Potassium in PDB 6dph: Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C
Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C
All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C:
3.1.26.4;
Protein crystallography data
The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C, PDB code: 6dph
was solved by
N.L.Samara,
W.Yang,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
23.20 /
1.34
|
Space group
|
C 1 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
80.867,
37.063,
61.789,
90.00,
96.33,
90.00
|
R / Rfree (%)
|
16 /
19.2
|
Other elements in 6dph:
The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C also contains other interesting chemical elements:
Potassium Binding Sites:
The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C
(pdb code 6dph). This binding sites where shown within
5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the
Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C, PDB code: 6dph:
Jump to Potassium binding site number:
1;
2;
3;
Potassium binding site 1 out
of 3 in 6dph
Go back to
Potassium Binding Sites List in 6dph
Potassium binding site 1 out
of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:K203
b:17.2
occ:0.40
|
MG
|
A:MG201
|
1.8
|
16.2
|
0.6
|
OD2
|
A:ASP71
|
2.4
|
25.6
|
0.6
|
O
|
A:VAL72
|
2.4
|
19.9
|
1.0
|
OP1
|
b:C5
|
2.5
|
19.2
|
1.0
|
OD1
|
A:ASP71
|
2.5
|
18.8
|
0.4
|
OD2
|
A:ASP71
|
2.7
|
14.1
|
0.4
|
OP2
|
b:G6
|
2.8
|
28.0
|
1.0
|
CG
|
A:ASP71
|
3.0
|
13.6
|
0.4
|
O
|
A:HOH331
|
3.0
|
23.1
|
0.6
|
CG
|
A:ASP71
|
3.0
|
22.1
|
0.6
|
OD1
|
A:ASP71
|
3.3
|
22.0
|
0.6
|
H
|
A:TYR193
|
3.3
|
45.9
|
1.0
|
OD1
|
A:ASP192
|
3.3
|
34.2
|
1.0
|
HD1
|
A:TYR193
|
3.4
|
37.7
|
1.0
|
HB2
|
A:TYR193
|
3.6
|
83.1
|
1.0
|
C
|
A:VAL72
|
3.6
|
19.2
|
1.0
|
O
|
b:HOH103
|
3.7
|
34.1
|
1.0
|
P
|
b:C5
|
3.7
|
18.6
|
1.0
|
HA2
|
A:GLY73
|
3.7
|
23.4
|
1.0
|
O
|
A:HOH424
|
3.9
|
31.4
|
0.4
|
C5'
|
b:C5
|
3.9
|
18.1
|
1.0
|
P
|
b:G6
|
4.0
|
21.3
|
1.0
|
HA
|
A:ASP192
|
4.0
|
30.6
|
1.0
|
H
|
A:VAL72
|
4.0
|
18.7
|
1.0
|
O5'
|
b:C5
|
4.1
|
17.9
|
1.0
|
N
|
A:TYR193
|
4.1
|
38.3
|
1.0
|
HG13
|
A:VAL72
|
4.1
|
24.1
|
1.0
|
K
|
A:K204
|
4.2
|
15.6
|
0.4
|
MG
|
A:MG202
|
4.2
|
23.8
|
0.6
|
HB3
|
A:ASP71
|
4.2
|
27.1
|
0.6
|
HG12
|
A:VAL72
|
4.3
|
24.1
|
1.0
|
OP1
|
b:G6
|
4.3
|
21.6
|
1.0
|
CB
|
A:ASP71
|
4.3
|
22.6
|
0.6
|
CD1
|
A:TYR193
|
4.3
|
31.4
|
1.0
|
N
|
A:VAL72
|
4.4
|
15.6
|
1.0
|
CB
|
A:ASP71
|
4.5
|
12.5
|
0.4
|
CB
|
A:TYR193
|
4.5
|
69.3
|
1.0
|
CA
|
A:GLY73
|
4.5
|
19.5
|
1.0
|
N
|
A:GLY73
|
4.5
|
18.4
|
1.0
|
OP2
|
b:C5
|
4.5
|
20.4
|
1.0
|
CG
|
A:ASP192
|
4.5
|
66.5
|
1.0
|
O3'
|
b:C5
|
4.6
|
17.2
|
1.0
|
CA
|
A:VAL72
|
4.6
|
17.6
|
1.0
|
CG1
|
A:VAL72
|
4.7
|
20.1
|
1.0
|
HA3
|
A:GLY73
|
4.8
|
23.4
|
1.0
|
HB3
|
A:ASP71
|
4.8
|
15.0
|
0.4
|
CA
|
A:ASP192
|
4.8
|
25.5
|
1.0
|
CA
|
A:TYR193
|
4.9
|
41.7
|
1.0
|
CG
|
A:TYR193
|
4.9
|
41.9
|
1.0
|
HB2
|
A:ASP71
|
4.9
|
27.1
|
0.6
|
O3'
|
B:U4
|
4.9
|
18.7
|
1.0
|
O
|
b:HOH104
|
4.9
|
38.4
|
1.0
|
HB2
|
A:ASP71
|
5.0
|
15.0
|
0.4
|
C4'
|
b:C5
|
5.0
|
18.0
|
1.0
|
|
Potassium binding site 2 out
