Potassium in PDB 5uas: Structure of A New Family of Polysaccharide Lyase PL25-Ulvanlyase Bound to -[Glca(1-4)RHA3S]-

The structure of Structure of A New Family of Polysaccharide Lyase PL25-Ulvanlyase Bound to -[Glca(1-4)RHA3S]-, PDB code: 5uas was solved by T.S.Ulaganathan, M.Cygler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.80 / 1.60
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	173.620, 71.810, 109.270, 90.00, 122.44, 90.00
R / Rfree (%)	18.2 / 21.2

The structure of Structure of A New Family of Polysaccharide Lyase PL25-Ulvanlyase Bound to -[Glca(1-4)RHA3S]- also contains other interesting chemical elements:

Zinc	(Zn)	2 atoms
Chlorine	(Cl)	2 atoms

The binding sites of Potassium atom in the Structure of A New Family of Polysaccharide Lyase PL25-Ulvanlyase Bound to -[Glca(1-4)RHA3S]- (pdb code 5uas). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Structure of A New Family of Polysaccharide Lyase PL25-Ulvanlyase Bound to -[Glca(1-4)RHA3S]-, PDB code: 5uas:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Structure of A New Family of Polysaccharide Lyase PL25-Ulvanlyase Bound to -[Glca(1-4)RHA3S]-


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Structure of A New Family of Polysaccharide Lyase PL25-Ulvanlyase Bound to -[Glca(1-4)RHA3S]- within 5.0Å range: probe atom residue distance (Å) B Occ A:K602 b:16.4 occ:1.00 O A:HOH1128 3.1 12.0 1.0 O A:HOH964 3.2 17.1 1.0 O A:HOH1058 3.2 13.6 1.0 O A:HOH1061 3.3 14.3 1.0 N A:ILE64 3.3 13.6 1.0 CB A:ILE64 3.8 10.5 1.0 CE2 A:PHE329 4.0 13.1 1.0 CD1 A:ILE64 4.0 12.0 1.0 CG1 A:ILE64 4.0 12.5 1.0 CA A:ALA63 4.1 11.9 1.0 CB A:ALA63 4.2 12.6 1.0 O A:HOH817 4.2 13.8 1.0 CA A:ILE64 4.2 10.2 1.0 C A:ALA63 4.2 13.0 1.0 CD A:ARG386 4.3 12.8 1.0 CG A:ARG386 4.4 14.4 1.0 O A:HOH927 4.4 16.7 1.0 NE A:ARG386 4.6 13.1 1.0 O A:ILE439 4.6 14.1 1.0 CB A:ARG386 4.7 13.5 1.0 CZ A:PHE329 4.7 13.8 1.0 O A:HOH853 4.9 13.4 1.0 O A:HOH942 4.9 13.1 1.0 N A:VAL65 5.0 10.3 1.0 CD2 A:PHE329 5.0 13.9 1.0 O A:HOH1130 5.0 23.8 1.0

Potassium binding site 2 out of 2 in the Structure of A New Family of Polysaccharide Lyase PL25-Ulvanlyase Bound to -[Glca(1-4)RHA3S]-


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Structure of A New Family of Polysaccharide Lyase PL25-Ulvanlyase Bound to -[Glca(1-4)RHA3S]- within 5.0Å range: probe atom residue distance (Å) B Occ B:K602 b:17.0 occ:1.00 O B:HOH922 3.0 16.6 1.0 O B:HOH1065 3.2 15.4 1.0 O B:HOH1021 3.2 12.9 1.0 O B:HOH1009 3.3 14.7 1.0 N B:ILE64 3.3 12.4 1.0 CB B:ILE64 3.8 11.1 1.0 CD1 B:ILE64 3.9 13.9 1.0 CG1 B:ILE64 3.9 11.2 1.0 CE2 B:PHE329 4.0 14.9 1.0 CB B:ALA63 4.1 12.9 1.0 CA B:ALA63 4.1 11.6 1.0 CA B:ILE64 4.2 12.7 1.0 O B:HOH818 4.2 14.4 1.0 CD B:ARG386 4.2 12.6 1.0 C B:ALA63 4.2 11.7 1.0 O B:HOH956 4.4 18.4 1.0 CG B:ARG386 4.4 13.1 1.0 NE B:ARG386 4.6 14.1 1.0 O B:ILE439 4.6 13.0 1.0 CB B:ARG386 4.6 14.8 1.0 CZ B:PHE329 4.8 13.8 1.0 O B:HOH891 4.9 14.2 1.0 O B:HOH925 5.0 16.1 1.0 N B:VAL65 5.0 10.7 1.0 CD2 B:PHE329 5.0 13.8 1.0

T.Ulaganathan, M.T.Boniecki, E.Foran, V.Buravenkov, N.Mizrachi, E.Banin, W.Helbert, M.Cygler. New Ulvan-Degrading Polysaccharide Lyase Family: Structure and Catalytic Mechanism Suggests Convergent Evolution of Active Site Architecture. Acs Chem. Biol. V. 12 1269 2017.
ISSN: ESSN 1554-8937
PubMed: 28290654
DOI: 10.1021/ACSCHEMBIO.7B00126