Atomistry » Potassium » PDB 6f3o-6h6x » 6gvs
Atomistry »
  Potassium »
    PDB 6f3o-6h6x »
      6gvs »

Potassium in PDB 6gvs: Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+

Protein crystallography data

The structure of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+, PDB code: 6gvs was solved by J.Zarzycki, D.Trudeau, M.Scheffen, T.J.Erb, D.S.Tawfik, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 39.13 / 2.58
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 362.202, 123.183, 165.096, 90.00, 109.25, 90.00
R / Rfree (%) 19.2 / 21

Potassium Binding Sites:

The binding sites of Potassium atom in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ (pdb code 6gvs). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 10 binding sites of Potassium where determined in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+, PDB code: 6gvs:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Potassium binding site 1 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 1 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K602

b:63.3
occ:1.00
 O A:GLU416 2.6 63.8 1.0
O A:LYS332 2.6 60.9 1.0
OE1 A:GLU333 2.7 60.4 1.0
O A:VAL414 2.7 58.6 1.0
OD1 A:ASN298 2.7 60.2 1.0
O A:PHE413 2.8 47.3 1.0
C A:VAL414 3.4 58.1 1.0
CG A:ASN298 3.5 55.7 1.0
ND2 A:ASN298 3.5 53.9 1.0
C A:LYS332 3.6 61.9 1.0
CB A:LYS332 3.7 37.3 1.0
CA A:VAL414 3.7 53.2 1.0
C A:GLU416 3.8 53.5 1.0
CD A:GLU333 3.8 57.7 1.0
C A:PHE413 3.8 51.5 1.0
CB A:GLU333 4.3 46.1 1.0
N A:GLU416 4.3 52.7 1.0
N A:VAL414 4.3 58.0 1.0
CA A:LYS332 4.3 51.8 1.0
N A:GLN415 4.5 54.5 1.0
N A:GLU333 4.6 55.6 1.0
C A:GLN415 4.6 63.1 1.0
CG A:GLU333 4.6 43.4 1.0
CA A:GLU416 4.7 52.8 1.0
N A:GLU417 4.7 45.8 1.0
OE2 A:GLU333 4.7 63.6 1.0
CA A:GLU417 4.8 39.0 1.0
CA A:GLU333 4.9 55.0 1.0
CB A:ASN298 4.9 50.1 1.0
CG A:LYS332 5.0 49.5 1.0

Potassium binding site 2 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 2 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K602

b:64.9
occ:1.00
 O B:GLU416 2.5 75.1 1.0
O B:PHE413 2.5 61.3 1.0
O B:VAL414 2.7 71.7 1.0
OD1 B:ASN298 2.9 58.7 1.0
OE1 B:GLU333 2.9 58.7 1.0
O B:LYS332 2.9 62.2 1.0
C B:VAL414 3.4 64.2 1.0
C B:GLU416 3.7 62.4 1.0
C B:PHE413 3.7 56.8 1.0
CG B:ASN298 3.7 50.4 1.0
CB B:LYS332 3.8 45.2 1.0
C B:LYS332 3.8 54.5 1.0
ND2 B:ASN298 3.8 45.5 1.0
CA B:VAL414 3.8 54.1 1.0
CD B:GLU333 4.1 60.1 1.0
N B:VAL414 4.2 52.2 1.0
N B:GLU416 4.3 55.2 1.0
CB B:GLU333 4.3 33.4 1.0
CA B:LYS332 4.4 51.6 1.0
N B:GLN415 4.4 64.3 1.0
C B:GLN415 4.5 61.6 1.0
N B:GLU417 4.5 59.4 1.0
CA B:GLU417 4.6 58.4 1.0
CA B:GLU416 4.6 54.8 1.0
N B:GLU333 4.7 42.1 1.0
CG B:GLU333 4.7 40.8 1.0
N B:LEU418 4.8 72.9 1.0
O B:HOH717 4.8 61.1 1.0
O B:GLN415 4.8 64.7 1.0
CG B:PRO424 4.9 57.8 1.0
CA B:PHE413 4.9 57.6 1.0
CG B:LYS332 5.0 54.3 1.0
CA B:GLN415 5.0 61.9 1.0

