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Potassium in PDB 6dp5: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C, PDB code: 6dp5 was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.01 / 1.43
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.899, 37.505, 62.254, 90.00, 96.48, 90.00
R / Rfree (%) 15.3 / 17.6

Other elements in 6dp5:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Iodine (I) 4 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C (pdb code 6dp5). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C, PDB code: 6dp5:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in 6dp5

Go back to Potassium Binding Sites List in 6dp5
Potassium binding site 1 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K203

b:30.3
occ:0.10
O b:HOH102 0.7 30.8 0.9
H A:ARG195 2.2 48.6 0.9
O A:HOH301 2.8 33.5 0.3
OD1 A:ASP192 2.9 26.8 1.0
N A:ARG195 2.9 40.5 0.9
H A:GLY194 3.0 35.5 1.0
OP2 b:G6 3.0 24.3 1.0
O A:HOH330 3.0 41.3 1.0
NZ A:LYS196 3.1 35.4 0.7
OD2 A:ASP192 3.3 28.3 1.0
HB2 A:ARG195 3.3 51.9 0.9
N A:GLY194 3.4 29.6 1.0
CG A:ASP192 3.4 27.1 1.0
H A:TYR193 3.4 28.1 1.0
N A:LYS196 3.4 47.3 0.7
O A:HOH308 3.5 23.2 1.0
OP3 b:C5 3.5 26.3 0.7
CG A:LYS196 3.5 38.2 0.7
HB2 A:TYR193 3.7 38.2 1.0
CA A:ARG195 3.7 43.9 0.9
C A:GLY194 3.8 45.1 1.0
HA2 A:GLY194 3.9 40.2 1.0
CA A:GLY194 3.9 33.5 1.0
C A:ARG195 3.9 57.7 0.9
CB A:ARG195 3.9 43.2 0.9
MG A:MG201 3.9 22.4 1.0
HG3 A:ARG195 4.0 47.3 0.9
N A:TYR193 4.0 23.4 1.0
C A:TYR193 4.0 37.0 1.0
CB A:LYS196 4.1 41.6 0.7
CD A:LYS196 4.1 36.0 0.7
CE A:LYS196 4.1 35.2 0.7
P b:G6 4.1 24.1 1.0
CA A:TYR193 4.3 27.1 1.0
CB A:TYR193 4.4 31.9 1.0
CA A:LYS196 4.4 50.4 0.7
O5' b:G6 4.4 27.8 1.0
CG A:ARG195 4.5 39.4 0.9
OP1 b:G6 4.6 23.7 1.0
HA A:ARG195 4.6 52.7 0.9
HB3 A:TYR193 4.7 38.2 1.0
HB3 A:ARG195 4.8 51.9 0.9
HD2 A:ARG195 4.8 55.7 0.9
HA3 A:GLY194 4.8 40.2 1.0
CB A:ASP192 4.9 26.0 1.0
O A:TYR193 4.9 36.5 1.0
P b:C5 4.9 27.2 0.7
O A:GLY194 5.0 47.0 1.0
C A:ASP192 5.0 25.7 1.0
O A:ARG195 5.0 67.9 0.9

Potassium binding site 2 out of 3 in 6dp5

Go back to Potassium Binding Sites List in 6dp5
Potassium binding site 2 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K204

b:37.8
occ:0.35
O A:HOH303 0.9 36.1 0.4
OP2 b:C5 2.4 31.9 0.3
OP2 b:C5 2.6 24.3 0.7
O B:HOH105 2.9 41.0 1.0
O A:HOH331 3.0 25.1 1.0
OE2 A:GLU188 3.1 53.0 1.0
O A:HOH362 3.2 23.0 1.0
C5' B:U4 3.3 22.6 0.3
P b:C5 3.4 17.5 0.3
OD2 A:ASP132 3.5 34.0 1.0
HG21 A:THR183 3.5 34.6 1.0
OE1 A:GLU188 3.6 74.7 1.0
O b:HOH101 3.6 41.0 1.0
C5' B:U4 3.6 31.3 0.7
P b:C5 3.6 27.2 0.7
CD A:GLU188 3.7 61.7 1.0
HG1 A:THR183 3.8 29.2 1.0
O3' B:U4 3.8 16.8 0.3
OP3 b:C5 3.8 26.3 0.7
OP1 b:C5 3.9 31.0 0.3
C3' B:U4 4.0 25.0 0.3
CE A:LYS196 4.1 35.2 0.7
NZ A:LYS196 4.1 35.4 0.7
CG A:ASP132 4.2 28.8 1.0
OP1 b:C5 4.2 27.9 0.7
C4' B:U4 4.2 20.9 0.3
O A:HOH301 4.2 33.5 0.3
O5' B:U4 4.3 31.6 0.7
HB2 A:ASP132 4.4 24.7 1.0
O5' B:U4 4.4 21.0 0.3
OP1 B:U4 4.4 29.5 1.0
CG2 A:THR183 4.4 28.9 1.0
OG1 A:THR183 4.5 24.3 1.0
HG23 A:THR183 4.5 34.6 1.0
MG A:MG201 4.5 22.4 1.0
P B:U4 4.7 23.9 1.0
CB A:ASP132 4.8 20.6 1.0
C4' B:U4 4.8 25.9 0.7
OD1 A:ASP132 4.8 24.5 1.0
O5' b:C5 4.8 19.3 0.3
HA A:GLU188 4.8 30.0 1.0
OP2 B:U4 4.8 26.7 1.0
C3' B:U4 4.9 22.9 0.7
O5' b:C5 4.9 27.1 0.7
MG A:MG202 4.9 21.5 1.0
HB3 A:ASP132 4.9 24.7 1.0

Potassium binding site 3 out of 3 in 6dp5

Go back to Potassium Binding Sites List in 6dp5
Potassium binding site 3 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 40 Mm MG2+ and 75 Mm K+ For 40 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K101

b:65.8
occ:0.50
O C:HOH207 2.7 25.8 0.6
O6 C:DG5 2.8 25.9 1.0
O B:HOH108 3.1 50.1 1.0
O C:HOH206 3.1 33.7 1.0
O C:HOH205 3.8 41.1 1.0
C6 C:DG5 3.8 22.6 1.0
O4 C:DT4 3.9 23.4 1.0
N4 B:C2 4.1 27.2 1.0
N6 B:A1 4.1 27.4 1.0
N7 C:DG5 4.2 23.7 1.0
C5 C:DG5 4.4 20.9 1.0
C7 C:DT4 4.5 23.5 1.0
O B:HOH106 4.6 46.0 1.0
C4 C:DT4 4.6 20.4 1.0
O4 C:DT6 4.9 28.8 1.0
C5 C:DT4 5.0 21.5 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Aug 12 15:51:43 2024

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