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Potassium in PDB 6dmv: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C, PDB code: 6dmv was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.88 / 1.52
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.422, 37.803, 61.986, 90.00, 96.31, 90.00
R / Rfree (%) 16.1 / 18.2

Other elements in 6dmv:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Iodine (I) 4 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C (pdb code 6dmv). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C, PDB code: 6dmv:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 6dmv

Go back to Potassium Binding Sites List in 6dmv
Potassium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K203

b:40.1
occ:0.35
O A:HOH312 2.6 34.3 1.0
N A:ARG195 2.7 67.2 0.6
OP2 b:G6 2.8 28.6 1.0
O A:ARG195 2.9 64.2 0.6
O A:HOH310 2.9 55.0 1.0
O A:HOH333 3.1 25.5 1.0
OD1 A:ASP192 3.3 37.4 1.0
N A:GLY194 3.4 38.6 0.7
CA A:ARG195 3.4 70.0 0.6
C A:ARG195 3.6 65.8 0.6
CB A:ARG195 3.6 73.2 0.6
C A:GLY194 3.7 64.0 0.7
HB2 A:TYR193 3.7 48.0 1.0
O b:HOH103 3.7 47.0 1.0
MG A:MG201 3.8 23.9 1.0
H A:TYR193 3.8 43.3 1.0
CA A:GLY194 3.8 61.1 0.7
P b:G6 3.9 24.6 1.0
OD2 A:ASP192 4.0 41.4 1.0
CG A:ASP192 4.0 36.7 1.0
C A:TYR193 4.1 50.4 1.0
O5' b:G6 4.2 27.3 1.0
OP1 b:G6 4.2 26.0 1.0
CG A:ARG195 4.3 62.6 0.6
N A:TYR193 4.4 36.0 1.0
CB A:TYR193 4.4 40.0 1.0
CA A:TYR193 4.5 35.9 1.0
HB3 A:TYR193 4.6 48.0 1.0
O A:GLY194 4.8 68.6 0.7
OP1 b:C5 4.9 28.9 1.0
O A:TYR193 5.0 59.8 1.0

Potassium binding site 2 out of 2 in 6dmv

Go back to Potassium Binding Sites List in 6dmv
Potassium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked For 40 S in 2 Mm MG2+ and 200 Mm K+ at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K101

b:41.3
occ:0.50
O C:HOH208 2.1 22.5 0.5
O6 C:DG5 2.6 29.1 1.0
O B:HOH109 3.0 49.9 1.0
O C:HOH206 3.1 30.3 1.0
O4 C:DT4 3.2 26.4 1.0
C6 C:DG5 3.6 29.2 1.0
N7 C:DG5 4.0 25.3 1.0
C4 C:DT4 4.0 21.8 1.0
C7 C:DT4 4.1 27.1 1.0
N4 B:C2 4.1 31.8 1.0
C5 C:DG5 4.2 28.6 1.0
N6 B:A3 4.4 24.7 1.0
O C:HOH201 4.4 34.4 1.0
C5 C:DT4 4.5 26.4 1.0
O B:HOH107 4.5 45.5 1.0
N6 B:A1 4.5 33.8 1.0
N1 C:DG5 4.8 30.8 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Aug 12 15:46:28 2024

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