Potassium in PDB 6i3k: Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide

All present enzymatic activity of Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide:
1.3.3.5;

The structure of Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide, PDB code: 6i3k was solved by T.Koval, L.Svecova, T.Skalova, P.Kolenko, J.Duskova, L.H.Ostergaard, J.Dohnalek, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.93 / 1.60
Space group	F 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	136.906, 201.803, 217.869, 90.00, 90.00, 90.00
R / Rfree (%)	13.1 / 15.4

The structure of Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide also contains other interesting chemical elements:

Iron	(Fe)	4 atoms
Copper	(Cu)	8 atoms
Sodium	(Na)	2 atoms

The binding sites of Potassium atom in the Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide (pdb code 6i3k). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide, PDB code: 6i3k:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide within 5.0Å range: probe atom residue distance (Å) B Occ A:K617 b:31.8 occ:1.00 O B:HOH1215 2.8 51.8 1.0 OE1 A:GLU35 2.8 17.2 1.0 O A:HOH1182 2.9 14.6 1.0 O A:THR26 3.0 16.3 1.0 OD2 B:ASP323 3.2 17.1 0.5 OG1 A:THR28 3.3 14.2 1.0 CD A:GLU35 3.6 18.4 1.0 N A:THR28 3.7 10.6 1.0 OE2 A:GLU35 3.7 23.4 1.0 C A:THR26 3.8 13.9 1.0 CG B:ASP323 3.9 17.7 0.5 OG1 A:THR26 3.9 16.4 1.0 O B:HOH1285 4.0 50.1 1.0 OD1 B:ASP323 4.1 19.1 0.5 O A:HOH959 4.2 27.6 1.0 CA A:VAL27 4.2 10.8 1.0 C A:VAL27 4.2 10.6 1.0 O A:HOH859 4.3 40.6 1.0 N A:VAL27 4.3 11.0 1.0 CB A:THR28 4.4 13.1 1.0 CA A:THR28 4.5 12.0 1.0 O B:HOH1317 4.7 43.0 1.0 O B:HOH710 4.8 11.2 0.5 CB A:THR26 4.8 14.8 1.0 CA A:THR26 4.9 12.8 1.0 OD2 B:ASP323 4.9 22.4 0.5 CG A:GLU35 5.0 15.6 1.0

Potassium binding site 2 out of 2 in the Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Bilirubin Oxidase From Myrothecium Verrucaria, Mutant W396A in Complex with Ferricyanide within 5.0Å range: probe atom residue distance (Å) B Occ B:K617 b:28.7 occ:1.00 OD2 A:ASP323 2.6 18.7 0.5 O B:HOH1135 2.8 18.8 1.0 OE1 B:GLU35 2.8 20.2 1.0 O B:THR26 3.0 18.4 1.0 OG1 B:THR28 3.1 17.0 1.0 O A:HOH906 3.2 31.2 1.0 CG A:ASP323 3.6 17.6 0.5 N B:THR28 3.6 13.5 1.0 CD B:GLU35 3.7 19.2 1.0 C B:THR26 3.8 16.2 1.0 OE2 B:GLU35 3.9 21.3 1.0 OG1 B:THR26 4.0 18.1 1.0 CA B:VAL27 4.0 14.6 1.0 O B:HOH941 4.1 24.0 1.0 C B:VAL27 4.1 14.6 1.0 N B:VAL27 4.2 14.9 1.0 OD1 A:ASP323 4.2 19.7 0.5 CB B:THR28 4.3 15.3 1.0 O B:HOH877 4.3 36.5 1.0 CA B:THR28 4.5 13.9 1.0 O A:HOH743 4.5 28.7 0.5 O B:HOH1167 4.5 30.3 1.0 CB A:ASP322 4.6 16.5 1.0 CB A:ASP323 4.7 15.6 0.5 CA B:THR26 4.8 15.5 1.0 CB B:THR26 4.9 16.1 1.0 CB A:ASP323 4.9 14.7 0.5 O B:HOH1291 4.9 32.8 1.0

T.Koval, L.Svecova, L.H.Ostergaard, T.Skalova, J.Duskova, J.Hasek, P.Kolenko, K.Fejfarova, J.Stransky, M.Trundova, J.Dohnalek. Trp-His Covalent Adduct in Bilirubin Oxidase Is Crucial For Effective Bilirubin Binding But Has A Minor Role in Electron Transfer. Sci Rep V. 9 13700 2019.
ISSN: ESSN 2045-2322
PubMed: 31548583
DOI: 10.1038/S41598-019-50105-3