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Potassium in PDB 6dpj: Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C, PDB code: 6dpj was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.41 / 1.55
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.884, 37.549, 62.400, 90.00, 97.23, 90.00
R / Rfree (%) 17 / 21.1

Other elements in 6dpj:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C also contains other interesting chemical elements:

Manganese (Mn) 2 atoms
Iodine (I) 4 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C (pdb code 6dpj). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C, PDB code: 6dpj:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 6dpj

Go back to Potassium Binding Sites List in 6dpj
Potassium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K203

b:26.8
occ:0.30
O A:TYR193 2.5 94.0 1.0
O A:HOH345 2.7 34.3 0.5
O b:HOH101 2.7 35.0 1.0
OP2 b:G6 2.9 30.1 1.0
OD1 A:ASP192 3.1 34.9 1.0
O A:HOH317 3.3 29.3 1.0
C A:TYR193 3.6 72.5 1.0
HB2 A:TYR193 3.7 48.7 1.0
H A:TYR193 3.7 45.5 1.0
OD2 A:ASP192 3.9 36.8 1.0
P b:G6 3.9 28.5 1.0
CG A:ASP192 3.9 35.9 1.0
MN A:MN201 3.9 28.9 1.0
O5' b:G6 4.2 34.9 1.0
OP1 b:G6 4.2 28.1 1.0
C A:GLY194 4.2 67.0 1.0
N A:TYR193 4.3 37.9 1.0
CA A:TYR193 4.3 53.1 1.0
CB A:TYR193 4.4 40.5 1.0
O A:GLY194 4.6 74.7 1.0
HB3 A:TYR193 4.6 48.7 1.0
N A:GLY194 4.6 65.3 1.0
CA A:GLY194 4.8 66.5 1.0

Potassium binding site 2 out of 2 in 6dpj

Go back to Potassium Binding Sites List in 6dpj
Potassium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with An Rna/Dna Hybrid: Reaction in 4 Mm MN2+ and 200 Mm K+ For 80 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K101

b:46.0
occ:0.60
O C:HOH211 2.0 30.3 0.5
O6 C:DG5 2.6 34.7 1.0
O C:HOH208 3.2 31.8 1.0
O4 C:DT4 3.3 28.6 1.0
C6 C:DG5 3.7 35.0 1.0
N4 B:C2 3.9 33.1 1.0
C4 C:DT4 4.2 33.0 1.0
N7 C:DG5 4.3 31.0 1.0
C7 C:DT4 4.3 35.2 1.0
C5 C:DG5 4.4 34.0 1.0
N6 B:A1 4.4 32.5 1.0
N6 B:A3 4.4 29.1 1.0
O C:HOH201 4.4 52.8 1.0
C5 C:DT4 4.7 34.2 1.0
N1 C:DG5 4.8 35.8 1.0
O B:HOH103 4.9 49.7 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Aug 12 15:53:44 2024

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