Potassium in PDB 6v74: Crystal Structure of Human PKM2 in Complex with L-Asparagine

All present enzymatic activity of Crystal Structure of Human PKM2 in Complex with L-Asparagine:
2.7.1.40;

The structure of Crystal Structure of Human PKM2 in Complex with L-Asparagine, PDB code: 6v74 was solved by S.Nandi, M.Dey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	58.62 / 2.32
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	80.990, 154.580, 92.150, 90.00, 102.54, 90.00
R / Rfree (%)	23.3 / 27.5

The structure of Crystal Structure of Human PKM2 in Complex with L-Asparagine also contains other interesting chemical elements:

Magnesium	(Mg)	4 atoms
Chlorine	(Cl)	2 atoms

The binding sites of Potassium atom in the Crystal Structure of Human PKM2 in Complex with L-Asparagine (pdb code 6v74). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Crystal Structure of Human PKM2 in Complex with L-Asparagine, PDB code: 6v74:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Crystal Structure of Human PKM2 in Complex with L-Asparagine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Human PKM2 in Complex with L-Asparagine within 5.0Å range: probe atom residue distance (Å) B Occ A:K601 b:56.6 occ:1.00 O A:THR41 3.2 59.2 1.0 N A:ARG43 3.3 44.8 1.0 CA A:ALA42 3.8 50.8 1.0 CE1 A:HIS379 3.9 35.3 1.0 CB A:ARG43 4.0 39.6 1.0 C A:ALA42 4.1 46.6 1.0 CE2 A:PHE470 4.2 48.1 1.0 C A:THR41 4.2 59.4 1.0 CA A:ARG43 4.3 40.6 1.0 CZ A:PHE470 4.3 45.7 1.0 N A:ALA42 4.5 49.9 1.0 CE1 A:PHE502 4.7 52.0 1.0 ND1 A:HIS379 4.8 42.4 1.0 NE2 A:HIS379 4.8 41.7 1.0 O A:ARG43 4.8 44.2 1.0 CB A:ALA42 4.9 51.1 1.0

Potassium binding site 2 out of 4 in the Crystal Structure of Human PKM2 in Complex with L-Asparagine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Human PKM2 in Complex with L-Asparagine within 5.0Å range: probe atom residue distance (Å) B Occ A:K602 b:53.7 occ:1.00 OD1 A:ASN75 2.7 36.9 1.0 OD1 A:ASP113 2.7 37.8 1.0 O A:THR114 2.8 39.2 1.0 OG A:SER77 2.9 41.5 1.0 OG A:SER243 3.5 37.3 1.0 C A:THR114 3.6 39.3 1.0 CG A:ASP113 3.6 39.8 1.0 CG A:ASN75 3.7 42.9 1.0 NZ A:LYS270 3.9 27.4 1.0 O A:ASP113 4.0 36.4 1.0 CB A:SER77 4.0 45.0 1.0 CA A:LYS115 4.1 44.3 1.0 C A:ASP113 4.1 37.0 1.0 N A:LYS115 4.2 43.3 1.0 CB A:ASP113 4.2 37.1 1.0 N A:SER77 4.2 44.9 1.0 ND2 A:ASN75 4.3 35.0 1.0 N A:THR114 4.4 29.5 1.0 NH2 A:ARG73 4.4 30.5 1.0 O A:LYS115 4.5 48.2 1.0 OD2 A:ASP113 4.5 40.3 1.0 CA A:THR114 4.6 37.8 1.0 CA A:SER77 4.6 45.8 1.0 C A:LYS115 4.6 41.1 1.0 CB A:SER243 4.8 30.9 1.0 N A:PHE76 4.8 37.6 1.0 CA A:ASP113 4.8 36.7 1.0 CB A:ASN75 4.9 38.8 1.0 O2 A:OXL605 4.9 43.1 1.0 CA A:ASN75 5.0 38.9 1.0

Potassium binding site 3 out of 4 in the Crystal Structure of Human PKM2 in Complex with L-Asparagine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Human PKM2 in Complex with L-Asparagine within 5.0Å range: probe atom residue distance (Å) B Occ C:K601 b:69.9 occ:1.00 OD1 C:ASN75 2.8 53.3 1.0 OD1 C:ASP113 2.8 55.6 1.0 O C:THR114 2.9 51.1 1.0 OG C:SER77 3.0 50.0 1.0 OG C:SER243 3.4 50.8 1.0 CG C:ASP113 3.6 48.6 1.0 C C:THR114 3.9 52.6 1.0 CG C:ASN75 3.9 50.5 1.0 NZ C:LYS270 3.9 50.7 1.0 CB C:SER77 4.1 54.4 1.0 O C:ASP113 4.2 51.3 1.0 CA C:LYS115 4.2 50.6 1.0 NH2 C:ARG73 4.3 47.8 1.0 O C:LYS115 4.3 52.7 1.0 CB C:ASP113 4.3 46.1 1.0 OD2 C:ASP113 4.4 50.9 1.0 C C:ASP113 4.4 50.2 1.0 N C:LYS115 4.4 54.5 1.0 ND2 C:ASN75 4.4 57.3 1.0 N C:SER77 4.5 52.4 1.0 C C:LYS115 4.6 50.0 1.0 CB C:SER243 4.7 45.6 1.0 N C:THR114 4.7 48.2 1.0 O3 C:OXL614 4.8 56.9 1.0 K C:K602 4.8 73.0 1.0 CA C:SER77 4.8 52.7 1.0 CA C:THR114 4.9 48.0 1.0 CB C:ASN75 5.0 46.3 1.0 CA C:ASP113 5.0 48.9 1.0

Potassium binding site 4 out of 4 in the Crystal Structure of Human PKM2 in Complex with L-Asparagine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Crystal Structure of Human PKM2 in Complex with L-Asparagine within 5.0Å range: probe atom residue distance (Å) B Occ C:K602 b:73.0 occ:1.00 OD2 C:ASP296 2.6 53.5 1.0 O3 C:OXL614 2.7 56.9 1.0 OE1 C:GLU272 2.7 61.9 1.0 O4 C:OXL614 2.7 52.2 1.0 C1 C:OXL614 3.4 54.5 1.0 C2 C:OXL614 3.5 47.7 1.0 O C:HOH719 3.5 61.1 1.0 CG C:ASP296 3.7 56.7 1.0 CD C:GLU272 3.7 51.1 1.0 NZ C:LYS270 3.7 50.7 1.0 OE2 C:GLU272 4.1 54.3 1.0 CB C:ASP296 4.1 54.9 1.0 O1 C:OXL614 4.6 59.5 1.0 O2 C:OXL614 4.6 48.9 1.0 CE1 C:PHE244 4.6 54.4 1.0 CE C:LYS270 4.7 47.9 1.0 OD1 C:ASP296 4.8 62.5 1.0 K C:K601 4.8 69.9 1.0 CG C:GLU272 5.0 45.4 1.0

S.Nandi, M.Dey. Biochemical and Structural Investigation of Pyurvate Kinase Muscle Isoform 2 Regulation By Amino Acids To Be Published.