Potassium in PDB 5vkh: Closed Conformation of Kcsa Y82A-F103A Mutant

The structure of Closed Conformation of Kcsa Y82A-F103A Mutant, PDB code: 5vkh was solved by L.G.Cuello, E.Perozo, D.M.Cortes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.91 / 2.25
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	155.737, 155.737, 76.225, 90.00, 90.00, 90.00
R / Rfree (%)	17.4 / 20.3

The binding sites of Potassium atom in the Closed Conformation of Kcsa Y82A-F103A Mutant (pdb code 5vkh). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 6 binding sites of Potassium where determined in the Closed Conformation of Kcsa Y82A-F103A Mutant, PDB code: 5vkh:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6;

Potassium binding site 1 out of 6 in the Closed Conformation of Kcsa Y82A-F103A Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Closed Conformation of Kcsa Y82A-F103A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:K1002 b:16.6 occ:0.25 O C:TYR78 2.8 15.3 1.0 O C:GLY77 3.2 17.7 1.0 K C:K1003 3.3 15.6 0.2 C C:TYR78 3.4 14.2 1.0 CA C:TYR78 4.1 13.9 1.0 N C:GLY79 4.2 18.3 1.0 C C:GLY77 4.3 16.9 1.0 CA C:GLY79 4.5 16.1 1.0 N C:TYR78 4.7 13.5 1.0

Potassium binding site 2 out of 6 in the Closed Conformation of Kcsa Y82A-F103A Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Closed Conformation of Kcsa Y82A-F103A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:K1003 b:15.6 occ:0.25 O C:GLY77 2.8 17.7 1.0 O C:VAL76 2.9 14.6 1.0 K C:K1004 3.3 17.4 0.2 K C:K1002 3.3 16.6 0.2 C C:GLY77 3.6 16.9 1.0 C C:VAL76 4.0 17.6 1.0 CA C:GLY77 4.2 19.0 1.0 N C:TYR78 4.5 13.5 1.0 N C:GLY77 4.5 18.8 1.0 CA C:TYR78 4.8 13.9 1.0

Potassium binding site 3 out of 6 in the Closed Conformation of Kcsa Y82A-F103A Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Closed Conformation of Kcsa Y82A-F103A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:K1004 b:17.4 occ:0.25 O C:THR75 2.7 18.2 1.0 O C:VAL76 3.0 14.6 1.0 K C:K1003 3.3 15.6 0.2 K C:K1005 3.3 20.4 0.2 C C:VAL76 3.6 17.6 1.0 C C:THR75 3.8 14.3 1.0 CA C:VAL76 4.0 15.3 1.0 N C:VAL76 4.4 15.7 1.0 N C:GLY77 4.6 18.8 1.0

Potassium binding site 4 out of 6 in the Closed Conformation of Kcsa Y82A-F103A Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Closed Conformation of Kcsa Y82A-F103A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:K1005 b:20.4 occ:0.25 OG1 C:THR75 2.8 17.5 1.0 O C:THR75 3.0 18.2 1.0 K C:K1004 3.3 17.4 0.2 CB C:THR75 3.6 16.2 1.0 C C:THR75 3.8 14.3 1.0 CA C:THR75 4.4 17.1 1.0 N C:VAL76 4.8 15.7 1.0 O C:THR74 4.8 13.8 1.0 CG2 C:THR75 4.8 15.5 1.0

Potassium binding site 5 out of 6 in the Closed Conformation of Kcsa Y82A-F103A Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Closed Conformation of Kcsa Y82A-F103A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:K1006 b:16.8 occ:0.25 O C:HOH1146 3.2 51.1 1.0 O C:HOH1145 3.4 40.4 1.0

Potassium binding site 6 out of 6 in the Closed Conformation of Kcsa Y82A-F103A Mutant


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Closed Conformation of Kcsa Y82A-F103A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ C:K1007 b:42.8 occ:0.25 O C:HOH1148 3.1 62.0 1.0

L.G.Cuello, D.M.Cortes, E.Perozo. The Gating Cycle of A K+ Channel at Atomic Resolution. Elife V. 6 2017.
ISSN: ESSN 2050-084X
PubMed: 29165243
DOI: 10.7554/ELIFE.28032