Potassium binding site 3 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 3 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K602

b:67.3
occ:1.00
 OE1 C:GLU333 2.4 67.7 1.0
O C:GLU416 2.6 60.1 1.0
OD1 C:ASN298 2.7 56.1 1.0
O C:LYS332 2.8 54.7 1.0
O C:PHE413 2.8 67.0 1.0
O C:VAL414 2.8 51.0 1.0
C C:VAL414 3.6 59.0 1.0
CG C:ASN298 3.6 51.3 1.0
CB C:LYS332 3.6 43.2 1.0
CD C:GLU333 3.7 64.5 1.0
C C:GLU416 3.7 58.2 1.0
ND2 C:ASN298 3.7 46.5 1.0
C C:LYS332 3.7 58.3 1.0
C C:PHE413 3.9 66.0 1.0
CA C:VAL414 3.9 58.8 1.0
N C:GLU416 4.2 58.5 1.0
CA C:LYS332 4.3 50.9 1.0
N C:VAL414 4.4 62.5 1.0
OE2 C:GLU333 4.4 59.4 1.0
CB C:GLU333 4.5 56.1 1.0
N C:GLU417 4.5 51.9 1.0
N C:GLN415 4.5 53.9 1.0
CA C:GLU416 4.6 62.4 1.0
C C:GLN415 4.6 62.0 1.0
CA C:GLU417 4.6 55.2 1.0
CG C:GLU333 4.7 53.8 1.0
N C:GLU333 4.7 52.8 1.0
CG C:LYS332 4.8 47.1 1.0
N C:LEU418 4.9 50.6 1.0
CD C:LYS332 4.9 51.8 1.0

Potassium binding site 4 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 4 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
D:K602

b:76.5
occ:1.00
 O D:LYS332 2.5 76.9 1.0
OE1 D:GLU333 2.5 67.7 1.0
OD1 D:ASN298 2.8 76.1 1.0
O D:GLU416 2.9 73.4 1.0
O D:PHE413 3.0 66.3 1.0
O D:VAL414 3.1 73.7 1.0
CB D:LYS332 3.3 41.4 1.0
C D:LYS332 3.4 58.6 1.0
CG D:ASN298 3.7 66.6 1.0
CD D:GLU333 3.8 66.0 1.0
C D:VAL414 3.8 71.4 1.0
C D:GLU416 3.9 69.5 1.0
ND2 D:ASN298 3.9 66.7 1.0
CA D:LYS332 4.0 47.0 1.0
CA D:VAL414 4.1 64.3 1.0
C D:PHE413 4.1 67.2 1.0
CB D:GLU333 4.2 50.5 1.0
N D:GLU333 4.4 50.4 1.0
N D:GLU416 4.5 74.7 1.0
CG D:GLU333 4.6 60.6 1.0
CG D:LYS332 4.6 52.8 1.0
N D:VAL414 4.6 63.2 1.0
OE2 D:GLU333 4.7 65.0 1.0
N D:GLU417 4.7 67.8 1.0
CA D:GLU417 4.7 73.6 1.0
N D:LEU418 4.7 76.9 1.0
CD D:LYS332 4.8 61.0 1.0
CA D:GLU333 4.8 50.6 1.0
N D:GLN415 4.8 73.6 1.0
CA D:GLU416 4.9 71.8 1.0

Potassium binding site 5 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 5 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
E:K602

b:60.6
occ:1.00
 OE1 E:GLU333 2.5 54.0 1.0
O E:VAL414 2.6 49.0 1.0
O E:GLU416 2.6 55.6 1.0
O E:PHE413 2.6 46.7 1.0
OD1 E:ASN298 2.7 50.6 1.0
O E:LYS332 2.8 57.3 1.0
C E:VAL414 3.4 47.4 1.0
CG E:ASN298 3.5 51.1 1.0
ND2 E:ASN298 3.6 43.6 1.0
C E:LYS332 3.6 43.5 1.0
CD E:GLU333 3.7 48.2 1.0
CB E:LYS332 3.7 30.8 1.0
C E:GLU416 3.7 44.5 1.0
CA E:VAL414 3.7 48.9 1.0
C E:PHE413 3.7 50.5 1.0
CB E:GLU333 4.1 35.3 1.0
N E:GLU416 4.2 43.3 1.0
N E:VAL414 4.2 45.1 1.0
CA E:LYS332 4.3 32.6 1.0
N E:GLU333 4.5 49.4 1.0
CG E:GLU333 4.5 43.5 1.0
N E:GLN415 4.5 49.5 1.0
N E:GLU417 4.6 44.7 1.0
OE2 E:GLU333 4.6 50.0 1.0
CA E:GLU416 4.6 46.3 1.0
CA E:GLU417 4.7 43.1 1.0
C E:GLN415 4.7 48.2 1.0
CA E:GLU333 4.8 42.3 1.0
CG E:LYS332 4.9 49.9 1.0
CG E:PRO424 4.9 36.9 1.0
CB E:ASN298 5.0 31.2 1.0
CA E:PHE413 5.0 54.6 1.0

