A:ALA442 2.7 59.4 1.0 O A:GLU440 2.7 52.9 1.0 O A:ASN409 2.7 58.0 1.0 O A:GLY411 2.8 45.9 1.0 O A:HOH801 2.8 70.4 1.0 O A:MET410 2.9 56.3 1.0 C A:MET410 3.2 57.1 1.0 C A:GLU440 3.6 58.4 1.0 C A:GLY411 3.6 65.3 1.0 CA A:MET410 3.7 61.0 1.0 C A:ASN409 3.8 57.5 1.0 N A:GLY411 3.8 56.8 1.0 C A:ALA442 3.8 57.6 1.0 CA A:GLU440 3.9 56.4 1.0 O A:HOH807 4.1 69.2 1.0 CA A:GLY411 4.2 58.3 1.0 O A:GLY439 4.2 62.1 1.0 N A:MET410 4.2 60.6 1.0 O A:HOH806 4.3 65.6 1.0 OH A:TYR408 4.3 73.7 1.0 N A:ALA442 4.4 42.8 1.0 N A:GLY412 4.5 52.0 1.0 CG A:GLU440 4.5 56.3 1.0 CE2 A:TYR408 4.6 61.4 1.0 N A:CYS443 4.6 64.0 1.0 N A:ALA441 4.6 54.1 1.0 C A:ALA441 4.6 56.9 1.0 CA A:CYS443 4.7 66.1 1.0 CZ A:TYR408 4.7 68.5 1.0 CA A:ALA442 4.8 58.4 1.0 CB A:GLU440 4.8 58.5 1.0 CA A:GLY412 4.8 56.4 1.0 N A:GLU440 5.0 56.2 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Human Sdha-ADHAF2 Assembly Intermediate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Human Sdha-ADHAF2 Assembly Intermediate within 5.0Å range: probe atom residue distance (Å) B Occ C:K704 b:74.2 occ:1.00 O C:ALA442 2.7 55.9 1.0 O C:ASN409 2.7 70.4 1.0 O C:HOH806 2.7 69.7 1.0 O C:GLU440 2.8 57.9 1.0 O C:GLY411 2.8 57.4 1.0 O C:MET410 2.9 55.6 1.0 C C:MET410 3.3 63.9 1.0 C C:GLU440 3.5 63.5 1.0 C C:GLY411 3.8 64.4 1.0 C C:ASN409 3.8 72.6 1.0 CA C:MET410 3.8 67.1 1.0 C C:ALA442 3.8 62.6 1.0 CA C:GLU440 3.8 67.7 1.0 N C:GLY411 3.9 61.8 1.0 O C:GLY439 4.1 68.2 1.0 OH C:TYR408 4.1 76.1 1.0 O C:HOH814 4.2 63.6 1.0 O C:HOH807 4.2 63.7 1.0 CE2 C:TYR408 4.3 68.9 1.0 N C:MET410 4.3 67.3 1.0 N C:ALA442 4.3 47.3 1.0 CA C:GLY411 4.3 64.7 1.0 CG C:GLU440 4.5 69.0 1.0 CZ C:TYR408 4.5 71.4 1.0 N C:ALA441 4.5 54.4 1.0 C C:ALA441 4.7 55.9 1.0 N C:CYS443 4.7 62.5 1.0 CA C:ALA442 4.7 64.0 1.0 CB C:GLU440 4.8 68.3 1.0 N C:GLY412 4.8 56.7 1.0 CA C:CYS443 4.8 62.9 1.0 N C:GLU440 4.9 58.6 1.0 C C:GLY439 4.9 57.7 1.0 CA C:ALA441 5.0 53.7 1.0

P.Sharma, E.Maklashina, G.Cecchini, T.M.Iverson. The Roles of SDHAF2 and Dicarboxylate in Covalent Flavinylation of Sdha, the Human Complex II Flavoprotein. Proc.Natl.Acad.Sci.Usa V. 117 23548 2020.
ISSN: ESSN 1091-6490
PubMed: 32887801
DOI: 10.1073/PNAS.2007391117