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Potassium in PDB 6do9: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C, PDB code: 6do9 was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.86 / 1.36
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.301, 37.773, 62.509, 90.00, 96.22, 90.00
R / Rfree (%) 15.4 / 17.5

Other elements in 6do9:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Iodine (I) 4 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C (pdb code 6do9). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C, PDB code: 6do9:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in 6do9

Go back to Potassium Binding Sites List in 6do9
Potassium binding site 1 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K202

b:28.2
occ:0.25
O A:HOH343 0.8 26.6 0.4
H A:ARG195 2.3 59.7 0.7
O A:HOH308 2.5 27.3 0.7
O A:HOH354 2.6 31.3 0.6
OD1 A:ASP192 2.9 29.3 1.0
OP2 b:G6 2.9 23.0 1.0
OP3 b:C5 3.0 65.9 0.3
N A:ARG195 3.1 49.7 0.7
HB2 A:ARG195 3.1 60.8 0.7
O A:HOH316 3.3 22.9 1.0
CG A:LYS196 3.3 50.8 0.7
H A:GLY194 3.3 41.5 1.0
OD2 A:ASP192 3.5 27.7 1.0
N A:LYS196 3.5 60.1 0.7
CG A:ASP192 3.5 25.3 1.0
H A:TYR193 3.6 31.2 1.0
N A:GLY194 3.6 34.5 1.0
MG A:MG215 3.7 20.9 1.0
HB2 A:TYR193 3.8 37.3 1.0
CA A:ARG195 3.8 52.9 0.7
CD A:LYS196 3.8 49.7 0.7
CB A:ARG195 3.9 50.7 0.7
C A:ARG195 3.9 50.3 0.7
CB A:LYS196 3.9 53.0 0.7
CE A:LYS196 3.9 60.2 0.7
P b:G6 4.1 21.5 1.0
C A:GLY194 4.1 55.0 1.0
HA2 A:GLY194 4.1 45.1 1.0
N A:TYR193 4.1 26.0 1.0
CA A:GLY194 4.2 37.6 1.0
C A:TYR193 4.2 43.1 1.0
CA A:LYS196 4.4 85.8 0.7
O5' b:G6 4.4 23.6 1.0
P b:C5 4.4 78.0 0.3
CA A:TYR193 4.5 29.4 1.0
OP1 b:G6 4.5 22.8 1.0
CB A:TYR193 4.5 31.1 1.0
HB3 A:ARG195 4.5 60.8 0.7
OP1 b:C5 4.6 46.1 0.2
HG3 A:ARG195 4.6 52.7 0.7
HD2 A:ARG195 4.6 55.9 0.7
HA A:ARG195 4.7 63.4 0.7
HB3 A:TYR193 4.8 37.3 1.0
O5' b:C5 4.8 68.5 0.3
OE1 A:GLU188 4.8 87.9 1.0
CG A:ARG195 4.8 43.9 0.7
O A:ARG195 4.8 68.3 0.7
CB A:ASP192 5.0 26.6 1.0

Potassium binding site 2 out of 3 in 6do9

Go back to Potassium Binding Sites List in 6do9
Potassium binding site 2 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K203

b:24.9
occ:0.25
O A:HOH345 0.9 29.3 0.6
OP2 b:C5 2.5 62.1 0.4
O B:HOH310 2.6 32.0 1.0
OP2 b:C5 2.6 30.7 0.2
OP2 b:C5 2.7 19.1 0.3
C5' B:U4 2.9 18.8 0.2
C5' B:U4 2.9 19.2 0.4
OD2 A:ASP132 3.3 30.4 1.0
OE2 A:GLU188 3.3 58.1 1.0
C5' B:U4 3.4 26.5 0.3
O A:HOH330 3.4 24.8 1.0
P b:C5 3.4 75.1 0.2
O A:HOH301 3.4 36.9 1.0
O3' B:U4 3.4 39.5 0.2
P b:C5 3.4 18.6 0.4
O A:HOH335 3.5 22.3 1.0
O3' B:U4 3.5 17.7 0.4
C3' B:U4 3.7 17.6 0.2
C3' B:U4 3.7 21.4 0.4
HG21 A:THR183 3.7 30.0 1.0
P b:C5 3.7 78.0 0.3
C4' B:U4 3.7 17.5 0.2
C4' B:U4 3.8 19.5 0.4
HG1 A:THR183 3.8 26.8 1.0
OP1 b:C5 3.9 46.1 0.2
O5' B:U4 4.0 36.7 0.3
O5' B:U4 4.0 18.6 0.2
NZ A:LYS196 4.0 83.0 0.7
O5' B:U4 4.0 19.2 0.4
OP1 b:C5 4.1 19.1 0.3
CG A:ASP132 4.1 21.2 1.0
CD A:GLU188 4.1 74.6 1.0
OP1 b:C5 4.1 39.6 0.4
OP3 b:C5 4.1 65.9 0.3
OE1 A:GLU188 4.2 87.9 1.0
O A:HOH308 4.3 27.3 0.7
OP1 B:U4 4.4 23.8 1.0
OP2 B:U4 4.4 21.9 1.0
P B:U4 4.5 20.4 1.0
C4' B:U4 4.5 18.6 0.3
CG2 A:THR183 4.6 25.0 1.0
C3' B:U4 4.6 19.3 0.3
OG1 A:THR183 4.6 22.3 1.0
OD1 A:ASP132 4.7 20.7 1.0
CE A:LYS196 4.7 60.2 0.7
MG A:MG215 4.7 20.9 1.0
HG23 A:THR183 4.7 30.0 1.0
CB A:ASP132 4.8 16.7 1.0
O5' b:C5 4.8 18.4 0.4
MG A:MG201 4.8 21.5 1.0
O5' b:C5 4.9 18.5 0.2
C2' B:U4 4.9 35.0 0.3
O4' B:U4 5.0 29.2 0.2
O4' B:U4 5.0 56.5 0.4

Potassium binding site 3 out of 3 in 6do9

Go back to Potassium Binding Sites List in 6do9
Potassium binding site 3 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 2 Mm MG2+ and 200 Mm K+ For 120 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K101

b:43.8
occ:0.50
O C:HOH215 2.2 24.0 0.5
O6 C:DG5 2.6 28.0 1.0
O C:HOH208 3.1 33.9 1.0
O4 C:DT4 3.3 23.2 1.0
C6 C:DG5 3.6 24.6 1.0
N4 B:C2 4.0 30.7 1.0
N7 C:DG5 4.0 23.6 1.0
C7 C:DT4 4.1 24.9 1.0
O C:HOH204 4.1 39.6 1.0
C4 C:DT4 4.1 22.4 1.0
C5 C:DG5 4.2 23.4 1.0
C5 C:DT4 4.5 23.1 1.0
N6 B:A3 4.5 21.3 1.0
N6 B:A1 4.6 28.5 1.0
O B:HOH308 4.6 41.7 1.0
N1 C:DG5 4.8 26.0 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Aug 12 15:47:09 2024

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