Potassium binding site 6 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 6 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
F:K602

b:70.0
occ:1.00
 O F:VAL414 2.6 76.2 1.0
O F:GLU416 2.8 80.7 1.0
OD1 F:ASN298 2.9 64.1 1.0
O F:PHE413 3.0 81.3 1.0
OE1 F:GLU333 3.0 78.1 1.0
O F:LYS332 3.3 69.9 1.0
CB F:LYS332 3.3 70.7 1.0
C F:VAL414 3.5 80.1 1.0
C F:GLU416 3.8 76.3 1.0
C F:LYS332 3.8 64.7 1.0
CG F:ASN298 3.9 64.3 1.0
CA F:VAL414 4.0 81.6 1.0
C F:PHE413 4.1 85.6 1.0
ND2 F:ASN298 4.2 51.0 1.0
CA F:LYS332 4.2 67.8 1.0
CD F:GLU333 4.2 73.0 1.0
N F:GLU416 4.3 73.8 1.0
CG F:LYS332 4.4 72.7 1.0
N F:GLN415 4.4 66.8 1.0
N F:VAL414 4.5 85.3 1.0
N F:GLU417 4.5 71.0 1.0
CB F:GLU333 4.5 52.2 1.0
C F:GLN415 4.6 75.5 1.0
CA F:GLU417 4.6 76.7 1.0
N F:GLU333 4.6 60.3 1.0
CA F:GLU416 4.7 75.9 1.0
N F:LEU418 4.7 64.8 1.0
CD F:LYS332 4.9 71.4 1.0
CA F:GLN415 4.9 70.5 1.0
CG F:GLU333 5.0 53.1 1.0

Potassium binding site 7 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 7 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 7 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
G:K602

b:51.9
occ:1.00
 O G:GLU416 2.6 61.1 1.0
OE1 G:GLU333 2.7 37.2 1.0
O G:PHE413 2.7 43.7 1.0
O G:LYS332 2.8 51.8 1.0
OD1 G:ASN298 2.8 52.7 1.0
O G:VAL414 3.1 40.5 1.0
CB G:LYS332 3.6 34.8 1.0
C G:LYS332 3.6 38.7 1.0
C G:VAL414 3.7 43.8 1.0
CG G:ASN298 3.8 46.5 1.0
C G:GLU416 3.8 50.5 1.0
CD G:GLU333 3.9 42.8 1.0
C G:PHE413 3.9 45.2 1.0
ND2 G:ASN298 4.0 39.6 1.0
CA G:VAL414 4.0 37.5 1.0
CB G:GLU333 4.2 31.6 1.0
CA G:LYS332 4.2 35.9 1.0
N G:VAL414 4.4 42.6 1.0
N G:GLU416 4.5 50.5 1.0
N G:GLU333 4.5 27.9 1.0
CA G:GLU417 4.5 46.5 1.0
N G:GLU417 4.5 49.5 1.0
CG G:GLU333 4.6 32.2 1.0
N G:GLN415 4.6 43.0 1.0
C G:GLN415 4.7 49.0 1.0
CA G:GLU416 4.7 39.9 1.0
N G:LEU418 4.8 45.0 1.0
OE2 G:GLU333 4.8 40.8 1.0
CG G:LYS332 4.8 39.3 1.0
CA G:GLU333 4.8 45.5 1.0
CD G:LYS332 4.9 43.2 1.0
CG G:PRO424 5.0 36.4 1.0

Potassium binding site 8 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 8 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 8 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
H:K602

b:58.5
occ:1.00
 O H:GLU416 2.4 54.0 1.0
O H:PHE413 2.7 60.2 1.0
OD1 H:ASN298 2.7 54.0 1.0
OE1 H:GLU333 2.9 63.8 1.0
O H:LYS332 2.9 43.7 1.0
O H:VAL414 3.0 57.9 1.0
C H:GLU416 3.5 52.4 1.0
CG H:ASN298 3.6 50.2 1.0
C H:VAL414 3.6 54.7 1.0
CB H:LYS332 3.7 30.7 1.0
ND2 H:ASN298 3.8 45.3 1.0
C H:LYS332 3.8 43.3 1.0
C H:PHE413 3.8 54.6 1.0
CA H:VAL414 4.0 53.5 1.0
N H:GLU416 4.0 54.5 1.0
CD H:GLU333 4.0 51.8 1.0
CA H:LYS332 4.3 44.4 1.0
N H:VAL414 4.4 59.2 1.0
CA H:GLU416 4.4 45.7 1.0
N H:GLU417 4.4 41.6 1.0
CB H:GLU333 4.4 49.5 1.0
N H:GLN415 4.5 50.2 1.0
CA H:GLU417 4.6 39.9 1.0
C H:GLN415 4.7 57.5 1.0
N H:GLU333 4.7 41.8 1.0
CG H:GLU333 4.8 39.4 1.0
N H:LEU418 4.9 46.9 1.0
OE2 H:GLU333 4.9 59.9 1.0
CG H:LYS332 4.9 35.0 1.0
CA H:PHE413 4.9 56.6 1.0
CD H:LYS332 5.0 48.0 1.0
CB H:ASN298 5.0 42.1 1.0