of 3 in 6dph
Go back to
Potassium Binding Sites List in 6dph
Potassium binding site 2 out
of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:K204
b:15.6
occ:0.40
|
MG
|
A:MG202
|
0.6
|
23.8
|
0.6
|
OD1
|
A:ASP71
|
2.0
|
22.0
|
0.6
|
OD1
|
A:ASP132
|
2.5
|
17.6
|
1.0
|
OD2
|
A:ASP71
|
2.5
|
14.1
|
0.4
|
OP1
|
b:C5
|
2.7
|
19.2
|
1.0
|
OE1
|
A:GLU109
|
2.7
|
28.2
|
1.0
|
OD2
|
A:ASP132
|
2.8
|
22.2
|
1.0
|
CG
|
A:ASP132
|
2.9
|
17.5
|
1.0
|
CG
|
A:ASP71
|
2.9
|
22.1
|
0.6
|
O
|
A:HOH358
|
2.9
|
19.7
|
1.0
|
O3'
|
B:U4
|
3.1
|
18.7
|
1.0
|
OE2
|
A:GLU109
|
3.3
|
22.0
|
1.0
|
CD
|
A:GLU109
|
3.4
|
42.6
|
1.0
|
OD2
|
A:ASP71
|
3.4
|
25.6
|
0.6
|
O
|
b:HOH103
|
3.4
|
34.1
|
1.0
|
P
|
b:C5
|
3.5
|
18.6
|
1.0
|
H
|
A:VAL72
|
3.5
|
18.7
|
1.0
|
HA
|
A:ASP71
|
3.6
|
19.7
|
1.0
|
CG
|
A:ASP71
|
3.7
|
13.6
|
0.4
|
HG12
|
A:VAL72
|
3.7
|
24.1
|
1.0
|
HG21
|
A:ILE189
|
3.8
|
28.0
|
1.0
|
MG
|
A:MG201
|
3.9
|
16.2
|
0.6
|
H
|
A:ASP132
|
4.1
|
17.2
|
1.0
|
CB
|
A:ASP71
|
4.1
|
22.6
|
0.6
|
K
|
A:K203
|
4.2
|
17.2
|
0.4
|
HB2
|
A:ASP71
|
4.3
|
27.1
|
0.6
|
CB
|
A:ASP132
|
4.3
|
17.8
|
1.0
|
CA
|
A:ASP71
|
4.3
|
17.1
|
0.6
|
CA
|
A:ASP71
|
4.3
|
16.4
|
0.4
|
N
|
A:VAL72
|
4.4
|
15.6
|
1.0
|
C3'
|
B:U4
|
4.4
|
20.8
|
1.0
|
CB
|
A:ASP71
|
4.4
|
12.5
|
0.4
|
HB2
|
A:ASP71
|
4.4
|
15.0
|
0.4
|
HG22
|
A:ILE189
|
4.4
|
28.0
|
1.0
|
C5'
|
b:C5
|
4.5
|
18.1
|
1.0
|
O5'
|
b:C5
|
4.5
|
17.9
|
1.0
|
HB2
|
A:SER133
|
4.6
|
17.5
|
1.0
|
O2'
|
B:U4
|
4.6
|
19.7
|
1.0
|
CG2
|
A:ILE189
|
4.6
|
23.4
|
1.0
|
C4'
|
B:U4
|
4.6
|
19.5
|
1.0
|
HB3
|
A:ASP132
|
4.6
|
21.3
|
1.0
|
OD1
|
A:ASP71
|
4.7
|
18.8
|
0.4
|
OP2
|
b:C5
|
4.7
|
20.4
|
1.0
|
CG1
|
A:VAL72
|
4.7
|
20.1
|
1.0
|
N
|
A:ASP132
|
4.8
|
14.4
|
1.0
|
O
|
A:VAL72
|
4.9
|
19.9
|
1.0
|
CG
|
A:GLU109
|
4.9
|
23.3
|
1.0
|
HB3
|
A:ASP71
|
4.9
|
27.1
|
0.6
|
HD13
|
A:ILE189
|
4.9
|
23.7
|
1.0
|
HB2
|
A:ASP132
|
4.9
|
21.3
|
1.0
|
O
|
A:VAL70
|
4.9
|
16.1
|
1.0
|
CA
|
A:ASP132
|
4.9
|
15.5
|
1.0
|
C
|
A:ASP71
|
4.9
|
14.9
|
1.0
|
HG13
|
A:VAL72
|
5.0
|
24.1
|
1.0
|
|
Potassium binding site 3 out
of 3 in 6dph
Go back to
Potassium Binding Sites List in 6dph
Potassium binding site 3 out
of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 E188A in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MN2+ and 200 Mm K+ For 120 S at 21 C within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:K101
b:34.5
occ:0.50
|
O
|
C:HOH214
|
2.5
|
24.1
|
0.5
|
O6
|
C:DG5
|
2.6
|
24.4
|
1.0
|
O
|
C:HOH213
|
3.2
|
27.4
|
1.0
|
O4
|
C:DT4
|
3.2
|
21.8
|
1.0
|
C6
|
C:DG5
|
3.7
|
23.6
|
1.0
|
N4
|
B:C2
|
3.9
|
26.8
|
1.0
|
N7
|
C:DG5
|
4.1
|
22.1
|
1.0
|
C4
|
C:DT4
|
4.1
|
17.9
|
1.0
|
C7
|
C:DT4
|
4.2
|
21.4
|
1.0
|
C5
|
C:DG5
|
4.2
|
22.5
|
1.0
|
N6
|
B:A1
|
4.3
|
28.0
|
1.0
|
O
|
B:HOH107
|
4.3
|
44.9
|
1.0
|
N6
|
B:A3
|
4.4
|
19.7
|
1.0
|
O
|
C:HOH202
|
4.4
|
29.8
|
1.0
|
C5
|
C:DT4
|
4.6
|
20.5
|
1.0
|
O
|
B:HOH103
|
4.7
|
53.6
|
1.0
|
N1
|
C:DG5
|
4.8
|
24.6
|
1.0
|
O4
|
C:DT6
|
5.0
|
29.6
|
1.0
|
|
Reference:
N.L.Samara,
W.Yang.
Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Aug 12 15:53:40 2024
|