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Potassium in PDB 5xrz: Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52

Protein crystallography data

The structure of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52, PDB code: 5xrz was solved by M.Saotome, K.Saito, T.Yasuda, S.Sugiyama, H.Kurumizaka, W.Kagawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 45.70 / 3.60
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 67.621, 101.420, 101.710, 91.89, 108.68, 108.17
R / Rfree (%) 21.7 / 25.7

Potassium Binding Sites:

The binding sites of Potassium atom in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 (pdb code 5xrz). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 10 binding sites of Potassium where determined in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52, PDB code: 5xrz:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Potassium binding site 1 out of 10 in 5xrz

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Potassium binding site 1 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K301

b:0.6
occ:1.00
OE2 A:GLU140 2.9 0.3 1.0
OP1 L:DT5 3.7 0.3 1.0
CD A:GLU140 3.8 0.2 1.0
OE2 B:GLU145 3.8 0.6 1.0
OE1 A:GLU140 3.8 0.6 1.0
NH1 B:ARG55 4.1 0.9 1.0
O3' L:DT4 4.2 0.9 1.0
OD1 B:ASP149 4.5 0.2 1.0
P L:DT5 4.6 1.0 1.0
NZ A:LYS144 4.7 1.0 1.0
O B:TYR126 4.7 96.0 1.0
C4' L:DT4 4.8 0.1 1.0
O4' L:DT5 5.0 0.3 1.0
CD B:GLU145 5.0 0.3 1.0

Potassium binding site 2 out of 10 in 5xrz

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Potassium binding site 2 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K301

b:0.1
occ:1.00
OE2 B:GLU140 3.0 0.7 1.0
OE2 C:GLU145 3.7 0.6 1.0
CD B:GLU140 3.8 0.3 1.0
OE1 B:GLU140 3.8 0.6 1.0
O3' L:DT8 4.0 0.4 1.0
C4' L:DT8 4.0 0.2 1.0
C2' L:DT8 4.3 0.2 1.0
NH1 C:ARG55 4.3 0.4 1.0
C3' L:DT8 4.4 0.9 1.0
O4' L:DT8 4.5 0.7 1.0
OD1 C:ASP149 4.6 0.7 1.0
OP1 L:DT9 4.6 0.2 1.0
O4' L:DT9 4.7 0.4 1.0
O C:TYR126 4.8 90.6 1.0
C1' L:DT8 4.8 0.7 1.0
NZ B:LYS144 4.8 81.1 1.0
CD C:GLU145 4.9 0.5 1.0

Potassium binding site 3 out of 10 in 5xrz

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Potassium binding site 3 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K301

b:0.7
occ:1.00
OE2 C:GLU140 2.8 0.1 1.0
O3' L:DT12 3.3 0.2 1.0
C2' L:DT12 3.6 0.8 1.0
NH1 D:ARG55 3.8 0.5 1.0
CD C:GLU140 3.9 0.6 1.0
C3' L:DT12 4.0 0.7 1.0
OE1 C:GLU140 4.2 0.5 1.0
OE2 D:GLU145 4.2 0.2 1.0
O5' L:DT13 4.3 0.5 1.0
P L:DT13 4.4 0.7 1.0
OD1 D:ASP149 4.5 0.6 1.0
C4' L:DT12 4.5 0.9 1.0
OP1 L:DT13 4.7 0.6 1.0
C1' L:DT12 4.7 0.0 1.0
NZ C:LYS144 4.8 0.3 1.0
O4' L:DT12 5.0 0.6 1.0

Potassium binding site 4 out of 10 in 5xrz

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Potassium binding site 4 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
D:K301

b:0.7
occ:1.00
OE2 D:GLU140 2.8 0.2 1.0
OP1 L:DT17 3.5 0.5 1.0
C4' L:DT16 3.5 1.0 1.0
O4' L:DT16 3.5 0.6 1.0
OE2 E:GLU145 3.6 0.7 1.0
C5' L:DT16 3.8 0.1 1.0
CD D:GLU140 3.9 0.8 1.0
OE1 D:GLU140 4.2 0.2 1.0
OD1 E:ASP149 4.3 0.8 1.0
NH1 E:ARG55 4.5 0.4 1.0
C1' L:DT16 4.5 0.1 1.0
O E:TYR126 4.6 0.5 1.0
O5' L:DT16 4.7 0.5 1.0
CD E:GLU145 4.7 0.1 1.0
NZ D:LYS144 4.7 0.5 1.0
P L:DT17 4.8 0.5 1.0
C3' L:DT16 4.8 0.8 1.0
C4' L:DT17 4.9 0.4 1.0
CG E:ASP149 4.9 1.0 1.0

Potassium binding site 5 out of 10 in 5xrz

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Potassium binding site 5 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
E:K301

b:0.1
occ:1.00
OE2 E:GLU140 3.1 0.9 1.0
OE2 F:GLU145 3.5 0.8 1.0
OD1 F:ASP149 3.9 0.1 1.0
O3' L:DC20 4.0 0.1 1.0
CD E:GLU140 4.0 0.2 1.0
OP1 L:DC21 4.1 0.8 1.0
O F:TYR126 4.2 0.9 1.0
C4' L:DC20 4.2 0.5 1.0
OE1 E:GLU140 4.3 0.8 1.0
CG F:ASP149 4.5 0.4 1.0
NZ E:LYS144 4.5 0.5 1.0
C3' L:DC20 4.6 0.8 1.0
CD F:GLU145 4.6 0.8 1.0
C2' L:DC20 4.7 0.0 1.0
OP1 L:DC20 4.8 0.0 1.0
P L:DC21 4.8 0.7 1.0
O4' L:DC20 4.8 0.7 1.0
NH1 F:ARG55 4.9 0.9 1.0
CG F:GLU145 4.9 0.1 1.0
OD2 F:ASP149 5.0 0.4 1.0

Potassium binding site 6 out of 10 in 5xrz

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Potassium binding site 6 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
F:K301

b:0.0
occ:1.00
OE2 F:GLU140 2.8 0.2 1.0
OP1 L:DT25 3.3 0.1 1.0
OE2 G:GLU145 3.7 0.0 1.0
OP2 L:DT25 3.9 0.9 1.0
CD F:GLU140 3.9 0.6 1.0
P L:DT25 3.9 0.9 1.0
O5' L:DT25 4.2 0.6 1.0
OD1 G:ASP149 4.2 0.2 1.0
OE1 F:GLU140 4.3 0.0 1.0
NH1 G:ARG55 4.4 0.7 1.0
O G:TYR126 4.5 88.4 1.0
O4' L:DT25 4.7 0.4 1.0
NZ F:LYS144 4.7 0.8 1.0
C4' L:DT25 4.8 0.5 1.0
CG G:ASP149 4.8 0.8 1.0
CD G:GLU145 4.9 1.0 1.0

Potassium binding site 7 out of 10 in 5xrz

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Potassium binding site 7 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 7 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
G:K301

b:0.9
occ:1.00
OE2 G:GLU140 2.8 0.7 1.0
O3' L:DT28 3.7 0.1 1.0
OE2 H:GLU145 3.8 0.5 1.0
CD G:GLU140 3.8 0.3 1.0
C4' L:DT28 4.0 0.5 1.0
OE1 G:GLU140 4.1 0.8 1.0
C2' L:DT28 4.2 0.6 1.0
C3' L:DT28 4.2 0.7 1.0
NH1 H:ARG55 4.3 0.9 1.0
OD1 H:ASP149 4.3 0.1 1.0
OP1 L:DT29 4.3 0.8 1.0
O4' L:DT28 4.5 0.3 1.0
P L:DT29 4.7 0.6 1.0
O H:TYR126 4.8 81.1 1.0
C1' L:DT28 4.8 0.8 1.0
NZ G:LYS144 4.8 0.3 1.0
C4' L:DT29 4.9 0.4 1.0
CD H:GLU145 4.9 0.3 1.0
CG H:ASP149 4.9 97.5 1.0
O4' L:DT29 5.0 0.1 1.0

Potassium binding site 8 out of 10 in 5xrz

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Potassium binding site 8 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 8 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
H:K301

b:0.7
occ:1.00
OE2 H:GLU140 2.9 96.8 1.0
OE2 I:GLU145 3.3 0.1 1.0
OP1 L:DT33 3.7 0.8 1.0
CD H:GLU140 3.9 0.3 1.0
OE1 H:GLU140 4.0 92.3 1.0
NH1 I:ARG55 4.4 0.3 1.0
CD I:GLU145 4.5 0.9 1.0
OD1 I:ASP149 4.6 0.1 1.0
O I:TYR126 4.7 88.5 1.0
C4' L:DT33 4.8 1.0 1.0
C5' L:DT33 5.0 0.3 1.0

Potassium binding site 9 out of 10 in 5xrz

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Potassium binding site 9 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 9 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
I:K301

b:0.7
occ:1.00
OP1 L:DT37 2.9 0.9 1.0
OE2 I:GLU140 2.9 0.2 1.0
OE2 J:GLU145 3.6 0.5 1.0
CD I:GLU140 3.9 0.9 1.0
OE1 I:GLU140 4.1 97.9 1.0
P L:DT37 4.1 0.6 1.0
O3' L:DT36 4.2 0.2 1.0
OD1 J:ASP149 4.3 0.6 1.0
O J:TYR126 4.5 80.3 1.0
NH1 J:ARG55 4.7 0.5 1.0
NZ I:LYS144 4.7 0.3 1.0
CD J:GLU145 4.7 0.1 1.0
O5' L:DT37 4.8 0.3 1.0
CG J:ASP149 4.8 0.8 1.0

Potassium binding site 10 out of 10 in 5xrz

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Potassium binding site 10 out of 10 in the Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 10 of Structure of A Ssdna Bound to the Inner Dna Binding Site of RAD52 within 5.0Å range:
probe atom residue distance (Å) B Occ
K:K301

b:0.9
occ:1.00
OE2 K:GLU140 2.8 0.6 1.0
O5' L:DT1 3.3 0.9 1.0
NH1 A:ARG55 3.9 0.5 1.0
CD K:GLU140 4.0 0.7 1.0
OD1 A:ASP149 4.0 0.3 1.0
NZ K:LYS144 4.2 0.1 1.0
C5' L:DT1 4.3 0.5 1.0
OE1 K:GLU140 4.4 0.9 1.0
C4' L:DT1 4.4 0.1 1.0
O4' L:DT1 4.6 0.2 1.0
CG A:ASP149 4.7 0.2 1.0
OE2 A:GLU145 4.8 1.0 1.0

Reference:

M.Saotome, K.Saito, T.Yasuda, H.Ohtomo, S.Sugiyama, Y.Nishimura, H.Kurumizaka, W.Kagawa. Structural Basis of Homology-Directed Dna Repair Mediated By RAD52 Iscience V. 3 50 2018.
ISSN: ESSN 2589-0042
PubMed: 30428330
DOI: 10.1016/J.ISCI.2018.04.005
Page generated: Mon Aug 12 15:09:48 2024

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