Potassium binding site 9 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 9 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 9 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
I:K602

b:58.2
occ:1.00
 OE1 I:GLU333 2.4 71.6 1.0
O I:GLU416 2.6 58.1 1.0
O I:LYS332 2.6 58.4 1.0
O I:VAL414 2.7 61.4 1.0
O I:PHE413 2.7 67.9 1.0
OD1 I:ASN298 3.0 41.8 1.0
C I:VAL414 3.4 65.0 1.0
C I:LYS332 3.6 48.6 1.0
CD I:GLU333 3.7 55.9 1.0
CB I:LYS332 3.7 34.8 1.0
C I:GLU416 3.7 52.2 1.0
CA I:VAL414 3.7 56.1 1.0
C I:PHE413 3.8 59.6 1.0
CG I:ASN298 3.9 47.6 1.0
ND2 I:ASN298 4.1 42.3 1.0
N I:GLU416 4.2 51.1 1.0
CB I:GLU333 4.2 48.4 1.0
N I:VAL414 4.2 52.7 1.0
CA I:LYS332 4.3 45.3 1.0
N I:GLN415 4.4 56.6 1.0
OE2 I:GLU333 4.5 52.4 1.0
CG I:GLU333 4.6 40.7 1.0
N I:GLU417 4.6 48.9 1.0
N I:GLU333 4.6 50.1 1.0
CA I:GLU416 4.6 44.7 1.0
C I:GLN415 4.6 55.8 1.0
CA I:GLU417 4.7 43.2 1.0
CG I:LYS332 4.9 36.3 1.0
CA I:GLU333 4.9 56.1 1.0
N I:LEU418 4.9 41.8 1.0
CG I:PRO424 4.9 37.5 1.0
CA I:GLN415 5.0 55.2 1.0

Potassium binding site 10 out of 10 in 6gvs

Go back to Potassium Binding Sites List in 6gvs
Potassium binding site 10 out of 10 in the Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 10 of Engineered Glycolyl-Coa Reductase Comprising 8 Mutations with Bound Nadp+ within 5.0Å range:
probe atom residue distance (Å) B Occ
J:K602

b:64.0
occ:1.00
 O J:GLU416 2.4 52.3 1.0
OD1 J:ASN298 2.5 51.6 1.0
O J:VAL414 2.8 53.5 1.0
O J:PHE413 2.8 49.3 1.0
OE1 J:GLU333 2.9 60.2 1.0
O J:LYS332 3.2 65.4 1.0
CG J:ASN298 3.4 48.8 1.0
C J:GLU416 3.5 46.1 1.0
C J:VAL414 3.5 54.1 1.0
CB J:LYS332 3.6 50.9 1.0
ND2 J:ASN298 3.7 35.8 1.0
C J:PHE413 3.9 50.4 1.0
C J:LYS332 4.0 53.5 1.0
CA J:VAL414 4.0 49.6 1.0
CD J:GLU333 4.1 56.4 1.0
CA J:GLU417 4.2 55.2 1.0
N J:GLU417 4.2 49.8 1.0
N J:GLU416 4.2 54.8 1.0
C J:GLN415 4.4 56.7 1.0
CA J:LYS332 4.4 50.1 1.0
N J:GLN415 4.5 58.8 1.0
CA J:GLU416 4.5 44.2 1.0
N J:VAL414 4.5 49.4 1.0
CG J:LYS332 4.6 52.7 1.0
O J:GLN415 4.6 58.6 1.0
N J:LEU418 4.6 51.3 1.0
CB J:GLU333 4.7 32.1 1.0
CB J:ASN298 4.9 51.4 1.0
N J:GLU333 4.9 54.2 1.0
OE2 J:GLU333 4.9 51.9 1.0
CA J:GLN415 4.9 62.1 1.0
CG J:GLU333 5.0 43.9 1.0
C J:GLU417 5.0 54.0 1.0
CD J:LYS332 5.0 59.0 1.0

Reference:

D.L.Trudeau, C.Edlich-Muth, J.Zarzycki, M.Scheffen, M.Goldsmith, O.Khersonsky, Z.Avizemer, S.J.Fleishman, C.A.R.Cotton, T.J.Erb, D.S.Tawfik, A.Bar-Even. Design and in Vitro Realization of Carbon-Conserving Photorespiration. Proc. Natl. Acad. Sci. V. 115 11455 2018U.S.A..
ISSN: ESSN 1091-6490
PubMed: 30459276
DOI: 10.1073/PNAS.1812605115
Page generated: Mon Aug 12 16:13:58 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy