Potassium in PDB 6d7h: Ctx-M-14 Apoenzyme

All present enzymatic activity of Ctx-M-14 Apoenzyme:
3.5.2.6;

The structure of Ctx-M-14 Apoenzyme, PDB code: 6d7h was solved by M.Kemp, D.Nichols, Y.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.68 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	41.710, 41.710, 232.524, 90.00, 90.00, 120.00
R / Rfree (%)	21 / 26.4

The binding sites of Potassium atom in the Ctx-M-14 Apoenzyme (pdb code 6d7h). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Ctx-M-14 Apoenzyme, PDB code: 6d7h:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Ctx-M-14 Apoenzyme


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Ctx-M-14 Apoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ A:K302 b:23.9 occ:0.71 O A:SER130 2.8 18.8 1.0 OG A:SER130 2.9 18.1 1.0 OG A:SER70 2.9 13.4 1.0 O4 A:PO4301 3.0 18.3 1.0 O A:HOH546 3.0 41.1 1.0 O A:HOH524 3.0 23.4 1.0 OD1 A:ASN132 3.0 13.4 1.0 CB A:SER130 3.5 20.5 1.0 O A:HOH461 3.7 16.8 1.0 C A:SER130 3.7 21.9 1.0 CG A:ASN132 3.8 13.9 1.0 CB A:SER70 4.0 16.1 1.0 ND2 A:ASN132 4.0 15.3 1.0 O A:HOH421 4.1 19.8 1.0 CD2 A:TYR105 4.1 27.8 1.0 NZ A:LYS73 4.1 9.2 1.0 CA A:SER130 4.1 22.7 1.0 P A:PO4301 4.2 22.6 1.0 O3 A:PO4301 4.4 23.2 1.0 O2 A:PO4301 4.5 28.3 1.0 CE A:LYS73 4.5 12.4 1.0 CE2 A:TYR105 4.6 33.0 1.0 N A:ASP131 4.9 17.9 1.0 CG A:TYR105 4.9 29.6 1.0

Potassium binding site 2 out of 4 in the Ctx-M-14 Apoenzyme


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Ctx-M-14 Apoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ A:K303 b:22.2 occ:0.58 O A:ASP176 2.7 20.0 1.0 O A:ILE173 2.8 16.9 1.0 O A:HOH463 2.8 19.3 1.0 O A:HOH559 3.0 33.4 1.0 CG A:PRO67 3.6 13.3 1.0 C A:ASP176 3.7 20.2 1.0 CB A:ASP176 3.7 19.7 1.0 N A:ASP176 3.8 27.6 1.0 C A:ILE173 3.9 19.7 1.0 CG2 A:THR180 3.9 10.1 1.0 N A:ILE173 3.9 10.9 1.0 CA A:ASP176 3.9 25.3 1.0 C A:ALA172 4.0 19.5 1.0 O A:HOH425 4.1 17.9 1.0 O A:HOH543 4.2 37.9 1.0 CB A:PRO67 4.2 11.5 1.0 CA A:ALA172 4.2 16.4 1.0 CD A:PRO67 4.3 12.8 1.0 O A:HOH517 4.4 24.3 1.0 O A:ALA172 4.4 21.0 1.0 O A:ARG178 4.5 12.2 1.0 CA A:ILE173 4.5 18.4 1.0 N A:PRO67 4.6 11.5 1.0 N A:GLY175 4.6 17.6 1.0 CA A:PRO67 4.6 9.7 1.0 C A:GLY175 4.7 18.9 1.0 CG A:ASP176 4.7 14.8 1.0 O A:HOH456 4.7 15.9 1.0 N A:PRO174 4.8 18.7 1.0 N A:PRO177 4.9 18.1 1.0 CA A:PRO174 5.0 20.3 1.0

Potassium binding site 3 out of 4 in the Ctx-M-14 Apoenzyme


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Ctx-M-14 Apoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ A:K304 b:11.3 occ:0.31 O A:HOH548 2.6 33.9 1.0 O A:GLY238 2.7 13.1 1.0 O A:HOH606 2.8 41.1 1.0 O A:HOH485 2.9 28.2 1.0 O A:HOH446 3.4 20.5 1.0 C A:GLY238 3.5 16.3 1.0 N A:ASP240 4.3 17.5 1.0 N A:GLY238 4.3 13.0 1.0 CA A:GLY238 4.3 11.7 1.0 O A:ALA272 4.3 19.2 1.0 OG A:SER274 4.4 17.7 0.5 CA A:ASP240 4.4 21.0 1.0 CA A:GLY242 4.6 16.9 1.0 N A:GLY242 4.7 15.7 1.0 OD1 A:ASP240 5.0 25.9 1.0 C A:ASP240 5.0 18.2 1.0

Potassium binding site 4 out of 4 in the Ctx-M-14 Apoenzyme


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Ctx-M-14 Apoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ A:K305 b:69.4 occ:1.00 NH1 A:ARG61 3.5 33.0 1.0 NH2 A:ARG61 3.8 28.5 1.0 CZ A:ARG61 4.1 29.3 1.0 O A:HOH577 4.4 32.2 1.0

D.A.Nichols, M.T.Kemp, Y.Chen. The Role of Asp-Asp Short Hydrogen Bond in Maintaining Active Site Integrity of Ctx-M Beta-Lactamase To Be Published.