Potassium in PDB 5yja: Crystal Structure of Highly Active Btuo Mutant P287G Without Dehydration

All present enzymatic activity of Crystal Structure of Highly Active Btuo Mutant P287G Without Dehydration:
1.7.3.3; 4.1.1.97;

The structure of Crystal Structure of Highly Active Btuo Mutant P287G Without Dehydration, PDB code: 5yja was solved by T.Hibi, T.Itoh, Y.Nishiya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.68 / 1.65
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	133.017, 144.252, 70.272, 90.00, 90.00, 90.00
R / Rfree (%)	16.4 / 18.7

The binding sites of Potassium atom in the Crystal Structure of Highly Active Btuo Mutant P287G Without Dehydration (pdb code 5yja). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Highly Active Btuo Mutant P287G Without Dehydration, PDB code: 5yja:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Highly Active Btuo Mutant P287G Without Dehydration


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Highly Active Btuo Mutant P287G Without Dehydration within 5.0Å range: probe atom residue distance (Å) B Occ B:K404 b:28.8 occ:1.00 NH1 C:ARG133 2.8 28.6 1.0 O B:SER35 2.9 13.3 1.0 O B:ILE32 2.9 11.5 1.0 HH11 C:ARG133 3.1 34.3 1.0 HH22 C:ARG133 3.4 43.6 1.0 HA B:PRO36 3.4 16.6 1.0 HB B:THR31 3.6 11.0 1.0 HA B:PRO33 3.6 15.5 1.0 CZ C:ARG133 3.7 31.3 1.0 C B:SER35 3.7 11.6 1.0 H B:SER35 3.9 14.9 1.0 NH2 C:ARG133 3.9 36.3 1.0 C B:ILE32 3.9 9.4 1.0 O B:PRO33 4.1 14.5 1.0 H B:ILE32 4.3 13.9 1.0 CA B:PRO36 4.3 13.8 1.0 CA B:PRO33 4.3 12.9 1.0 N B:PRO36 4.4 13.8 1.0 C B:PRO33 4.4 12.9 1.0 N B:SER35 4.5 12.4 1.0 HG1 B:THR31 4.5 12.7 1.0 CB B:THR31 4.5 9.2 1.0 N B:PRO33 4.5 13.2 1.0 O B:HOH649 4.6 11.1 1.0 O B:HOH687 4.7 27.3 1.0 N B:ILE32 4.7 11.6 1.0 HH21 C:ARG133 4.7 43.6 1.0 CA B:SER35 4.7 11.4 1.0 OG1 B:THR31 4.8 10.5 1.0 NE C:ARG133 4.9 31.9 1.0 HD3 C:ARG133 5.0 36.7 1.0 HG21 B:THR31 5.0 12.8 1.0 CA B:ILE32 5.0 11.3 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Highly Active Btuo Mutant P287G Without Dehydration


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Highly Active Btuo Mutant P287G Without Dehydration within 5.0Å range: probe atom residue distance (Å) B Occ C:K403 b:28.8 occ:1.00 NH1 B:ARG133 2.8 30.7 1.0 O C:SER35 2.9 13.2 1.0 O C:ILE32 2.9 11.2 1.0 HH11 B:ARG133 3.2 36.9 1.0 HH22 B:ARG133 3.4 41.1 1.0 HA C:PRO36 3.5 17.7 1.0 HB C:THR31 3.6 11.7 1.0 HA C:PRO33 3.6 16.5 1.0 CZ B:ARG133 3.8 34.6 1.0 C C:SER35 3.8 11.8 1.0 NH2 B:ARG133 3.9 34.2 1.0 C C:ILE32 3.9 10.1 1.0 H C:SER35 3.9 13.3 1.0 O C:PRO33 4.2 14.3 1.0 H C:ILE32 4.2 13.4 1.0 CA C:PRO33 4.3 13.7 1.0 CA C:PRO36 4.3 14.8 1.0 N C:PRO36 4.4 13.4 1.0 C C:PRO33 4.4 13.2 1.0 HG1 C:THR31 4.4 12.5 1.0 O C:HOH639 4.5 31.2 1.0 CB C:THR31 4.5 9.7 1.0 N C:SER35 4.5 11.1 1.0 N C:PRO33 4.5 13.8 1.0 O C:HOH655 4.6 11.1 1.0 N C:ILE32 4.7 11.1 1.0 HH21 B:ARG133 4.7 41.1 1.0 OG1 C:THR31 4.7 10.4 1.0 CA C:SER35 4.8 11.9 1.0 HG21 C:THR31 4.8 11.6 1.0 NE B:ARG133 4.9 31.0 1.0 CA C:ILE32 5.0 10.0 1.0 HD3 B:ARG133 5.0 34.0 1.0

T.Hibi, T.Itoh, Y.Nishiya. Flexibility of A Distal Interface Loop Modulates Water Network in the Active Site of Bacillus Sp. Tb-90 Urate Oxidase To Be Published.